Immunocytochemical demonstration of amelogenins and enamelins secreted by ameloblasts during the secretory and maturation stages.

Inage, Toshihiko; Shimokawa, Hitoyata; Teranishi, Yasuhiro; Igarashi, Takashi; Toda, Yoshihisa; Moro, Itaru

doi:10.1679/aohc.52.213

Cited by 54 publications

(47 citation statements)

References 33 publications

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“…Finally, most of the matrix (specifically the amelogenins) and the fluid have been replaced gradually and the mineral volume fraction component increases steeply from 10% -20% to 80% -90%. It should be noted that ameloblasts continue to secrete some proteins well into the maturation period (Inage et al, 1989).…”

Section: Formation and Characteristics Of Enamelmentioning

confidence: 99%

Understanding the mechanical behaviour of human enamel from its structural and compositional characteristics

Swain

2008

Journal of the Mechanical Behavior of Biomedical Materials

321

157

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Section: Formation and Characteristics Of Enamelmentioning

confidence: 99%

Understanding the mechanical behaviour of human enamel from its structural and compositional characteristics

Swain

2008

Journal of the Mechanical Behavior of Biomedical Materials

321

157

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“…Bars equal 0.5 pm. creted enamel protein, since in rodents it is specifically immunoreactive to an anti-amelogenin antibody (Slavkin et al, 1988;Inage et al, 1989;Nanci et al, 1989). Occasionally associated with, or near, these globules and mineralized collagen fibrils were thin ribbons of organic material that outlined the characteristic profile of enamel crystallites as seen in mineralized tissue sections.…”

Section: Electron Microscopy Of the Enamel Organmentioning

confidence: 99%

Morphology of the enamel organ in the miniature swine

McKee¹,

Aoba²,

Moreno³

1991

Anat. Rec.

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In recent years, the dentition of the pig has been increasingly used as a model for the study of amelogenesis. Indeed, much of our current knowledge on enamel formation derives from biochemical and physicochemical analyses of the organic and inorganic components, respectively, of porcine enamel. As an extension of this previous work, and as the first step in our attempt to correlate known enamel matrix and mineral changes with adjacent enamel organ morphology, the present study was undertaken to provide a description of the morphological events occurring in the enamel organ during porcine amelogenesis. Two-week-old miniature swine (minipigs) were fixed by vascular perfusion with glutaraldehyde, the deciduous teeth present at this age were embedded in Epon resin and sectioned, and the cells of the enamel organ at each of the various developmental stages of amelogenesis were examined by light and transmission electron microscopy. In many respects, the morphology of the porcine enamel organ was similar to that previously described in other mammalian species. On the other hand, several particularities were noted and these are discussed in the context of available data correlating cell ultrastructure with putative function during enamel formation.

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“…It may be assumed that the degraded enamel matrix proteins are absorbed by ameloblasts at these stages, although they may still continue to synthesize some lower level proteins (Inage et al 1989(Inage et al , 1996Smith et al 1992;Smith andNanci 1995, 1996;Nanci et al 1998;Smith 1998). Enamel matrix-like materials or materials that are immunoreactive to enamel matrix proteins have been observed in the intercellular spaces of ameloblasts and papillary layers (Yamamoto et al 1984;Nanci et al 1987Nanci et al , 1992.…”

Section: Introductionmentioning

confidence: 99%

Internalization of amelogenin by dendritic cells of the papillary layer during transition and early maturation stages

Nishikawa

Sasaki

1999

Histochemistry and Cell Biology

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The existence of amelogenin in the papillary layer facing transition and early maturation zones of ameloblasts of rat incisors and the role of dendritic cells were examined by light microscopic immunocytochemistry and immunoelectron microscopy using anti-porcine 25 kDa amelogenin antibodies and anti-MHC class II antibodies. At the light microscopic level, no overall relationship was observed between anti-class II-positive dendritic cells and anti-amelogenin-positive materials located intercellularly: anti-amelogenin-positive dots were scattered in papillary layers and ameloblasts. Anti-MHC class II-positive cells were present in the papillary layer at the transition and maturation stages. Under electron microscopy, however, the dendritic cells occasionally endocytosed anti-amelogenin-positive materials and formed small vesicles. The results suggest that the dendritic cells play a role in eliminating amelogenin from the enamel organ.

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Immunocytochemical demonstration of amelogenins and enamelins secreted by ameloblasts during the secretory and maturation stages.

Abstract: Summary.Rabbit polyclonal antibodies against bovine amelogenins and enamelins which did not show any cross-reaction were raised, and ultrathin sections of rat incisors were examined by the protein A-gold and ABC methods.

Cited by 54 publications

References 33 publications

Understanding the mechanical behaviour of human enamel from its structural and compositional characteristics

Understanding the mechanical behaviour of human enamel from its structural and compositional characteristics

Morphology of the enamel organ in the miniature swine

Internalization of amelogenin by dendritic cells of the papillary layer during transition and early maturation stages

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