Immunogenic properties of the protein component of Treponema pallidum.

Metzger, M; Michalska, E; Podwińska, J; Smogór, W

doi:10.1136/sti.45.4.299

Cited by 31 publications

(26 citation statements)

References 8 publications

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“…The results previously reported (Metzger, Michalska, Podwin'ska, and Smog6r, 1969) have thus been corroborated and extended.…”

Section: Discussionsupporting

confidence: 79%

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mentioning

confidence: 68%

See 1 more Smart Citation

Artificial immunization of rabbits against syphilis. I. Effect of increasing doses of treponemes given by the intramuscular route.

Metzger¹,

Smogór²

1969

Sexually Transmitted Infections

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The results reported in a previous paper (Metzger, Michalska, Podwiriska, and Smog6r, 1969) have clearly shown that the immunogenic properties of Treponema pallidum are related to their protein component. A high degree of immunity to challenge inoculation by virulent treponemes was obtained in rabbits that had been injected with killed treponemes with the protein antigen preserved, as judged by their high agglutinability in a serum that contained antibody to this component only. Two vaccine preparations proved very efficient when given intravenously to rabbits:(1) Treponemes maintained without any additions at 4°C. for from 7 to 10 days before administration.(2) Treponemes stored for the same time but with peniciUin added (4 .tg./ml.).The main purpose of the present study was to check the efficiency of these preparations when administered intramuscularly and to investigate the effect of treponeme dose on the development of resistance. Material and MethodsThe virulent Nichols strain of T. pallidum was used throughout.Adult Danish albino rabbits weighing about 3 kg., with non-reactive VDRL and TPI tests, were used for the study; they were housed in individual cages out of doors. VaccinesThree vaccines prepared from killed T. pallida were given as immunizing agents:(1) Treponemes suspended in phosphate-buffered saline of pH 7.4 and stored at 4°C. for from 7 to 10 days. *Received for puiblication April 21, 1969 tThis study was supported by grants from the,World Health Orztion. 308(2) Treponemes suspended in phosphate-buffered saline of pH 7.4 with penicillint added (1 Vg./ml.); stored at 37'C. for 24 hours and at 4°C. for 6 to 9 days.(3) Treponemes suspended in phosphate-buffered saline of pH 7-4 with penicillin added (4 jLg./ml.); stored at 370C. for 24 hours. Non-infectiousness of the vaccines was ascertained by the following experiments:(1) Each new batch of treponeme suspensions was tested for virulence by intracutaneous injection of 0.1 ml. containing 29 million organisms into each of four sites on the shaven back of a rabbit, and by injection of 05 ml. containing 145 million organisms into each testis of the same rabbit. None of the rabbits developed lesions during a 4-month observation period, and lymph node transfers from these rabbits to other rabbits were negative. All the animals remained VDRL and TPI negative.(2) 4 or 5 weeks after the completion of the immunization procedure, the left popliteal lymph node of each animal was removed and emulsified in 1 ml. 10 per cent. normal rabbit serum-saline; one drop of the emulsion was inspected by darkfield microscopy for the presence of treponemes, and the rest was injected into one testis of a normal rabbit; the second testis was utilized for lymph node transfer from another rabbit. Microscopic examination of the testes performed one month later did not show the presence of treponemes in any of the testicles. ImmunizationOnly female rabbits were used. They were injected intramuscularly with various vaccines over a period of 7 weeks. Each rabbit received 1 ml....

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“…The results previously reported (Metzger, Michalska, Podwin'ska, and Smog6r, 1969) have thus been corroborated and extended.…”

Section: Discussionsupporting

confidence: 79%

“…

…”

mentioning

confidence: 68%

Artificial immunization of rabbits against syphilis. I. Effect of increasing doses of treponemes given by the intramuscular route.

Metzger¹,

Smogór²

1969

Sexually Transmitted Infections

Self Cite

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show abstract

“…The results, therefore, of three groups of investigators are of interest, viz. Tani, Inoue, and Asano (1951), Miller (1967, 1973, Metzger and others (1969), and Metzger and Smog6r (1969). Tani and others (1951) studied the effect on protective immunity against T. pallidum in rabbits immunized by carbolic acid saline suspensions of T. pallidum.…”

Section: Discussionmentioning

confidence: 99%

“…A high degree of immunity was also obtained by intramuscular injection of non-viable T. pallidum, the degree of immunity depending on the number of organisms injected during the course of immunization (Metzger and Smog6r, 1969). Thus ten rabbits immunized intramuscularly by T. pallidum with a total dose of 1-2 x 1010 given over a period of 7 wks were challenged 5 wks after completing the immunization by intradermal injection at each of four sites with 300,000 T. pallidum.…”

Section: Discussionmentioning

confidence: 99%

Immunity in syphilis. Studies in active immunity.

Al-Samarrai¹,

Henderson²

1976

Sexually Transmitted Infections

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“…Protection has been demonstrated in the rabbit model by using complicated immunization protocols with gamma-irradiated, antiformin-treated, or 4°C "aged" treponemes (34,35,41). Although these regimens are impractical and not suitable for humans, they do demonstrate that protective immunity by vaccination is attainable.…”

mentioning

confidence: 99%

Immunization with the N-Terminal Portion ofTreponema pallidumRepeat Protein K Attenuates Syphilitic Lesion Development in the Rabbit Model

Morgan¹,

Lukehart

Voorhis

2002

Infect Immun

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When used as an immunogen, Treponema pallidum repeat protein K (TprK) has been shown to attenuate syphilitic lesions upon homologous intradermal challenge in the rabbit model. To further explore this protein as a potential vaccine component, we sought to identify the immunogenic regions of TprK. The abilities of three recombinant peptides encompassing TprK to elicit T-and B-cell responses and to protect against challenge were examined. All three fragments elicited proliferative responses from splenocytes taken from infected rabbits. However, enzyme-linked immunosorbent assays indicated that only fragments 1 and 3 were consistently recognized by antisera from infected rabbits. Each fragment was also used to immunize rabbits that were subsequently challenged intradermally with infectious T. pallidum. All lesions on unimmunized control rabbits ulcerated and contained treponemes, while the lesions on rabbits immunized with fragment 1 were the least likely to have detectable treponemes (25%) and the least likely to ulcerate (37.5%). The lesions on rabbits immunized with fragment 3 showed intermediate results, and rabbits immunized with fragment 2 were the most likely of all those on immunized rabbits to have detectable treponemes (91.7%) and to ulcerate (66.7%). These results demonstrate that epitopes in fragment 1 are recognized by T cells and antibodies during infection and that immunization with this portion of TprK most effectively attenuates syphilitic lesion development.

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Immunogenic properties of the protein component of Treponema pallidum.

Cited by 31 publications

References 8 publications

Artificial immunization of rabbits against syphilis. I. Effect of increasing doses of treponemes given by the intramuscular route.

Artificial immunization of rabbits against syphilis. I. Effect of increasing doses of treponemes given by the intramuscular route.

Immunity in syphilis. Studies in active immunity.

Immunization with the N-Terminal Portion ofTreponema pallidumRepeat Protein K Attenuates Syphilitic Lesion Development in the Rabbit Model

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