Immunocytochemical localization of epidermal growth factor in mouse submandibular gland.

Gresik, Edward W.; Barka, Tibor

doi:10.1177/25.9.333017

Cited by 131 publications

(71 citation statements)

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“…In these hypertrophic GCT cells the amount of mK1 was decreased by the action of androgen. Slight and infrequent intercellular and intergranular variations in staining intensity have been reported for EGF, NGF, and renin in GCT cells (Gresik and Barka 1977;Gresik et al 1978a;Tanaka et al 1981;Kusakabe et al 1986), but not to the degree seen here for mK1. Moreover, these variations in immunostaining are not sexually dimorphic and are clearly different from the sexual variation in mK1 distribution.…”

Section: Discussionmentioning

confidence: 65%

Developmental and Androgenic Regulation of the Immunocytochemical Distribution of mK1, a True Tissue Kallikrein, in the Granular Convoluted Tubule of the Mouse Submandibular Gland

Kurabuchi

Hosoi

Gresik

2002

J Histochem Cytochem.

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S U M M A R YThe action of androgens on the immunocytochemical distribution of mK1, a true tissue kallikrein, was examined in the submandibular gland (SMG) of developing and adult mice by indirect enzyme-labeled and immunogold-labeled antibody methods for light and electron microscopy, respectively. In both sexes at 3 weeks of age, essentially all of the immature granular convoluted tubule (GCT) cells were uniformly immunostained. At 4 weeks of age (the onset of puberty), morphological differences between the two sexes appeared in the GCTs, in which some cells became immunonegative. Thereafter, the immunonegative GCT cells became more abundant in the SMG of males than of females and considerable intercellular variation in staining intensity for mK1 was seen, especially in males. A few slender GCT cells with strong immunoreactivity appeared in GCT segments only in males. Castration of males resulted in an increase in the number of immunopositive GCT cells, whereas administration of dihydrotestosterone (DHT) decreased the number of immunopositive GCT cells in the SMGs of both sexes. Slender GCT cells immunoreactive for mK1 were seen in females treated with DHT for 6 days. However, there were no immunostained slender GCT cells in female SMGs after injection of DHT for 2 weeks. Immunoelectron microscopy disclosed this type of cell in male SMGs, which closely resembles immature GCT cells of prepubertal mice, with a few small secretory granules uniformly labeled with gold particles, a sparse Golgi apparatus and RER, and basal infoldings. In mature male SMGs and in SMGs of DHT-treated females and castrated males, typical GCT cells had a well-developed Golgi apparatus and a net-like RER but few to no basal infoldings, whereas in the female gland equivalent cells had moderately developed RER and some basal infoldings. These results suggest that mK1 is one of the enzymes characteristically present in immature GCT cells and that its synthesis is inhibited in part by androgens, resulting in decreased numbers of immunopositive cells.

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Section: Discussionmentioning

confidence: 65%

Developmental and Androgenic Regulation of the Immunocytochemical Distribution of mK1, a True Tissue Kallikrein, in the Granular Convoluted Tubule of the Mouse Submandibular Gland

Kurabuchi

Hosoi

Gresik

2002

J Histochem Cytochem.

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“…The biological significances of rodent submandibular glands have been focused on the biological active polypeptide producing system in GCT cells which showed sex dimorphism (1,6,16,22,23). In postnatal salivary glands, GCT cells were not developed just after birth; GCT cells appeared at 15 to 20 days of age in the mouse and 30 to 40 days of age in the rat.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical expression of chromogranin in postnatal developmental salivary glands of mice and rats.

Kunikata

Yamada

Ogata

et al. 1992

Acta Histochem. Cytochem

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The immunoreactivity of chromogranin was studied in postnatal salivary glands of the mice and rats. Chromogranin was observed in proacinar cells in both sexes during the postnatal developmental stage of the submandibular glands; the strongest reactivity was seen in 4th to 7th day specimens of mouse, and in 7th to 21st day specimens of rat. Acinar cells of mouse parotid glands showed positive staining in 1 to 42 days. No chromogranin staining was seen in granular convoluted tubule (GCT) cells of the submandibular glands in mice and rats. In sublingual glands of rat, reactivity of chromogranin was found in undifferentiated serous acinar cells, whereas in mice, it was absent in these cells. No sex difference of chromogranin staining was observed in submandibular glands in mice and rats. Immunoblotting of chromogranin using polyclonal anti-bovine SP-1/chromogranin antiserum showed in submandibular glands and adrenal glands of the rat consisted of chromogranin A, B and C. The results of this study suggest that the existence of chromogranin in proacinar cells of salivary glands is probably associated with the developmental phenomenon during the postnatal development.

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“…GCT cells in the salivary glands are unique cells which markedly exist in the SMG in mature male mice, and secretory granules are the main source of salivary gland growth factors such as EGF and NGF (1,8,12). GCT cells in rodent SMGs were found in mice, rats and hamsters, and EGF also exists in the rat GCT cells, however, the biological roles of hamster GCT cells are not yet understood.…”

Section: Discussionmentioning

confidence: 99%

“…In GCT cells in murine SMGs (mouse, rat, and hamster) particular structures exist for biosynthesis and storage of salivary gland growth factors (EGF and NGF), renin, kallikrein and esteroprotease (1,7,8,12,18). The preceding papers have reported that ductal segments of hamster SMGs were crossreacted with mouse EGF (11) and slightly positive to keratin proteins from immunohistochemical method (20).…”

Section: Immunocytochemicalmentioning

confidence: 99%

Vimentin characteristically exists in granular convoluted tubules of hamster submandibular glands. Immunohistochemical studies on comparative localization of filament proteins in salivary gland ducts.

Mori

Takai

Hosaka

et al. 1985

Acta Histochem. Cytochem

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Immunocytochemicaldetection of vimentin class of filament proteins except for keratin is reported in ductal segments of rodent submandibular glands (SMGs) and compared to stainabilities of different ductal systems; intercalated duct (ICD), granular convoluted tubule (GCT), striated duct (SD) and excretory duct (ED), and in the major salivary glands; parotid (PG), submandibular (SMG) and sublingual glands (SLG). Filament proteins; vimentin, desmin, filamin, actin and myosin were examined by immunocytochemical technique. Vimentin characteristically was confined to the GCT cells of hamster SMGs, and was distributed in luminal surface of the SD cells of guinea pigs, where it was negative in the SMGs of rats and mice, but positive in SLG duct cells. Vimentin was also positive in the ICD cells of hamster SLG.Desmin was slightly positive in the duct cells of hamsters, and intensely positive in luminal side of the SD cells of SMG and PG in guinea pigs, and of SLG in rats. Trace amounts of desmin were also noted in the GCT cells of mice and rats. Filamin was strongly positive in smooth muscles of blood vessels and trace or slightly positive in ductal cells from all the specimens.Actin was also strongly evident in smooth muscles, and slightly reactive to hamsters GCT cells, guinea pigs, and rats ductal cells and was negative in mice. Myosin was slightly positive in the GCT cells of hamsters and in the SD cells of guinea pig.

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Immunocytochemical localization of epidermal growth factor in mouse submandibular gland.

Cited by 131 publications

References 0 publications

Developmental and Androgenic Regulation of the Immunocytochemical Distribution of mK1, a True Tissue Kallikrein, in the Granular Convoluted Tubule of the Mouse Submandibular Gland

Developmental and Androgenic Regulation of the Immunocytochemical Distribution of mK1, a True Tissue Kallikrein, in the Granular Convoluted Tubule of the Mouse Submandibular Gland

Immunohistochemical expression of chromogranin in postnatal developmental salivary glands of mice and rats.

Vimentin characteristically exists in granular convoluted tubules of hamster submandibular glands. Immunohistochemical studies on comparative localization of filament proteins in salivary gland ducts.

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