Immunocytochemical study of the glucocorticoid receptor in rat liver nuclei after hyperthermic stress

Čvoro, Aleksandra

doi:10.1016/s1065-6995(03)00019-2

Cited by 6 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found GR nuclear translocation in spiral ganglion neurons after restraint-induced corticosterone elevation, and suppression of this effect when GR was blocked with RU486 + MET. Similar findings have been observed in organs other than the cochlea [14][15][16]. The immediate effects of restraint stress induced a change in GR nuclear translocation and its transcriptional activity without a change in the total GR protein expression.…”

Section: Discussionsupporting

confidence: 84%

Restraint stress modulates glucocorticoid receptors and nuclear factor kappa B in the cochlea

Tahera¹,

Meltser²,

Johansson³

et al. 2006

NeuroReport

View full text Add to dashboard Cite

The regulation of glucocorticoid receptors and nuclear factor kappaB was evaluated in the spiral ganglion neurons after 4 h of restraint stress in the mouse cochlea. Immediately after restraint stress, glucocorticoid receptor protein expression was not altered in spiral ganglion neurons even though both the plasma corticosterone levels and glucocorticoid receptor nuclear translocation increased. By 24 h after restraint stress, the protein expression of glucocorticoid receptors was decreased in spiral ganglion neurons. Pre-treatment with RU486 and metyrapone prevented nuclear translocation of glucocorticoid receptors and nuclear factor kappaB. Moreover, the synthesis of nuclear factor kappaB protein (p65) and inhibitory factor kappaBalpha decreased when RU486 and metyrapone treatment was given before restraint stress. These findings suggest that restraint stress modulates glucocorticoid receptor and nuclear factor kappaB activity in the spiral ganglion neurons, resulting in an altered response to stress.

show abstract

Section: Discussionsupporting

confidence: 84%

Restraint stress modulates glucocorticoid receptors and nuclear factor kappa B in the cochlea

Tahera¹,

Meltser²,

Johansson³

et al. 2006

NeuroReport

View full text Add to dashboard Cite

show abstract

“…They are effective in a variety of diseases including ketosis, allergic reactions, pregnancy toxemia, shock and mastitis. 2,3 However, the mechanisms regulating these effects are not fully understood. The determination of glucocorticoids in different human fluids, such as urine, is of great interest in medical diagnostics, especially for patients suffering from Parkinson's disease or phaeochromocytoma and stress.…”

Section: Introductionmentioning

confidence: 99%

Determination of glucocorticoids using cosurfactants ultrasonic-thermostatic-assisted cloud point extraction followed by high performance liquid chromatography

Qian¹,

Li²,

Chen³

et al. 2012

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Foi desenvolvida uma nova abordagem de extração no ponto nuvem assistida por cossurfactantes ultrassônicos-termostáticos (CUS-CPE), combinada à cromatografia líquida de alta eficiência e detecção por ultavioleta (CLAE-UV), para a análise de glicocorticoides (dipropionato de beclometasona (BD), butirato de hidrocortisona (HB) e fenilpropionato de nandrolona (NPP)) em amostras de urina humana. Neste estudo, quatro sistemas de extração diferentes no ponto nuvem (CPE) são discutidos, incluindo ácido nonanoico e o clássico sulfato de Triton X-100. Entre eles, sulfato de sódio DC-193 e Triton X-100 têm sido estudados nos últimos anos. Comparando com os dois primeiros sistemas, o ácido nonanoico DC-193 teve um ponto nuvem (CP) mais baixo, pouca absorbância UV e provocou danos menores à coluna de três glicocorticoides na mesma concentração de agente tensoativo, necessária como processo de pré-concentração, prévio à CLAE. Diagramas de fase foram usados para estudar os comportamentos de cavitação e transferência de massa das duas fases em micelas de agente tensoativo de silicone orgânico do tipo poliéter, dimeticona . Os volumes da fase rica em agentes tensoativos obtidos foram muito pequenos (o fator de enriquecimento (EF) foi 35), o qual é muito menor e apresenta uma fase de separação mais rápida do que para Triton X-100 na mesma concentração de agente tensoativo. A linearidade foi investigada entre 1 e 350 ng mL -1 . Os limites de detecção (LOD) estimados foram 1,29 para BD, 2,67 para HB e 3,33 ng mL -1 para NPP, respectivamente. O sistema proposto é rápido e eficiente, com recuperação dos três glicocorticoides superior a 85%, o que é similar ou melhor do que os dados relatados na literatura. O método mostrou-se seletivo, linear, preciso e reprodutível, tendo sido aplicado com sucesso na análise de glicocorticoides em amostras de urina humana.A novel approach, cosurfactants ultrasonic-thermostatic-assisted cloud point extraction (CUS-CPE) combined with high performance liquid chromatography and ultraviolet detection (HPLC-UV) is developed for the analysis of glucocorticoids (beclometasone dipropionate (BD), hydrocortisone butyrate (HB) and nandrolone phenylpropionate (NPP)) in human urine samples. In this study, four different cloud point extraction (CPE) systems are discussed, including acid and the classic Triton X-100 sulfate systems. Among them, DC-193-sodium sulfate and the classic Triton X-100 sulfate systems has been studied in the past few years. Comparing with the first two systems, DC-193-nonanoic acid system had a lower cloud point (CP), little UV absorbance and it is less damaging to the column of three glucocorticoids in same surfactant concentration which was required for application as a pre-concentration process prior to HPLC. Phase diagrams were used to study the cavitation and mass transfer behaviors of the two phases on micelles of polyether type organic silicon surfactant, PEG-12 dimethicone .The volumes of surfactant-rich phase obtained were very small (the enrichment factor (EF) was 35), which w...

show abstract

Development and validation of a liquid chromatography–electrosprayionization mass spectrometric method for the determination of dexamethasone in sheep plasma

Panderi

Gerakis²,

Zonaras

et al. 2004

Analytica Chimica Acta

View full text Add to dashboard Cite

Immunocytochemical study of the glucocorticoid receptor in rat liver nuclei after hyperthermic stress

Cited by 6 publications

References 15 publications

Restraint stress modulates glucocorticoid receptors and nuclear factor kappa B in the cochlea

Restraint stress modulates glucocorticoid receptors and nuclear factor kappa B in the cochlea

Determination of glucocorticoids using cosurfactants ultrasonic-thermostatic-assisted cloud point extraction followed by high performance liquid chromatography

Development and validation of a liquid chromatography–electrosprayionization mass spectrometric method for the determination of dexamethasone in sheep plasma

Contact Info

Product

Resources

About