Immunoglobulin Determinants on the Surface of Human Lymphocytes

Demonstration of Heavy and Light Chain Antigenic Determinants on the Cell-Bound Receptor for Antigen

“…Knowledge of the chemical build up of surface receptors for antigens is essential for further understanding how an immunogen will trigger an immunocompetent cell of relevant specificity into "immunity" or "paralysis." Present information on this issue has been summarized in the introduction to this article and it strongly suggests that the surface receptor carries antigenic specificities in common with serum immunoglobulins (5)(6)(7)(8)(9)(19)(20)(21)(22). Actual inhibition of immunocompetence of cells by treatment with anti-immunoglobulin serum has been achieved in varying experimental conditions (19)(20)(21)(22) and in two of these (19)(20) there is evidence that this takes place through a steric blockage of the uptake of antigen by the cell-bound antibody.…”

The Role of Cell Membrane Immunoglobulin in Primate B Lymphocyte Differentiation

“…B lymphocytes possess easily detectable cell surface immunoglobulin (slg) which has antigen binding specificity identical to that of the antibody molecules secreted by their progeny (Coombs et al 1969, Raff et al 1970. Comprehension of the role that antigen binding by slg may play in B lymphocyte activation has been complicated by the discovery that most human (van Boxel et al 1972, Rowe et al 1973, Knapp et al 1973, Kubo et al 1974, Fu et al 1974, Finkelman et al 1975) and other mammalian B cells (Melcher et al 1974, Abney & Parkhouse 1974, Martin et al 1976, Martin & Leslie 1977, Cuchens & Leslie, 1977, Finkelman & Scher 1979 bear two slg isotypes, sIgM and sIgD, and that these two isotypes on a given cell or cell clone share the same antigen binding specificity and idiotype (Pernis et al 1974, Fu et al 1975, Salsano et al 1974, Goding & Layton 1976.…”

Cells and mediators which participate in immunoglobulin synthesis by human mononuclear cells. II. The mechanism of null cell participation in immunoglobulin synthesis and secretion by B cells

“…This finding by was quickly confirmed by other investigators who also demonstrated the obligatory requirement for T cells in the culture Waldman et al, 1974;Janossy & Greaves, 1975;Keightley, Cooper & Lawton, 1976;Wu et al, 1976;Saxon, Stevens & Ashman, 1977;Fauci & Ballieux, 1979. It was also shown that the circulating human B lymphocytes could be identified by the presence of smIg in high density (Coombs, Feinstein & Wilson, 1969;Raff, 1970;Pernis, Forni & Amante, 1970;Rabellino et al, 1971;Kincade, Lawton & Cooper, 1971;Froland, Natvig & Berdal, 1971;Grey, Rabellino & Pirofsky, 1971), that PWM induced transformation and differentation ofthe B cells (Wu et al, , 1976Keightley et al, 1976), and that these B cells are lacking in individuals presenting with the Bruton (or X-linked) agammaglobulinaemia syndrome Grey et al, 1971;Cooper & Lawton, 1972;Aiuti, Fontana & Gatti, 1973), thus establishing these cells as the immunoglobulin-synthesizing cells. However, the B cells require the presence of monocytes (Gerrard & Fauci, 1981;Rosenberg & Lipsky, 1981;Shin & Choi, 1981;Theile & Lipsky, 1982) and T cells (Keightley et al, 1976;Saxon et al, 1977) in the cultures for immunoglobulin synthesis/secretion to take place.…”