Immunoglobulins to Group A Streptococcal Surface Molecules Decrease Adherence to and Invasion of Human Pharyngeal Cells

Fluckiger, Ursula; Jones, Kevin F.; Fischetti, Vincent A.

doi:10.1128/iai.66.3.974-979.1998

Cited by 69 publications

(39 citation statements)

References 30 publications

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“…It is interesting to note that other investigators, employing a di¡erent cell culture model, also found a di¡erential e¡ect on adherence and internalization. For example, it was shown that anti-M6 IgG inhibited streptococcal internalization but not adherence [3]. Thus, it is likely that discrete cellular components might be required to mediate adherence and internalization.…”

Section: Discussionmentioning

confidence: 99%

Effect of lipoteichoic acid on the uptake of Streptococcus pyogenes by HEp-2 cells

Sela

2000

FEMS Microbiology Letters

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Lipoteichoic acid (LTA) is thought to play a role in the interactions between Streptococcus pyogenes and host cells. We have examined the effect of exogenous LTA on the adherence and entry of S. pyogenes JRS4 strain into HEp-2 epithelial cells. LTA markedly inhibited bacterial entry in a concentration-dependent manner, up to 250 Wg ml 31 . In contrast, LTA had only a slight inhibitory effect on adherence. LTA also inhibited the entry but not adherence of Salmonella typhimurium strain into HEp-2 cells. Binding experiments showed a dosedependent binding of LTA to cells up to 10 Wg ml 31 . Confocal laser microscopy imaging and analysis revealed that LTA was internalized by the epithelial cells and colocalized with F-actin. These results might imply that, following binding, exogenous LTA enters HEp-2 cells and exerts a cytotoxic effect that interferes with bacterial internalization. A possible target for LTA activity might be the actin cytoskeleton, which is known to be essential for bacterial uptake. ß

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Section: Discussionmentioning

confidence: 99%

Effect of lipoteichoic acid on the uptake of Streptococcus pyogenes by HEp-2 cells

Sela

2000

FEMS Microbiology Letters

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“…The molecular mechanisms promoting GAS cell adherence and internalization appear to involve the expression of several virulence factors. Production of capsule material interferes simultaneously with both eukaryotic cell adherence and internalization (Darmstadt et al, 2000;Kawabata et al, 1999;Schrager et al, 1996), while bacterial surface proteins such as SDH, FBP54, and M proteins were found to contribute to adherence and internalization in a serotype or even strain-dependent manner (Courtney et al, 1996;Pancholi and Fischetti, 1997;Cue et al, 1998;Fluckiger et al, 1998;Berkower et al, 1999;Dombek et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

The role played by the group A streptococcal negative regulator Nra on bacterial interactions with epithelial cells

Molinari

Rohde

Talay

et al. 2001

Molecular Microbiology

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Group A streptococci (GAS) specifically attach to and internalize into human epithelial host cells. In some GAS isolates, fibronectin‐binding proteins were identified as being responsible for these virulence traits. In the present study, the previously identified global negative regulator Nra was shown to control the binding of soluble fibronectin probably via regulation of protein F2 and/or SfbII expression in the serotype M49 strain 591. According to results from a conventional invasion assay based on the recovery of viable intracellular bacteria, the increased fibronectin binding did not affect bacterial adherence to HEp‐2 epithelial cells, but was associated with a reduction in the internalization rates. However, when examined by confocal and electron microscopy techniques, the nra‐mutant bacteria were shown to exhibit higher adherence and internalization rates than the corresponding wild type. The mutant bacteria escaped from the phagocytic vacuoles much faster, promoting consistent morphological changes which resulted in severe host cell damage. The apoptotic and lytic processes observed in nra‐mutant infected host cells were correlated with an increased expression of the genes encoding superantigen SpeA, the cysteine protease SpeB, and streptolysin S in the nra‐mutant bacteria. Adherence and internalization rates of a nra/speB‐double mutant at wild‐type levels indicated that the altered speB expression in the nra mutant contributed to the observed changes in both processes. The Nra‐dependent effects on bacterial virulence were confined to infections carried out with stationary growth phase bacteria. In conclusion, the obtained results demonstrated that the global GAS regulator Nra modulates virulence genes, which are involved in host cell damage. Thus, by helping to achieve a critical balance of virulence factor expression that avoids the injury of target cells, Nra may facilitate GAS persistence in a safe intracellular niche.

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“…The secretory IgA (sIgA) and IgG antibodies have been found to block S. pyogenes M protein-mediated adhesion and invasion of human pharyngeal cells [7]. We postulated that binding of Mig to IgA, IgG or anti-Mig serum might interfere with the bacterial interaction with the host cells since the Mig protein possesses similar antiphagocytic properties to the M protein.…”

Section: Mig-mediated Binding Properties Are Not Involved In S Dysgamentioning

confidence: 99%

The Mig protein ofStreptococcus dysgalactiaeinhibits bacterial internalization into bovine mammary gland epithelial cells

Song¹,

Perez-Casal²,

Potter³

2004

FEMS Microbiology Letters

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The role of the Mig protein of Streptococcus dysgalactiae in bacterial adhesion and internalization of bovine mammary gland epithelial cells (MAC-T) was investigated with the wild-type and isogenic mig mutant strains. While there was no difference in adhesion between the strains, the wild-type strain exhibited a significantly lower level of invasion than the mutants. The lower level of internalization of the Mig(+) strain is likely due to Mig-mediated interference with uptake of the microorganisms rather than the host protein binding properties of Mig. Avoidance of intimate interactions with the host cells might be an alternative strategy for S. dysgalactiae to survive and persist in the bovine mammary glands.

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Immunoglobulins to Group A Streptococcal Surface Molecules Decrease Adherence to and Invasion of Human Pharyngeal Cells

Cited by 69 publications

References 30 publications

Effect of lipoteichoic acid on the uptake of Streptococcus pyogenes by HEp-2 cells

Effect of lipoteichoic acid on the uptake of Streptococcus pyogenes by HEp-2 cells

The role played by the group A streptococcal negative regulator Nra on bacterial interactions with epithelial cells

The Mig protein ofStreptococcus dysgalactiaeinhibits bacterial internalization into bovine mammary gland epithelial cells

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