Immunogenetics of two new HLA alleles: A*0108 and B*4031

Street, J.; Downing, Jonathan; Hammond, Laura; Thompson, John; Darke, C.

doi:10.1034/j.1399-0039.2003.00033.x

Cited by 6 publications

(1 citation statement)

References 14 publications

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“…Novel, rare and unusual HLA phenotypes, e.g. A*0108, B*4031 (Street et al ., 2003b) , B*4408 (Street et al ., 2003a), B*4414, B*5708 (Darke et al ., 2003) , DRB1*0704 and DRB1*1507 (Darke et al ., 2000), were highlighted during our routine clinical testing and from typing of volunteer haematopoietic stem cell donors for the Welsh Bone Marrow Donor Registry (Darke, 2000). Informed, written consent was obtained from donors (or their representatives in the case of cadaveric organ donors) before B‐lymphocytes were transformed into a BCL, for use in routine and research procedures on a strictly non‐commercial basis.…”

Section: Introductionmentioning

confidence: 99%

Provision of Epstein‐Barr virus‐transformed B‐cell lines in a routine tissue typing laboratory: practicalities and applications

Bass

Walters

Darke

2004

European Journal of Immunogenetics

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A bank of Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cell lines (BCLs) has been established in this laboratory over the last 7 years. Novel, rare and unusual HLA phenotypes were highlighted during routine clinical testing and during typing of volunteer haematopoietic stem cell donors for the Welsh Bone Marrow Donor Registry. The BCLs are routinely used as laboratory reagents, as a source of DNA for reference purposes and for research work. Consenting donors are tested for hepatitis B surface antigens (HBsAg), human immunodeficiency virus (HIV) and hepatitis C virus (HCV) before their B-lymphocytes are transformed. Strict bio-identity checks are performed before and after transformation and after each BCL expansion. Each BCL is tested regularly for mycoplasma contamination. A total of 230 blood samples were transformed. One hundred and fifty-nine sterile samples produced 157 BCLs (98.7% success), while 71 non-sterile samples produced 50 BCLs (70.4% success), giving an overall success rate of 90.0%. Fifteen of the transformation failures have since been repeated successfully. Factors contributing to the high success rate and reasons for the 23 failed transformations are discussed. The successful development and use of pools of BCLs for HLA antibody screening by flow cytometry are described. Whilst certain training and health and safety issues require close attention, it is clear that EBV transformation of B lymphocytes and/or the use of BCLs is feasible in a routine tissue typing laboratory and that BCLs are a valuable resource. Careful adherence to the methods and procedures detailed here should virtually guarantee successful transformation (98.7%) from good quality, sterile whole blood samples.

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Section: Introductionmentioning

confidence: 99%

Provision of Epstein‐Barr virus‐transformed B‐cell lines in a routine tissue typing laboratory: practicalities and applications

Bass

Walters

Darke

2004

European Journal of Immunogenetics

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HLA dictionary 2004: Summary of HLA-A, -B, -C, -DRB1/3/4/5, -DQB1 alleles and their association with serologically defined HLA-A, -B, -C, -DR, and -DQ antigens

Schreuder¹,

Hurley

Marsh³

et al. 2005

Human Immunology

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This report presents serologic equivalents of human leukocyte antigen (HLA)-A, -B, -C, -DRB1, -DRB3, -DRB4, -DRB5, and -DQB1 alleles. The dictionary is an update of the one published in 2001. The data summarize equivalents obtained by the World Health Organization (WHO) Nomenclature Committee for Factors of the HLA System, the International Cell Exchange (UCLA), the National Marrow Donor Program, recent publications, and individual laboratories. This latest update of the dictionary is enhanced by the inclusion of results from studies performed during the 13th International Histocompatibility Workshop and from neural network analyses. A summary of the data as recommended serologic equivalents is presented as expert assigned types. The tables include remarks for alleles, which are or may be expressed as antigens with serologic reaction patterns that differ from the well-established HLA specificities. The equivalents provided will be useful in guiding searches for unrelated hematopoietic stem cell donors in which patients and/or potential donors are typed by either serology or DNA-based methods. The serological-DNA equivalent dictionary will also aid in typing and matching procedures for organ transplant programs whose waiting lists of potential donors and recipients are comprised of mixtures of serologic and DNA-based typings. The tables with HLA equivalents and a questionnaire for submission of serologic reaction patterns for poorly identified allelic products will be made available through the World Marrow Donor Association Web page (www.worldmarrow.org).

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Five-Locus HLA Typing of Hematopoietic Stem Cell Donor Volunteers Using PCR Sequence Specific Primers

Downing

Guttridge²,

Thompson³

et al. 2004

Genetic Testing

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We have developed a strategy for five-locus human leukocyte antigen (HLA) typing of hematopoeitic stem cell (HSC) donors using the polymerase chain reaction with sequence-specific primers (PCR-SSP). The PCR-SSP method is robust, reproducible, and accurate. New PCR-SSP mixtures can be added as required and all reactions are carried out under the same conditions, which can easily be applied to the typing of other loci, e.g., ABO blood groups. Initially, 127 PCR-SSP reactions were used to detect simultaneously HLA-A, -B, -C, -DRB1/3/4/5, and DQB1 alleles, differentiated generally to the level of the first two digits of the allele name, essentially equivalent to the serological split specificity. Approximately 40% of subjects were tested against a further 29 HLA-A, -B SSP mixtures to exclude rare alleles and unambiguously assign a two-digit HLA allele family. This gave an overall typing resolution equivalent to or greater than the split specificity level and covered all HLA-A, -B, -C, -DRBland DQB1 alleles listed in the WHO's Nomenclature for Factors of the HLA System, 2000. The Welsh Bone Marrow Donor Registry has used this strategy to HLA type over 35,000 HSC donors over 9 years. Comprehensive and accurate five-locus HLA typing allows confident and rapid identification of potential matched HSC donors for patients requiring stem cell transplantation generally without the need for typing additional loci. This allows resources to be focused directly on allele level typing of DRB1 and other loci. This strategy decreases overall donor work-up time, which is a major benefit to patients.

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Immunogenetics of two new HLA alleles: A0108 and B4031

Cited by 6 publications

References 14 publications

Provision of Epstein‐Barr virus‐transformed B‐cell lines in a routine tissue typing laboratory: practicalities and applications

Provision of Epstein‐Barr virus‐transformed B‐cell lines in a routine tissue typing laboratory: practicalities and applications

HLA dictionary 2004: Summary of HLA-A, -B, -C, -DRB1/3/4/5, -DQB1 alleles and their association with serologically defined HLA-A, -B, -C, -DR, and -DQ antigens

Five-Locus HLA Typing of Hematopoietic Stem Cell Donor Volunteers Using PCR Sequence Specific Primers

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