Immunocytochemical localization of vasotocin and isotocin in the preopticohypophysial neurosecretory system of teleosts

Goossens, N.; Dierickx, K; Vandesande, Frans

doi:10.1016/0016-6480(77)90216-7

Cited by 105 publications

(24 citation statements)

References 9 publications

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“…Based on the presence of neurohypophysial peptides and axon projection to the neurohypophysis, these are homologous structures (Goossens et al, 1977;Cumming et al, 1982;Swanson and Sawchenko, 1983;Batten et al, 1990;Gainer and Wray, 1994;Holmqvist and Ekstrom, 1995;Saito et al, 2004). However, their differing anatomical locations in the brain raised the question of whether the oxytocin developmental gene regulatory network remained conserved with isotocin in the developing zebrafish brain.…”

Section: Discussionmentioning

confidence: 99%

“…Isotocin, as well as vasotocin, is produced in magnocellular and parvocellular neurons in the preoptic area. These neurons project axons to the neurohypophysis (posterior pituitary) where isotocin (and vasotocin) is released into the systemic circulation (Goossens et al, 1977;Reaves and Hayward, 1980;Cumming et al, 1982;Van den Dungen et al, 1982;Batten et al, 1990;Holmqvist and Ekstrom, 1995;Saito et al, 2004). Thus, the fish preoptico-neurohypophysial system is homologous to the mammalian HNS.…”

Section: Introductionmentioning

confidence: 99%

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The zebrafish bHLH PAS transcriptional regulator, single‐minded 1 (sim1), is required for isotocin cell development

Eaton

Glasgow

2006

Developmental Dynamics

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A wide range of physiological and behavioral processes, such as social, sexual, and maternal behaviors, learning and memory, and osmotic homeostasis are influenced by the neurohypophysial peptides oxytocin and vasopressin. Disruptions of these hormone systems have been linked to several neurobehavioral disorders, including autism, Prader-Willi syndrome, affective disorders, and obsessive-compulsive disorder. Studies in zebrafish promise to reveal the complex network of regulatory genes and signaling pathways that direct the development of oxytocin-and vasopressin-like neurons, and provide insight into factors involved in brain disorders associated with disruption of these systems. Isotocin, which is homologous to oxytocin, is expressed early, in a simple pattern in the developing zebrafish brain. Single-minded 1 (sim1), a member of the bHLH-PAS family of transcriptional regulatory genes, is required for terminal differentiation of mammalian oxytocin cells and is a master regulator of neurogenesis in Drosophila. Here we show that sim1 is expressed in the zebrafish forebrain and is required for isotocin cell development. The expression pattern of sim1 mRNA in the embryonic forebrain is dynamic and complex, and overlaps with isotocin expression in the preoptic area. We provide evidence that the role of sim1 in zebrafish neuroendocrine cell development is evolutionarily conserved with that of mammals.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The zebrafish bHLH PAS transcriptional regulator, single‐minded 1 (sim1), is required for isotocin cell development

Eaton

Glasgow

2006

Developmental Dynamics

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“…preoptic and lateral tuberal nuclei). Plasma AVT and IT levels represent a balance between hormonal secretion from the pituitary and a variety of removal processes, including renal clearance, enzymatic degradation and binding to receptors in various tissues (Goossens et al, 1977;van den Dungen et al, 1982;Acher, 1993;Acher and Chauvet, 1995;Goodson and Bass, 2000).…”

Section: Regulation Of Avt and It Synthesis And Releasementioning

confidence: 99%

“…Furthermore, plasma AVT and IT expression is controlled on multiple levels, including transcription and peptide synthesis, maturation, transport, storage and release. Both hormones initiate their physiological activities by interacting with specific receptors within target tissues (Goossens et al, 1977;van den Dungen et al, 1982;Acher, 1993;Acher and Chauvet, 1995;Goodson and Bass, 2000). Therefore, the presence of AVT and/or IT receptors (AVTR and ITR, respectively; members of the G-protein coupled receptor family) in specific tissues or organs suggests that these systems are involved in their physiological function (Mahlmann et al, 1994;Hausmann et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Cortisol modulates vasotocinergic and isotocinergic pathways in the gilthead sea bream (Sparus aurata, Linnaeus 1758)

Cádiz

Román-Padilla

Gozdowska

et al. 2014

Journal of Experimental Biology

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In the present study, we assessed the responses of the vasotocinergic and isotocinergic systems to chronic stress induced by cortisol administration in the gilthead sea bream (Sparus aurata). Pituitary and plasma arginine vasotocin (AVT) and isotocin (IT) levels, as well as hypothalamic pro-vasotocin (pro-VT) and pro-isotocin (pro-IT) mRNA expression levels, were analysed. In addition, the mRNA levels of three receptors, AVTR type V1a2, AVTR type V2 and ITR, were analysed in several target organs associated with the following physiological processes: (i) integration and control (hypothalamus), (ii) metabolism and its control (liver and hypothalamus), (iii) osmoregulation (gills) and (iv) stress response (head kidney). Specimens were injected intraperitoneally with slow-release implants (5 μL g −1 body mass) containing coconut oil alone (control group) or with cortisol (50 μg g −1 body mass; cortisol group). Both AVT and IT synthesis and release were correlated with plasma cortisol values, suggesting a potential interaction between both hormonal systems and cortisol administration. Our results suggest that the activation of hepatic metabolism as well as the hypothalamic control of metabolic processes provide the energy necessary to overcome stress, which could be partly mediated by AVTRs and ITR. Upregulation of branchial AVT and IT receptor expression following cortisol treatment suggests an involvement of the vasotocinergic and isotocinergic systems in the regulation of ion channels/transporters during stressful situations. Finally, changes in AVT and IT receptor mRNA expression in the head kidney suggest these nonapeptides participate in feedback mechanisms that regulate the synthesis/release of cortisol. Our results indicate a relationship between cortisol and both the vasotocinergic and isotocinergic systems during simulated chronic stress in S. aurata.

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“…Over the past 20 years, electrophysiological analysis of single cells in the hypothalamus has provided important data on neuronal responses to discrete physical, chemical, and behavioral stimuli (1). Despite these maneuvers, investigators have been unable to identify or directly classify these functionally studied cells chemically.…”

mentioning

confidence: 99%

Intracellular dye-marked enkephalin neurons in the magnocellular preoptic nucleus of the goldfish hypothalamus.

Reaves¹,

Hayward²

1979

Proc. Natl. Acad. Sci. U.S.A.

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A method that combines intracellular recording, dye marking, and immunocytochemistry makes the study of functional and morphological aspects of enkephalin neurons in the magnocellular preoptic nucleus of the goldfish hypothalamus feasible. By use of multiple techniques, enkephalin neurons can be distinguished from other brain cells and can be reconstructed from drawings of serial sections containing the dye-injected opioid cells. These enkephalin cells and their processes measure 14-42,um provided important data on neuronal responses to discrete physical, chemical, and behavioral stimuli (1). Despite these maneuvers, investigators have been unable to identify or directly classify these functionally studied cells chemically. The recent discovery of enkephalin in the magnocellular nuclei, the hypothalamus, and the pituitary gland of the goldfish (2-5) confirms earlier studies in mammals (6-10) and suggests a role for endogenous opioid peptides in neurohypophyseal regulation in fish and mammals. The combination of cell identification by immunocytochemistry with a previously developed intracellular dye-injecting approach in the goldfish (11) provides a working model for chemical typing of single cells. This investigation offers an approach to the analysis of neural networks by use of the current technology of cell identification by dye marking (12), peptide biochemistry (13), and immunocytochemistry (6) for the study of single, electrophysiologically defined, morphologically reconstructed magnocellular neurons that contain enkephalin. MATERIALS AND METHODSAdult goldfish (Carassius auratus), weighing 290-850 g and measuring 25-35 cm, were anesthetized, immobilized, suspended, and perfused with water, and the brain was exposed according to earlier methods (11,14).Glass micropipettes were filled with a 3% aqueous solution of Lucifer Yellow-CH (LY; see ref. 12) and beveled (15) to impedances of 6-40 MQ for intracellular recordings. Electrophysiological recording and stimulating apparatus and the pituitary stimulating electrodes have been described (11). Intracellular electrophoretic injection (5-10 nA of hyperpolarizing dc current, 0.3-6 min) of LY (an anionic fluorescent dye) marked the physiologically studied, antidromically identified preoptic neurons for subsequent localization. In most fish, only one cell per nucleus was filled with LY. At the conclusion of each experiment, the fish was perfused with teleost physiological solution (16) The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.

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Immunocytochemical localization of vasotocin and isotocin in the preopticohypophysial neurosecretory system of teleosts

Cited by 105 publications

References 9 publications

The zebrafish bHLH PAS transcriptional regulator, single‐minded 1 (sim1), is required for isotocin cell development

The zebrafish bHLH PAS transcriptional regulator, single‐minded 1 (sim1), is required for isotocin cell development

Cortisol modulates vasotocinergic and isotocinergic pathways in the gilthead sea bream (Sparus aurata, Linnaeus 1758)

Intracellular dye-marked enkephalin neurons in the magnocellular preoptic nucleus of the goldfish hypothalamus.

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