Immunocytochemical localization of histamine in flatworms

Wikgren, Marianne; Reuter, Maria; Gustafsson, Margaretha K. S.; Lindroos, Paula

doi:10.1007/bf00297227

Cited by 39 publications

(19 citation statements)

References 18 publications

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“…The existence of prolactin immunoreactive nerve cell bodies and fibres on the surface of the main excretory duct was observed in our previous study (Liu et al, 1996). 5-HT, peptide histidine isloeucine and histoaminergic immunoreactive cell bodies and fibres were also demonstrated in the wall of the ducts (Gustafsson et al, 1986and Wikgren et al, 1990). This finding and previous results suggest that neurosecretory cells are involved in the regulation of the excretory activity.…”

Section: Discussionsupporting

confidence: 58%

Ultrastructural Study of Neurosecretory Cells in the Nervous System in the Cestode (Taenia hydatigena)

Liu

Wakuri²,

Mutoh³

et al. 1996

Okajimas Folia Anatomica Japonica

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Section: Discussionsupporting

confidence: 58%

Ultrastructural Study of Neurosecretory Cells in the Nervous System in the Cestode (Taenia hydatigena)

Liu

Wakuri²,

Mutoh³

et al. 1996

Okajimas Folia Anatomica Japonica

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“…The data of histamine in the brain of other invertebrates are very limited. Wikgren et al (1990) demonstrated in different members of the Plathelminthes the occurrence of histamine in the nervous system and the distribution of histamine in the area of the excretory organs. Panula et al (1995) showed immunoreactivity in the region of the cerebral eye of Dugesia tigrina, but observed no histaminergic structures in the nervous system.…”

Section: Histaminementioning

confidence: 98%

Architecture of the nervous system in two Dactylopodola species (Gastrotricha, Macrodasyida)

Rothe

Schmidt‐Rhaesa

2008

Zoomorphology

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Immunohistochemical stainings have become standard tools to describe the nervous system, but usually only singular or few markers are used and consequently show only subsets of neurons within the nervous system. We investigated two species of Dactylopodola (Gastrotricha, Macrodasyida) with a broad set and combination of markers, to represent the nervous system in a more holistic approach. We suggest that markers for both neurotubuli (tubulin) and neurotransmitters (e.g. serotonin, FMRFamides, histamine) should be used. Combinations with markers for the musculature (phalloidin) and nuclei (propidiumiodide or other markers) help to reveal spatial patterns and when used with TEM can provide a more precise picture of the spatial relationships of particular nerves. Species of Dactylopodola have a brain consisting of a solid dorsal commissure and a fine ventral commissure. Cell somata of brain cells are arranged lateral to the dorsal commissure and form a dumbbell-like brain. Additionally, projections into the head region, head sensory organs, one pair of lateroventral nerve cords with three commissures and stomatogastric nerves are described. Obviously, some longitudinal transmitter-specific fibres run in parallel to the main longitudinal nerve and represent additional longitudinal fibres. In comparison with the nervous system architecture of other gastrotrich species and that of different bilaterian animals it is speculated that the gastrotrich nervous system retains several ancestral features, such as being commissural and not a compact brain.

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“…Surprisingly little is known about the biology of HA in flatworms . In a parasitic flatworm Diphyllobothrium dendriticum numerous HA-ir nerve cells and fibres are found laterally to the main nerve cord (Wikgren et al, 1990) . In the microturbellarian Microstomum lineare two symmetrically placed pear-shaped HA-ir neurons are located on each side of the both excretory pores close to the presumptive photoreceptor cells (Palmberg et al, 1980 ;Wikgren et al, 1990) .…”

Section: Discussionmentioning

confidence: 99%

An immunocytochemical method for histamine: application to the planarians

et al. 1995

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Histamine-immunoreactivity was investigated in the planarians Dugesia tigrina and Polycelis nigra . Specific antisera against a histamine-protein conjugate were used, and 1-ethyl-3 (3-dimethyl-aminopropyl) carbodiimide was used both as coupling agent to prepare the antigen and as a tissue fixative . In D. tigrina, histamine-immunoreactivity was restricted to photoreceptor cells in the cerebral eye . In P nigra, nerve fibers were found in the ventral nerve cord and nerves running laterally from these . The epidermal eyes did not display histamine-immunoreactivity . The results suggest that histamine may be a transmitter in some of the most primitive animals. They also suggest that the distribution of histamine may differ in planarians .

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Immunocytochemical localization of histamine in flatworms

Cited by 39 publications

References 18 publications

Ultrastructural Study of Neurosecretory Cells in the Nervous System in the Cestode (Taenia hydatigena)

Ultrastructural Study of Neurosecretory Cells in the Nervous System in the Cestode (Taenia hydatigena)

Architecture of the nervous system in two Dactylopodola species (Gastrotricha, Macrodasyida)

An immunocytochemical method for histamine: application to the planarians

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