Immunogenotyping in Hodgkin's disease

Griesser, Henrik; Mak, Tak W.

doi:10.1002/hon.2900060307

Cited by 26 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If the same phenomenon occurs in H D , the occurrence of breakpoints at these sites may help to identify the lineage of the tumor, a subject of intense study in recent years. Since some evidence of both B-cell and T-cell lineage in H D has been found by both immunohistochemical (Stein et al, 1986;Coles et al, 1988;Cibull et al, 1989;Dallenbach and Stein, 1989) and molecular genetic methods (Griesser and Mak, 1988), the occurrence of translocations at the sites of antigen receptor genes would be expected in at least some cases. However, only band 14q32 has been reported to be involved with any frequency.…”

Section: Discussionmentioning

confidence: 99%

Clonal cytogenetic abnormalities in Hodgkin's disease

Ladanyi

Parsa

Offit

et al. 1991

Genes Chromosomes & Cancer

View full text Add to dashboard Cite

Cytogenetic studies of Hodgkin's disease (HD), in contrast to those of non-Hodgkin's lymphoma (NHL), have been limited to small numbers of cases with infrequently recurring aberrations, underscoring the need for additional studies in establishing a coherent cytogenetic picture of HD. Over a 6 1/2-year period, we received 95 specimens of HD for cytogenetic analysis. Analyzable chromosome preparations were obtained in 70 cases, of which 57 (81%) showed only normal metaphases. In the remaining 13 cases (19%), karyotypic abnormalities were observed that were nonclonal in 3 and clonal in 10. The latter 10 cases included 6 of the nodular sclerosis subtype, 3 mixed cellularity, and 1 lymphocyte-depleted; 8 of the specimens were obtained pretreatment and 2 posttreatment. Two of the cases had a clonal numerical aberration, monosomy 17 in one and trisomy 13 in the other, as their sole abnormality. The remaining 8 cases showed complex karyotypes with multiple structural rearrangements; in 3 of these, the abnormal clone was near-tetraploid. Bands involved more than once included 1p36, 1q21, and 4q35, each in 2 cases. Arms involved more than once included 6q (6q13,6q23), 9p (9p13,9p21), and 5q (5q15,5q35). Three patients had loss of part or all of 6q (del(6)(q13),del(6)(q23),i(6p). Bands 14q32 and 18q21 were not involved in any case, contrary to some previous reports. Our results confirm the frequent occurrence of 1p, 1q, and 6q abnormalities in HD. In addition, we have identified a 5q35 breakpoint, which has recently been shown to be highly specific for Ki-1-positive NHL in a case of typical nodular sclerosis HD. Its presence in HD may represent a cytogenetic link between the two entities, which are immunophenotypically related but clinically and histologically distinct.

show abstract

Section: Discussionmentioning

confidence: 99%

Clonal cytogenetic abnormalities in Hodgkin's disease

Ladanyi

Parsa

Offit

et al. 1991

Genes Chromosomes & Cancer

View full text Add to dashboard Cite

show abstract

“…Presumably, the heterogeneous nature of Hodgkin's disease is the reason for the lack of accordance in data concerning the cellular origin of the neoplastic population on the one hand and the karyotype changes on the other. Several molecular genetic studies have demonstrated that H and SR cells show rearrangements and expression specific for either B-cell or for T-cell lineage (Falk et al 1987;Griesser et al 1986a, b;Griesser and Mak 1988;O'Connor et al 1987;Tesch et al 1988). Moreover, the results obtained on established cell lines and on primary Hodgkin's lymphomas suggest that H and SR cells are immature lymphoid cells that are activated by as yet unknown mechanisms (Falk et al 1987;Tesch et al 1988).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal in situ hybridization of a Hodgkin's disease-derived cell line (L540) using DNA probes for TCRA, TCRB, MET, and rRNA

et al. 1990

View full text Add to dashboard Cite

The Hodgkin's lymphoma-derived cell line L540, which exhibits multiple marker chromosomes and a genotype characteristic of T-cell origin, showed expression of MET oncogene. L540 was studied by in situ hybridization for chromosomal rearrangements concerning the regions where genes for TCRA and TCRB chains, for MET oncogene, and for rRNA are located. All four genes were demonstrated to be involved in the formation of marker chromosomes. Especially remarkable was the participation of rDNA-bearing regions in two different translocations.

show abstract

“…Immune receptor gene analyses are of limited value in the differential diagnosis of Hodgkin's disease (HD) versus non-Hodgkin's lymphoma in morphologically equivocal cases. Immunogenotyping sometimes may not help in differential diagnosis between peripheral T cell lymphoma with atypical large T blasts and HD (usually of mixed-cellularity subtype or unclassified): TCRI3 rearrangements have been observed in HD as well [60,75,144]. The separation of HD from non-Hodgkin's lymphoma thus remains impossible at times.…”

Section: Tcr Gene Probesmentioning

confidence: 99%

Gene rearrangements and chromosomal translocations in T cell lymphoma ? diagnostic applications and their limits

Griesser

1995

Vichows Archiv A Pathol Anat

View full text Add to dashboard Cite

The diversity of the T cell receptor (TCR) repertoire is established for individual T lymphocytes by developmentally regulated gene rearrangements and shaped by predominantly intrathymic selection procedures. TCR gene probes in Southern blot experiments and TCR primers for the polymerase chain reaction (PCR) help to distinguish polyclonal from abnormal clonal T cell proliferations and to monitor clonal disease after treatment. Rearrangement studies can identify the lineage and developmental stage of a lymphocyte clone. Cross-lineage rearrangements, false positive or negative results are rarely misleading when morphology and immunophenotypical findings are considered. Rearrangement studies, however, have not contributed significantly to the comprehension of lymphomagenesis. Analyses of characteristic chromosomal translocations in T cell leukaemias and lymphomas may provide further insight into the mechanisms of malignant transformation. Transcription factors are often involved and sometimes abnormally transcribed, which may alter the physiological intracellular signalling in T cells. Interphase cytogenetic analysis by chromosomal fluorescence in situ hybridization (FISH) has become a new tool in the search for transformed T cells carrying specific translocations. Archival biopsy material is now accessible for PCR rearrangement studies and FISH cytogenetics. This adds another dimension to the diagnosis, disease monitoring and biological understanding of malignant T cell lymphomas and leukaemias.

show abstract

Immunogenotyping in Hodgkin's disease

Cited by 26 publications

References 31 publications

Clonal cytogenetic abnormalities in Hodgkin's disease

Clonal cytogenetic abnormalities in Hodgkin's disease

Chromosomal in situ hybridization of a Hodgkin's disease-derived cell line (L540) using DNA probes for TCRA, TCRB, MET, and rRNA

Gene rearrangements and chromosomal translocations in T cell lymphoma ? diagnostic applications and their limits

Contact Info

Product

Resources

About