Immunofluorescent Staining of Mucosal Glycoprotein of Human Intestinal Mucosa

Kawasaki, Hiroshi; Imasato, Katsujiro; Nakayama, Kagechika; Akiyama, Kanji; Kimoto, Eiji; Takeuchi, Mutsuya

doi:10.1267/ahc.5.26

Cited by 9 publications

(1 citation statement)

References 8 publications

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“…Hard clay, also known as Kaolin, containing Si–O and Si–OH groups on its surface, are known to act as low reinforcing fillers in rubbers 1–3. The reinforcing ability of clay is poor in comparison to precipitated silica and carbon blacks, although there has been a report on the use of clays in tire compounds 4.…”

Section: Introductionmentioning

confidence: 99%

Bonding between epoxidized natural rubber and clay in presence of silane coupling agent

Manna

Tripathy

et al. 1999

J. Appl. Polym. Sci.

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Based on the results of bound-rubber determination, Monsanto rheometric studies, solvent swelling, measurement of physical properties, and infrared spectroscopic studies, it is revealed that epoxidized natural rubber (ENR) and hard clay interact chemically to form Si-O-C bond during high-temperature (180°C) molding. It is also observed that addition of the silane coupling agent N-3-(N-vinyl benzyl amino)ethyl-␥-amino propyl trimethoxy silane monohydrogen chloride enhances the extent of the chemical interaction with the formation of coupling bonds of Si-O-Si type between clay and the coupling agent and C-N bonds between ENR and the coupling agent.

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Section: Introductionmentioning

confidence: 99%

Bonding between epoxidized natural rubber and clay in presence of silane coupling agent

Manna

Tripathy

et al. 1999

J. Appl. Polym. Sci.

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Purification of human intestinal goblet cell antigen (GOA1), its immunohistological demonstration in the intestine and in mucus producing gastrointestinal adenocarcinomas

Rapp

Windisch

Peschke

et al. 1979

Virchows Arch. A Path. Anat. and Histol.

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Goblet cell antigen (GOA) was purified from gastric signet ring cell carcinoma. It was immunogenic and was used to produced antisera which stained goblet cells of the small and large intestine and of intestinalized gastric mucosa by indirect immunological methods. Various types of gastric and colonic cancer contained GOA. These findings demonstrate a histiogenic relationship between intestinal goblet cells, various gastrointestinal cancers and associated premalignant conditions.

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Histochemical localization of estrogen induced sulfated glycoprotein in rabbit uterus

et al. 1976

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Histochemical localization of the estrogen-induced sulfated glycoproteins was made in the estrogen-treated rabbit uterus. Biochemical studies by a group of Endo et al, affirmed these particular glycoproteins were PAS-positive and metachromatic as stained with TB. No sign of digestion, however, has been detected in a series of tests with alpha-amylase, testicular hyaluronidase, streptomyces hyaluronidase, chondroitinase AC and chondroitinase ABC, and heparinase. The apical portions of the epithelial and glandular cells, obviously expanded by the estrogen treatment, display strong beta-metachromasia with TB (pH 4.0), saliva-resistant PAS-positive reactions, and also alcianophilia with AB (pH 2.5). These reactions are not reduced after the treatment with the enzymes above-mentioned. Meanwhile, in the stromal matrix, the same enzymes give an influence to diminish the reactions to various extent. Our results suggest that the estrogen-induced sulfated glycoprotein is definitely localized in the apical portions of the epithelial and glandular cells. The identity is emphasized between the substance that is elucidated in the histochemical sections and the sulfated glycoproteins that have been specified solely by means of biochemical assays.

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Immunofluorescent Staining of Mucosal Glycoprotein of Human Intestinal Mucosa

Cited by 9 publications

References 8 publications

Bonding between epoxidized natural rubber and clay in presence of silane coupling agent

Bonding between epoxidized natural rubber and clay in presence of silane coupling agent

Purification of human intestinal goblet cell antigen (GOA1), its immunohistological demonstration in the intestine and in mucus producing gastrointestinal adenocarcinomas

Histochemical localization of estrogen induced sulfated glycoprotein in rabbit uterus

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