Immunocytochemical Study of the Gastroenteropancreatic Endocrine Cells of the Sheep

Calingasan, Noel Y.; Kitamura, Nobuo; Yamada, Junzo; Oomori, Yukio; Yamashita, T

doi:10.1159/000145840

Cited by 56 publications

(27 citation statements)

References 19 publications

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“…The minimal dose of CCK required for stimulation of duodenal motility can be estimated not only from its concentration in the blood but also from the CCK content within the duodenal tissue, which may roughly reflect its plasma concentration assuming that the greater amounts of CCK is released from the duodenal mucosa (Calingasan et al, 1984). The normal plasma level of CCK in most mammals, including sheep, ranges from within 1 to 10 pM Zavros and Shulkes, 1997), which equals 1.14 to 11.4 pg/l.…”

Section: Discussionmentioning

confidence: 99%

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Cholecystokinin-dependent selective inhibitory effect on ‘minute rhythm’ in the ovine small intestine

Romanski

2009

Animal

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Cholecystokinin (CCK) can exert multiple actions on intestinal motility but its effect on the small-intestinal 'minute rhythm' (MR) is virtually unknown. Therefore, the electrical activity from the abomasal antrum, duodenal bulb, duodenum, jejunum and ileum was continuously recorded in six sheep before, during and after slow intravenous administration, of three doses each, of cholecystokinin-octapeptide (CCK-OP) and cerulein. In four of these sheep, two additional electrodes and the strain gauge force transducer were also inserted in the duodenum. Chronic experiments were performed in the fasted and non-fasted animals and saline or CCK peptides were injected during phases 1, 2a or 2b of the duodenal migrating myoelectric complex (MMC). The administration of both CCK peptides in various doses evoked an inhibitory effect mostly in the duodenal bulb, except for the lowest dose of cerulein. The effects of 20 times greater doses of CCK-OP than that of cerulein were more pronounced. The introduction of both CCK peptides during phase 1 of the MMC produced no marked or significant response. In non-fasted animals, the effects of both hormonal peptides, given during phase 2b of the MMC, were often stronger than those given during phase 2a, while in fasted animals the effects of CCK peptides, administered in the course of phases 2a and 2b of the MMC, were similar. Both higher doses of CCK peptides increased the number of spike bursts within the given MR pattern in the duodenum and decreased the incidence of MR mostly in the duodenal bulb. The inhibitory effects of both CCK peptides on the bulbar MR exhibited a dose-response character, though the lowest dose often evoked the slight stimulatory response. It is concluded that CCK principally exerts an inhibitory effect upon the MR in the duodenal bulb and modifies the MR in the duodenum by increasing the spike burst number in a given MR pattern. Both these actions of CCK peptides seem to be physiological. There is a positive relationship between the intensity of the refractory period and the demonstrated effect of CCK in the duodenum.

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Section: Discussionmentioning

confidence: 99%

“…In ruminants, these questions are even less known than in monogastric species. CCK-immunoreactive cells are present in ruminant small-bowel mucosa (Calingasan et al, 1984). In sheep tissues, as in other species, the cholecystokinin-octapeptide (CCK-OP) is present and seems to be one of the major forms Varga et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Cholecystokinin-dependent selective inhibitory effect on ‘minute rhythm’ in the ovine small intestine

Romanski

2009

Animal

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“…Negative control was carried out by incubating sections with the diluent and normal serum instead of the primary antiserum (see Fig. 2B) and positive control was conducted with sections of the ruminant which is previously confirmed [1,2,4,17].…”

Section: Animalsmentioning

confidence: 99%

An Immunohistochemical Study of Endocrine Cells in the Abomasum of Vagotomized Calf.

Soehartono

Kitamura

Yamagishi

et al. 2002

The Journal of Veterinary Medical Science

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ABSTRACT. The effect of thoraco-vagotomy on the distribution and frequency of chromogranin-, serotonin-, somatostatin-and gastrinimmunoreactive cells in the abomasum of the calf were investigated by immunohistochemistry. Calves were vagotomized at 1 week old and sampled 2 and 4 weeks later. The endocrine cells generally decreased in number in vagotomized calves as compared to non-operated control calves. However, the detailed responses of endocrine cells to vagotomy varied depending on the endocrine cell type, region of gastric mucosa, and period after vagotomy. The present result suggests that the vagus nerve has an influence on the intrinsic regulatory system by endocrine cell control in the ruminant abomasum.

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“…In vivo, neuroendocrine forms a completely integrated regulating network, which plays an important role in the organism's health and defense capability for animals (Calingasam et al, 1984). In contrast, the yak gastric mucosa is an ideal organ to study neuroendocrineimmune network due to containing a large number of lymphocytes and a variety of endocrine cells.…”

Section: Discussionmentioning

confidence: 99%

Histomorphometric Adaptation of Yak (Bos grunniens) Abomasum to the Qinghai-Tibetan Plateau Environment

Wang

Zhang

et al. 2015

Int. J. Morphol.

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SUMMARY: Six abomasums of yaks (Bos grunniens) were studied with gross dissection and histological methods. It was found that the mucosa of the yak abomasum was covered with simple columnar epithelium. There were lots of spiral folds (≥10) in the fundic glandular area. The developed membrane of lamina propria was occupied by high density glands. According to the morphological characteristics of the glands, the abomasum was divided into the cardiac, gastric and pyloric glands. Cardiac glands were curved tubular glands with the intumescent bottom and small glandular cavity. Fundic glands were simple tubular glands or branched tubular glands, where the chief, parietal and mucous neck cells can be observed clearly. Pyloric glands were curled tubular glands, the closer to the deep of the lamina propria, the more obvious the glands curl. Staining of glycoconjugate revealed that the mucosal epithelium of the cardiac gastric and pyloric glands and gastric pits epithelium mainly secreted neutral glycoconjugate, but other portions of cardiac and gastric glands secreted mixed and acid glycoconjugate respectively. By Gordon-Sweet's reticular fiber staining, it was found that the mucous neck cells possessed the characteristic of argyrophilic phenomenon. There was a large number of argyrophilic granules in the supranuclear cytoplasm in contrast with the chief cells. Furthermore, there were isolated lymphoid nodules and diffuse lymphoid tissue in the abomasum glands, especially in corpus abomasi. Grimelius silver staining showed that the argyrophil cells were located in the glandular epithelium and lamina propria of glands, which can also be observed in connective tissue. These endocrine cells dispersed individually in epithelial cells, occasionally in 3-5 cell groups. Therefore, the yaks were grazed throughout the year on diverse natural grassland and had evolved morphological characteristics of the abomasum enabling them to consume a wide variety of plant species, thereby better adapting them to harsh plateau environment.

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Immunocytochemical Study of the Gastroenteropancreatic Endocrine Cells of the Sheep

Cited by 56 publications

References 19 publications

Cholecystokinin-dependent selective inhibitory effect on ‘minute rhythm’ in the ovine small intestine

Cholecystokinin-dependent selective inhibitory effect on ‘minute rhythm’ in the ovine small intestine

An Immunohistochemical Study of Endocrine Cells in the Abomasum of Vagotomized Calf.

Histomorphometric Adaptation of Yak (Bos grunniens) Abomasum to the Qinghai-Tibetan Plateau Environment

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