Immunodetection of rainbow trout IL-8 cleaved-peptide: Tissue bioavailability and potential antibacterial activity in a bacterial infection context

Sáenz-Martínez, Daniel E.; Santana, Paula A.; Aróstica, Mónica; Forero, Juan Carlos; Guzmán, Fanny; Mercado, Luís

doi:10.1016/j.dci.2021.104182

Cited by 8 publications

(3 citation statements)

References 59 publications

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“…These results are similar to those found in other fish, where the derived peptide from the end of the Cterminal IL-8 protein was also determined via antibacterial assay, due to generating IL-8 by cutting at the N-terminus leading to more biologically active forms. Sáenz-Martínez et al (2021) [67] reported that 10 µM OmIL-8α 80-97 synthetic peptide from rainbow trout produced a 50% inhibition of bacterial growth in all the tested bacterial strains, including Y. ruckeri, A. salmonicida, Pseudomonas aeruginosa, Staphylococcus aureus and E. coli. Additionally, the synthetic peptide fragment WS12 of snakehead murrel (Channa striata) IL-8 at concentrations between 3.125 and 50 µM has been demonstrated antibacterial activity against Bacillus cereus and E. coli [68].…”

Section: Discussionmentioning

confidence: 99%

Identification, Expression and Antimicrobial Functional Analysis of Interleukin-8 (IL-8) in Response to Streptococcus iniae and Flavobacterium covae in Asian Seabass (Lates calcarifer Bloch, 1790)

Muangrerk,

Uchuwittayakul,

Srisapoome

2024

Animals

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In this research, the proinflammatory cytokine interleukin-8 (IL-8) was shown to play a key role in inflammatory responses in fish. This study involved the cloning of the gene that encodes IL-8 in Asian seabass (Lates calcarifer) as well as analyses of its expression and function in this fish. The expression levels of LcIL-8 indicated that it was broadly expressed in most analyzed tissues, with the most predominant expression in the whole blood 6 to 24 h after infection with S. iniae at concentrations of 105 colony-forming units (CFU)/fish (p < 0.05). After fish were immersed in F. covae, the LcIL-8 transcript was upregulated in the gills, liver and intestine, and the highest expression level was observed in the gills. However, LcIL-8 was downregulated in all the tested tissues at 48 and 96 h after infection with the two pathogenic strains, indicating that Lc-IL8 has a short half-life during the early immune responses to pathogens. Moreover, the MIC of the rLcIL-8 protein against S. iniae was 10.42 ± 3.61 µg/mL. Furthermore, functional analyses clearly demonstrated that 10 and 100 µg of the rLcIL-8 protein efficiently enhanced the phagocytic activity of Asian seabass phagocytes in vitro (p < 0.05). Additionally, in vivo injection of S. iniae following the rLcIL-8 protein indicated that 50 and 100 µg of rLc-IL-8 were highly effective in protecting fish from this pathogen (p < 0.001). The obtained results demonstrate that rLcIL-8 possesses a biological function in the defense against bacterial infections in Asian seabass.

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Section: Discussionmentioning

confidence: 99%

Identification, Expression and Antimicrobial Functional Analysis of Interleukin-8 (IL-8) in Response to Streptococcus iniae and Flavobacterium covae in Asian Seabass (Lates calcarifer Bloch, 1790)

Muangrerk,

Uchuwittayakul,

Srisapoome

2024

Animals

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“…These results are similar to those found in other fish, where the fragment derived from the C-terminal end of IL-8 was also determined by antibacterial assay, due to generating IL-8 by cutting at the N-terminus leading to more biologically active forms. Sáenz-Martínez et al (2021) [63] reported that 10 µM OmIL-8α80-97 synthetic peptide from rainbow trout produced a 50% inhibition of bacterial growth in all tested bacterial strains, including A. salmonicida, Y. ruckeri, Pseudomonas aeruginosa, E. coli and Staphylococcus aureus. Additionally, the IL-8-derived peptide WS12 of snakehead murrel has been demonstrated to possess antibacterial activity against E. coli and Bacillus cereus at concentrations between 3.125 and 50 µM [64].…”

Section: Discussionmentioning

confidence: 99%

Identification, Expression and Antimicrobial Functional Analysis of Interleukin-8 (IL-8) in Response to <em>Streptococcus iniae</em> and Flavobacterium Covae in Asian Seabass (Lates calcarifer Bloch, 1790)

Muangrerk,

Bunnoy,

Srisapoome

2023

Preprint

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Using qRT-PCR analysis, the LcIL-8 expression level indicated broad expression in most detected tissues, with the most predominant expression in whole blood at 6 to 24 h after infection with S. iniae at concentrations of 105 colony-forming unit (CFU)/fish (p &lt; 0.05). After immersion with F. covae, the LcIL-8 transcript was upregulated in the gills, liver and intestine, with the highest expression level found in the gills. However, LcIL-8 was downregulated in all tested tissues at 48 and 96 h after infection with the two pathogenic strains, indicating that Lc-IL8 has a short half-life during the early immune responses to pathogens. MIC analysis of rLcIL-8 protein against S. iniae was 10.42 ± 3.61 µg/mL. Furthermore, the functional analyses clearly demonstrated that 10 and 100 µg of rLcIL-8 protein efficiently enhanced the phagocytic activity of Asian seabass phagocytes in vitro (p &lt; 0.05). Additionally, in vivo injection of S. iniae following the rLcIL-8 protein indicated that 50 and 100 µg of rLc-IL-8 were highly effective in protecting fish from the tested pathogen (p &lt; 0.001). These results indicate that rLcIL-8 plays a crucial role in the defense mechanisms against bacterial infections in the target fish.

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“…IL-8 is a chemokine that regulates the neutrophil activation and transcription of pro-inflammatory cytokines [ 12 ]. In addition, the immunodetection and potential antimicrobial activity of IL-8 has been shown in rainbow trout [ 13 ]. IL-12 is a key component for the efficient performance of phagocytes in teleost fish.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Efficacy of Purified Quillaja Saponin Extracts in Protecting against Piscirickettsia salmonis Infections in Atlantic Salmon (Salmo salar)

Cortés,

Castillo-Ruiz,

Cañon-Jones

et al. 2023

Animals

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Piscirickettsiosis, the main infectious disease affecting salmon farming in Chile, still has no efficient control measures. Piscirickettsia salmonis is a facultative intracellular bacterium that can survive and replicate within the host macrophages, evading the immune response. Triterpenic saponins obtained from the Quillaja saponaria tree have been widely studied, and have been shown to be immunomodulatory agents, suitable for feed and vaccine applications for veterinary and human uses. The impact of the oral administration of two extracts of Quillaja saponins on the infection of P. salmonis in Salmo salar and the corresponding gene expressions of immunomarkers were studied under three in vivo models. In the intraperitoneal challenge model, the group fed with Quillaja extracts showed lower mortality (29.1% treated vs. 37.5% control). Similar results were obtained in the cohabitation model trial (36.3% vs. 60.0%). In the commercial pilot trial, the results showed a significant reduction of 71.3% in mortality caused by P. salmonis (0.51% vs. 1.78%) and antibiotic use (reduction of 66.6% compared to untreated control). Also, Quillaja extracts significantly modulated the expression of IFN-II and CD8. These results represent evidence supporting the future use of purified Quillaja extracts as a natural non-pharmacological strategy for the prevention and control of P. salmonis infections in salmon.

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Immunodetection of rainbow trout IL-8 cleaved-peptide: Tissue bioavailability and potential antibacterial activity in a bacterial infection context

Cited by 8 publications

References 59 publications

Identification, Expression and Antimicrobial Functional Analysis of Interleukin-8 (IL-8) in Response to Streptococcus iniae and Flavobacterium covae in Asian Seabass (Lates calcarifer Bloch, 1790)

Identification, Expression and Antimicrobial Functional Analysis of Interleukin-8 (IL-8) in Response to Streptococcus iniae and Flavobacterium covae in Asian Seabass (Lates calcarifer Bloch, 1790)

Identification, Expression and Antimicrobial Functional Analysis of Interleukin-8 (IL-8) in Response to <em>Streptococcus iniae</em> and Flavobacterium Covae in Asian Seabass (Lates calcarifer Bloch, 1790)

In Vivo Efficacy of Purified Quillaja Saponin Extracts in Protecting against Piscirickettsia salmonis Infections in Atlantic Salmon (Salmo salar)

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