Immunogenicity of antigen‐conjugated biodegradable polydiacetylene liposomes administered mucosally

Abstract

In this work, we report a protocol for synthesizing nanosize ovalbumin-functionalized polydiacetylene (PDA) liposomes (LP-Ova). We show that LP-Ova administered per-orally (p.o.) and subcutaneously (s.c.), without the use of adjuvants, induces high serum IgG1 titers. As reported previously using polystyrene nanoparticles (NPs), p.o.-primed mice developed high titers of IgG2c and intestinal IgA following s.c. boosting immunization with LP-Ova. Mice that received a single s.c. immunization with LP-Ova did not develop serum IgG2c or intestinal IgA antibodies. Additionally, in s.c.-immunized mice serum IgG1 titers decreased significantly by 3 months after immunization. In contrast, in mice primed p.o. and boosted s.c. with LP-Ova, serum IgG1/IgG2c, and intestinal IgA antibody titers remained stable. Administration of LPs exerted no adverse effects on immunized mice as no morbidity or signs of toxicity were observed for the duration of the studies. These results indicate that antigen-conjugated liposomes are immunogenic and confirm a previous report that mucosal priming followed by a s.c. boosting immunization is the most effective strategy for inducing long-lasting mucosal IgA, as well as a polarized Th1/Th2 systemic response. In addition to being biodegradable and easily functionalized by conjugation, liposomes have a hollow core which can also be loaded with cargo, allowing for a targeted delivery of multiple antigens (or drugs) simultaneously. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 557-565, 2017.

The Nano–Intestine Interaction: Understanding the Location‐Oriented Effects of Engineered Nanomaterials in the Intestine

“…Moreover, NMs with different modification can also resist the invasion of Salmonella enteritis and prevent anaphylactic shock through modulating immune responses through enhancing Th1 effect, the release of Treg cytokines, and immune memory. [95][96][97][98][99] These studies indicate that NMs either serve as delivery vector for drugs, or as nanotherapeutic agents with intrinsic antiinflam matory properties.…”

Encapsulation of Plant Growth-Promoting Bacterial Crude Extract in Nanoliposome and Its Antifungal Property Against Fusarium oxysporum

“…For extrusion to be effective, usually filtration of solution multiple times (10 times or more) is needed to reduce and control the nanoliposome size (for example, please see Abrams et al). 31 After nanobiofungicide particles were sonicated, they were stored at 4 °C. The storage at low temperature facilitates the self-assembly of the lipids and fatty acids in the membrane.…”

“…This process was not intended for extrusion purpose. For extrusion to be effective, usually filtration of solution multiple times (10 times or more) is needed to reduce and control the nanoliposome size (for example, please see Abrams et al) …”