Immunocytochemical demonstration of neuropeptides in the nervous system of the liver fluke, <i>Fasciola hepatica</i> (Trematoda, Digenea)

Magee, R.J.; Fairweather, Ian; Johnston, C.F.; Halton, D W; Shaw, Christopher

doi:10.1017/s0031182000062132

Cited by 84 publications

(61 citation statements)

References 52 publications

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“…6E). Neuronal cell bodies, similar both in number and immunoreactivity for FaRP, surround the ootypes of Fasciola hepatica and Schistosoma mansoni and provide synaptic contacts with the muscle layers and sphincters therein and of related ducts (Magee et al 1989;Marks et al 1995). By 10-12 days p.i., the uteruses of all E. caproni examined (n=10) were invariably filled with eggs, and there was strong immunoreactivity for FaRP peptide and 5-HT in the innervation of the ootype.…”

Section: Post-ovigerous Adultsmentioning

confidence: 99%

The musculature and associated innervation of adult and intramolluscan stages of Echinostoma caproni (Trematoda) visualised by confocal microscopy

et al. 2004

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Gross anatomy of muscle and sensory/motor innervation of adult and intramolluscan developmental stages of Echinostoma caproni have been investigated to ascertain the organisation and the functional correlates of any stage-specific patterns of staining. Using indirect immunocytochemistry to demonstrate neuroactive substances and the phalloidin-fluorescence technique for staining myofibril F-actin, the muscle systems and aminergic and peptidergic innervation of daughter rediae, cercariae, metacercariae, and pre- and post-ovigerous adults were examined and compared using confocal scanning laser microscopy. A complex arrangement of specific muscle fibre systems occurs within the body wall (composed of circular, longitudinal and diagonal fibres), suckers (radial, equatorial, meridional), pharynx (radial, circular), gut caeca (mainly circular), cercarial tail (circular, pseudo-striated longitudinal), and ducts of the reproductive system (circular, longitudinal), presumed to serve locomotor, adhesive, alimentary and reproductive functions. Immunostaining for serotonin (5-HT) and FMRFamide-related peptides (FaRPs) was evident throughout the central (CNS) and peripheral (PNS) nervous systems of all stages, and use of dual-labelling techniques demonstrated separate neuronal pathways for 5-HT and FaRP in both CNS and PNS. FaRP expression in the innervation of the ootype wall was demonstrated only in post-ovigerous worms and not in pre-ovigerous worms, suggesting an involvement of FaRP neuropeptides in the process of egg assembly. Comparison of the present findings with those recorded for other digeneans suggests that muscle organisation and innervation patterns in trematodes are highly conserved.

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Section: Post-ovigerous Adultsmentioning

confidence: 99%

The musculature and associated innervation of adult and intramolluscan stages of Echinostoma caproni (Trematoda) visualised by confocal microscopy

et al. 2004

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“…All of these adhesive organs are richly innervated with fine nerve plexuses. Similarly, the oral and ventral suckers of the trematodes, Fasciola hepatica, Haplometra cylindracea, Schistosoma mansoni, Corriga vitta and Gorgoderina vitelliloba, are provided with a multiplicity of serotoninergic and peptidergic nerves that emanate from the MCs (ventral nerve cords) and anastomose as plexuses of fine fibres among the muscle bands (Magee et al 1989;McKay et al 1990, 19916;Skuce et al 1990;Magee et al 1993;Marks et al 1995).…”

Section: Attachment Organsmentioning

confidence: 99%

Functional morphology of the platyhelminth nervous system

1996

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As the most primitive metazoan phylum, the Platyhelminthes occupies a unique position in nervous system evolution. Centrally, their nervous system consists of an archaic brain from which emanate one or more pairs of longitudinal nerve cords connected by commissures; peripherally, a diverse arrangement of nerve plexuses of varying complexity innervate the subsurface epithelial and muscle layers, and in the parasitic taxa they are most prominent in the musculature of the attachment organs and egg-forming apparatus. There is a range of neuronal-cell types, the majority being multi-and bipolar. The fiatworm neuron is highly secretory and contains a heterogeneity of vesicular inclusions, dominated by densecored vesicles, whose contents may be released synaptically or by paracrine secretion for presumed delivery to target cells via the extracellular matrix. A wide range of sense organ types is present in flatworms, irrespective of life-styles. The repertoire of neuronal substances identified cytochemically includes all of the major candidate transmitters known in vertebrates. Two groups of native fiatworm neuropeptides have been sequenced, neuropeptide F and FMRFamide-related peptides (FaRPs), and immunoreactivities for these have been localised in dense-cored neuronal vesicles in representatives of all major fiatworm groups. There is evidence of co-localisation of peptidergic and cholinergic elements; serotoninergic components generally occupy a separate set of neurons. The actions of neuronal substances in flatworms are largely undetermined, but FaRPs and S-HT are known to be myoactive in all of the major groups, and there is immunocytochemical evidence that they have a role in the mechanism of egg assembly.

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“…Regulatory peptides were first discovered in representatives of the major flatworm groups in the 1980s, with findings recorded for the turbellarian, Microstomum lineare by Reuter et al (1980); the cestode, Diphyllobothrium dendriticum by Gustafsson et al (1985); the trematode, Fasciola hepatica by Magee et al (1989); and the monogenean, Diclidophora merlangi by Maule et al (1989). To date, the most extensive and intense peptide immunostaining demonstrated in all classes of the platyhelminths has been obtained with antisera raised to the native invertebrate neuropeptide, FMRFamide, and to invertebrate members of the neuropeptide Y (NPY) superfamily, notably neuropeptide F (NPF; Halton et al, 1994).…”

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confidence: 99%

The peptidergic nervous system of the triclad turbellarian,Bdelloura candida (maricola, bdellouridae): An immunocytochemical study using an antiserum raised to an endogenous neuropeptide, GYIRFamide

Johnston¹,

Halton²,

Anderson

et al. 1996

J. Comp. Neurol.

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The organisation of the nervous system of Bdelloura candida (Tricladida, Maricola, and Bdellouridae) was studied by immunocytochemistry, by using an antiserum raised to the authentic B. candida FMRFamide-related peptide (FaRP), GYIRFamide. Immunostaining was intense and abundant throughout both the central and peripheral nervous systems, being localised to the brain, the longitudinal nerve cords and their transverse and lateral connections, the pharyngeal plexus, the extensive sub-epidermal and sub-muscular plexuses, and elements of the reproductive apparatus. Compared to an earlier anatomical investigation of this species, and also to the neuroanatomy of other triclad turbellarians, the pattern of GYIRFamide-immunoreactivity reveals differences in the following aspects: the shape and structure of the brain, the distribution of longitudinal nerve cords and their relationships with the peripheral nervous system, the structure and distribution of the lateral nerves and the transverse connectives between the longitudinal nerve cords, organisation of the pharyngeal nervous system, and innervation of the eyespots and epidermal sensory structures. Although this study focuses on a descriptive account of the neuroanatomy of Bdelloura candida, by using anti-GYIRFamide as a neuronal marker, the possible functions of the native peptide are also discussed. The quality and reproducibility of the immunostaining obtained during this work highlights the effectiveness of the GYIRFamide antiserum in the neuroanatomical study of flatworms, and also the suitability of B. candida as a model species in studies of the turbellarian nervous system.

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Immunocytochemical demonstration of neuropeptides in the nervous system of the liver fluke, Fasciola hepatica (Trematoda, Digenea)

Cited by 84 publications

References 52 publications

The musculature and associated innervation of adult and intramolluscan stages of Echinostoma caproni (Trematoda) visualised by confocal microscopy

The musculature and associated innervation of adult and intramolluscan stages of Echinostoma caproni (Trematoda) visualised by confocal microscopy

Functional morphology of the platyhelminth nervous system

The peptidergic nervous system of the triclad turbellarian,Bdelloura candida (maricola, bdellouridae): An immunocytochemical study using an antiserum raised to an endogenous neuropeptide, GYIRFamide

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