Immunodosages et détection infrarouge

In infrared immunoassay, organometallic markers containing metal-carbonyl moieties are employed in conjunction with FTIR spectroscopy as a detection method. These metal-carbonyl markers have characteristic and intense absorption bands in a region of the infrared spectrum (2200-1800 cm') that is devoid of other strong absorptions and can be detected at picomole levels. In addition, the ability to individually detect different metal-carbonyl markers in the presence of each other affords the possibility of determining multiple antigens in a single test. In the present work, we have undertaken the simplification and automation of the infrared immunoassay method in order to increase its potential utility for routine use. A solid-phase infrared iminimoassay test has been developed in which antibodies specific to the analytes of interest are adsorbed on the surface of an W-transparent membrane. In an alternative homogeneous immunoassay format, antigens are adsorbed on the surface of an internal reflection element (IRE), and the binding of these antigens to the corresponding antibodies in a solution placed in contact with the IRE is monitored by attenuated total reflectance spectroscopy. Competition between the adsorbed antigens and free antigens in the solution for the antibody binding sites then provides the basis for a homogeneous immunoassay.

show abstract

<title>Clinical infrared spectroscopy: multiple antigen determination by solid-phase infrared immunoassay and homogeneous infrared immunoassay</title>

Ismail

Barnett

1998

SPIE Proceedings

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Immunodosages et détection infrarouge

Cited by 1 publication

References 7 publications

<title>Clinical infrared spectroscopy: multiple antigen determination by solid-phase infrared immunoassay and homogeneous infrared immunoassay</title>

<title>Clinical infrared spectroscopy: multiple antigen determination by solid-phase infrared immunoassay and homogeneous infrared immunoassay</title>

Contact Info

Product

Resources

About