Immunoglobulins in the mouse uterus before implantation

Bernard, O.; Rachman, Flore; Bennett, Dorothea

doi:10.1530/jrf.0.0630237

Cited by 33 publications

(23 citation statements)

References 12 publications

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“…Our results for the mouse uterus show that IgM does not seem to be involved in local secretory immunity during the oestrous cycle, or in early pregnancy; the limited amount detected at the time of implantation was probably due to passive diffusion because of the major increase in vascular permeability at this stage (Psychoyos, 1960). Such increased permeability is probably responsible for the presence of IgG in the uterine stroma at pro-oestrus and oestrus (present study) and at the time of implantation (Bernard et al, 1981). Very few IgGcontaining plasma cells were visualized in the stroma during the oestrous cycle, and oestradiol is known to increase the blood flow as well as an oedematous tendency favouring the passive diffusion of macromolecules (Spaziani & Szego, 1958).…”

Section: Discussionmentioning

confidence: 49%

“…IgA-plasma cells seem to be present throughout the oestrous cycle in rats . Our previous observations suggest that during the preimplantation period of pregnancy in mice, the IgA produced locally by plasma cells is secreted into the uterine lumen via uterine glands, whereas IgG infiltrates the stroma but does not seem to be secreted locally (Bernard, Rachman & Bennett, 1981). This report presents the results of studies of immunoglobulin distribution in the mouse uterus at the various stages of the oestrous cycle.…”

Section: Introductionmentioning

confidence: 80%

“…Our results provide further information on the steps between lymphocyte migration and presence of IgA in the uterine fluid. They indicate that, in mice, IgA-plasma cells infiltrate the stroma during the oestrous cycle and the early stage of pregnancy (Bernard et al, 1981 ) mostly when oestradiol plasma levels are highest. These results also show that IgA is most probably transferred from the plasma cells into the glands, since it was detected in the cells of the glandular epithelium and in the lumina of about 40% of glands at pro-oestrus.…”

Section: Discussionmentioning

confidence: 99%

“…Only mice in which the vaginal epithelial histology and vaginal smears were clearly characteristic of a particular stage of the cycle (Allen, 1922) were further examined for uterine immunoglobulins. Uterine horns were treated as previously described (Bernard, Ripoche & Bennett, 1977;Bernard et al, 1981); briefly, each horn was ligated at both ends, fixed in 4% paraformaldehyde and embedded in polyethylene glycol 1000; 5 µ sagittal and transverse sections were incubated first with rabbit anti-mouse IgA, IgG or IgM (Litton Bionetics, Kensington, Maryland), then with peroxidase-labelled sheep anti-rabbit immunoglobulin (adsorbed with mouse immunoglobulin) (Institut Pasteur Production, Dr M. Lavergne), and stained with diaminobenzidine and H202. Uterine horns from 3-5 mice were studied for each stage of the cycle.…”

Section: Introductionmentioning

confidence: 99%

“…The specificity of the various antisera and conjugates was checked as previously described (Bernard et al, 1981). Endogenous peroxidase activity was only present in eosinophils.…”

Section: Introductionmentioning

confidence: 99%

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Immunoglobulins in the mouse uterus during the oestrous cycle

Rachman¹,

Casimiri²,

Psychoyos³

et al. 1983

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The distribution of IgA, IgG and IgM was studied by an immunoperoxidase technique on sections of mouse uteri at each stage of the oestrous cycle. Staining for IgG and IgA was highest at pro-oestrus, declined at oestrus and was very low during the other stages. At pro-oestrus IgG was found throughout the stroma, in the uterine lumen, and in 10% of glandular lumina; very few IgG-containing plasma cells were present. At pro-oestrus, IgA was found in the uterine lumen, and in most of the uterine glands, both in the lumen and in the epithelium; little IgA was present in the stroma. IgA-plasma cells were detected at each stage of the cycle and were particularly numerous at pro-oestrus and oestrus. These results suggest that IgA is secreted locally from plasma cells into the uterine gland through the glandular epithelium, but that IgG enters the stroma from the local capillaries. The obvious increase in IgG and IgA secretion at pro-oestrus, when plasma oestradiol levels are highest, supports the hypothesis that, during the oestrous cycle, the humoral immune response is regulated in the uterus by ovarian hormones.

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Section: Discussionmentioning

confidence: 49%

Section: Introductionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…The specificity of the various antisera and conjugates was checked as previously described (Bernard et al, 1981). Endogenous peroxidase activity was only present in eosinophils.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Immunoglobulins in the mouse uterus during the oestrous cycle

Rachman¹,

Casimiri²,

Psychoyos³

et al. 1983

Reproduction

Self Cite

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Postnatal uterine development in the rat: Estrogen and antiestrogen effects on luminal epithelium

et al. 1988

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We examined the effects of the synthetic estrogens, diethylstilbestrol (DES) and ethynylestradiol (EE), and the triphenylethylene antiestrogen, clomiphene citrate (CC), on uterine growth and development in the rat. These compounds, unlike estradiol, do not bind significantly to rat serum alphafetoprotein (AFP). Administration of DES or EE during the period of normal uterine gland genesis (postnatal days 10-14) induced luminal epithelium hypertrophy and increased uterine wet weight. The durations of these responses were dose-related. By day 26, luminal epithelium cell numbers were significantly depressed, compared to controls. Uterine gland development was delayed 6 to 9 days, depending upon estrogen dose, and the numbers of uterine glands ultimately achieved were generally less than in untreated control animals. While a daily dose of 0.1 micrograms CC/rat did not alter uterine development, 10 micrograms CC/rat caused prolonged luminal epithelium hypertrophy and inhibited uterine gland genesis without inducing the large increases in uterine weight or the decreases in luminal epithelium cell number seen after estrogen exposure. The number of stromal cells was significantly increased on day 26 after CC exposure. Together with previous studies, these data demonstrate the greater potency and developmental stage specificity of non-AFP-bound estrogens with respect to altering uterine gland development. In addition, these data suggest that the disruptive influence of antiestrogens on gland genesis may be mediated through an indirect influence on the uterine stroma.

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