Immunofluorescent serum gliadin antibodies in children with coeliac disease and various malabsorptive disorders

Abstract

An immunofluorescent gliadin antibody assay is described using pyruvic aldehyde-stabilized human erythrocytes coated with gliadin. Fifty coeliac children all had high serum IgG-antigliadin titres during a normal diet or a challenge with gluten. On a gluten-free diet (30 children), titres were much lower. In patients followed-up for one year on a gluten-free diet, an initial rise in titres was followed by a slow decline. On challenge, IgG-antigliadin titres showed a slow rise or persistence at the same level in most patients. Fifty-two percent of control children with malabosrptive disorders, but without the typical "flat" mucosal lesion on jejunal biopsy, were shown to have positive titres in their sera, as were 6% of normal children and 4% of adult blood donors. The fluorescent antibody technique was compared with methods commonly used to detect wheat-protein antibodies, and was found to be superior to all of them. The immunofluorescent gliadin antibody assay appears to be useful in following-up children with coeliac disease, and in selecting patients for jejunal biopsy, although it does not replace biopsy.

Genetic difference in HLA-DR phenotypes between coeliac disease and transitory gluten intolerance.

“…Production of antigliadin antibodies of the IgG class and IgA class as well as antiendomysial antibodies have been shown to correlate with the immune response to gliadin. [16][17][18][19] In the mouse, two separate genetic loci on chromosomes 12 and 17 control the immune response to gliadin. '2 This study was performed to investigate whether there are genetic differences in the HLA-DR class antigens in children with coeliac disease and those with transitory gluten intolerance.…”

Serologic Markers of Untreated Celiac Disease in Libyan Children: Antigliadin, Antitransglutaminase, Antiendomysial, and Anticalreticulin Antibodies

“…Different serologic tests (antigliadin, antitissue transglutaminase, anticalreticulin and antiendomysial antibodies) are commonly used for screening of celiac disease (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32). These tests at present are not available in Libya.…”

Coeliac Disease. Pathogenesis and clinical aspects

“…Therefore, measurement of gliadin antibodies was suggested as a screening test and as a diagnostic tool for the disease (Burgin-Wofff2983). In 1983, investigations on gliadin antibody specificity had not revealed evidence of primary pathogenetic significance of the circulating gliadin antibodies (Stern 1979, but it was regarded as apparent that there was an association between coeliac disease and the systemic immune response to gliadin.…”