Immunocytochemical localization of vitamin D-dependent calcium-binding protein in renal tubules of rabbit, rat, and chick

Abstract

Vitamin D-dependent calcium-binding protein (CaBP) was localized in tissue sections of kidneys from rabbits, rats, and chicks using antiserum specific for chick intestinal CaBP. In rabbit kidney, CaBP was present in all cells of the distal convoluted tubule and most cells of the connecting tubule. Fewer, but still a majority, of the cells of cortical collecting ducts contained CaBP. The intensity of immunochemical staining and the number of stained cells decreased markedly in medullary collecting ducts, and only a few collecting duct cells contained CaBP at the junction of the inner and outer medulla. In the rat kidney, CaBP was present in all distal convoluted tubule cells, but the immunochemical staining was less intense than in the rabbit. The protein also was found in most connecting tubule cells of the rat; however, only a few collecting duct cells in the superficial corte of the rat contained CaBP. CaBP was essentially absent from mid- to deep-cortical collecting duct cells, while a very few collecting duct cells always contained CaBP at the junction of the inner and outer stripes of the outer medulla. In the chick, CaBP was present in distal convoluted tubule cells as the distal convoluted tubule coursed adjacent to the central vein. CaBP was absent from chick collecting duct cells. In all three species CaBP was not detected in the other portions of the nephron.

Control of calbindin‐D28K expression in developing mouse kidney

“…The functional reasons for this difference remain obscure. Constitutive expression throughout the embryonic collecting duct tree is quite different from the cortically restricted, 1,25(0H),D3-dependent pattern reported in the adult (Taylor et al, 1982;Rhoten and Christakos, 1981;Roth et al, 1982). It is, however, analogous to the constitutive expression in the drainage channel of embryonic mesonephros, the Wolffian duct.…”

Immunohistochemical Identification of Type B Intercalated Cells in the Rat Kidney by a Monoclonal Antibody.

“…To define the distribution of the OS-3-immunoreactive cells, double immunostaining with calbindin and cytokeratin antisera was performed. In the distal convoluted tubule, all epithelial cells were stained positively by the calbindin antiserum as reported previously (TAYLOR et al, 1982), whereas no cells in this region were immunoreactive with OS-3. In the connecting segment, about two-thirds of the epithelial cells were immunoreactive for calbindin and intermingled with OS-3-immunoreactive cells (Fig.…”

“…Immunohistochemistry for calbindin has labeled almost all epithelial cells in the distal convoluted tubule and also scattered cells in the connecting segment, as previously reported (TAYLOR et al, 1982). Thus, calbindin is regarded as a marker for the distal convoluted tubule and ontogenetically related cells in the connecting segment.…”

Vasopressin-stimulated Ca 2+ reabsorption in rabbit cortical collecting system: effects on cAMP and cytosolic Ca 2+

“…Taylor et al [34], using sections of rabbit kidney, have demonstrated that most cells of CNT and initial portion of the CCD contain calbindin-Dm , which is generally accepted to be indicative of the occurrence of transcellular Ca2* transport. Similar ly, we have demonstrated that the majority («80%) of the cultured cells is calbindin-D28k positive, whereas the re mainder is peanut-lectin positive and, therefore, recog nized as intercalated cells [22,35].…”