Immunoglobulin-containing cells in the colonic mucosa of rabbits with carrageenan-induced colitis

Matsumoto, Takayuki; Kitano, Atsuo; Oshitani, Nobuhide; Obata, Akishige; Hiki, Masato; Hashimura, Hidechika; Okawa, Kiyotaka; Nishimura, Hiroshi; Kobayashi, Kenzo

doi:10.1007/bf02552594

Cited by 11 publications

(3 citation statements)

References 25 publications

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“…In this study, the number of IgA-expressing, as a representation of humoral responses in the intestinal mucosa, was examined. Among all immunoglobulins, IgA-producing cells are predominant in number because they play a major role in limiting the penetration of antigens through the mucosal epithelium (17,18).…”

Section: Discussionmentioning

confidence: 99%

Effects of a multispecies synbiotic on intestinal mucosa immune responses

Athiyyah¹,

Widjaja²,

Fitri³

et al. 2019

IJM

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Background and Objectives: Probiotics and prebiotics are known to regulate immune responses. A synbiotic is a product that combines probiotics and prebiotics in a single dosage form. In this study, we attempt to present the effects of a multispecies synbiotic on intestinal mucosa immune responses after exposure to Escherichia coli O55:B5 lipopolysaccharide (LPS). Materials and Methods: Totally 21 male Balb/c mice were randomly classified into two groups. The K-I group received LPS and a synbiotic, and the K-II group received LPS alone. The synbiotic was administered for 21 consecutive days, whereas LPS was administered once on the 15 th day. Specifically, a synbiotic containing 1 × 10 9 colony forming units (CFUs) of the probiotic combination of Lactobacillus acidophilus PXN 35, L. casei subsp. casei PXN 37, L. rhamnosus PXN 54, L. bulgaricus PXN 39, Bifidobacterium breve PXN 25, B. infantis PXN 27 and Streptococcus thermophilus PXN 66 and the prebiotic fructo-oligosaccharide was administered through an orogastric tube. Immunohistochemistry was performed to measure immunoglobulin A (IgA) levels for humoral immune responses and CD4+ and CD8+ levels for cellular immune responses. Results: An independent-samples t-test revealed significant increases of the numbers of IgA-(p = 0.027) and CD4-expressing cells (p = 0.009) but not the number of CD8-expressing cells in the K-I group compared with those in the K-II group. Conclusion: The multispecies synbiotic had immunoregulatory effects on IgA and CD4 expression in LPS-exposed mice.

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Section: Discussionmentioning

confidence: 99%

Effects of a multispecies synbiotic on intestinal mucosa immune responses

Athiyyah¹,

Widjaja²,

Fitri³

et al. 2019

IJM

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“…They were stored at -80 C until use. Serial cryostat sections were prepared and stained by the indirect immunoperoxidase method as previously described [18]. Briefiy.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Multifunctional effects of anticomplementary agent K-76 on carrageenan-induced colitis in the rabbit

Kitano¹,

Matsumoto

Nakamura

et al. 1993

Clinical and Experimental Immunology

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SUMMARYIn this study the effeet of K-76, a sesquitetpene compound with antieomplementary activity isolated from a fungus culture, on carrageenan-indueed eolitis was studied from biochemical, histological and immunohistopathologieal aspects. K-76 suppressed epithelial cell loss, crypt abscess formation, inflatiimatorycell infiltration, mucosal atrophy, and ulceration. Imniunohistoehemical examination ofthe colonic mucosa showed that the number of [gG-and IgM-positivc plasma ceils and the staining intensity for IgG and C3 were increased in earrageenan-indueed colitis, but these changes were inhibited by K-76. Besides, serum mueoprotein concentrations and CHmi levels were lower in the animals treated with carrageenan alone. K-76 exerted multifunctional activity, although its mechanisms of action remain obscure.

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“…An elevated ratio of CD4/CD8 in the lamina propria represents hyperimmune status in the mucosa, and is likely to stimulate B cells to produce antibodies including anti-colon antibodies. Colonic epithelial cells and monocytes in the mucosa express HLA-DR through the stimulation of cytokines (interferon-), and show antigenpresenting property, which leads mucosal inflammation to complexed and more continuous one (3)(4)(5).…”

Section: Inflammatory Bowel Disease and Immunologymentioning

confidence: 99%