Immunogold labelling of beet necrotic yellow vein virus particles inside its fungal vector, Polymyxa betae K

Ryšánek, P.; Stocky, G.; Haeberlé, A. M.; Putz, C.

doi:10.1051/agro:19920808

Cited by 29 publications

(18 citation statements)

References 5 publications

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“…Langenberg and Giunchedi [14] found virus in contact with plasmodia and zoosporangia, whereas Rysanek et al [22] observed some particles in cells containing these developmental stages of P. betae, but also in young plasmodia, zoosporangia and immature zoospores. Rysanek et al [22] observed in some cases virus in about half of the plasmodia and suggested that only a part of the spores would carry virus after differentiation of plasmodia into zoosporangia or cystosori. By diluting soil Tuitert [26] estimated that only 10-15% of the infective population of P. betae in heavily infested soil was viruliferous.…”

Section: Discussionmentioning

confidence: 97%

“…This might be due to the extent in which secondary zoospores of P. betae contain virus. The amount of virus in the fungus probably depends on the amount of virus in the host cell at the time of development of the fungus into secondary zoospores or cystosori [22]. Virus-like particles were observed in thin sections of P. betae zoospores by electron microscopy by Tamada [25].…”

Section: Discussionmentioning

confidence: 99%

“…Virus-like particles were observed in thin sections of P. betae zoospores by electron microscopy by Tamada [25]. Langenberg and Giunchedi [14] found virus in contact with plasmodia and zoosporangia, whereas Rysanek et al [22] observed some particles in cells containing these developmental stages of P. betae, but also in young plasmodia, zoosporangia and immature zoospores. Rysanek et al [22] observed in some cases virus in about half of the plasmodia and suggested that only a part of the spores would carry virus after differentiation of plasmodia into zoosporangia or cystosori.…”

Section: Discussionmentioning

confidence: 99%

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In situ localisation of beet necrotic yellow vein virus (BNYVV) in rootlets of susceptible and resistant beet plants

Scholten¹,

Paul²,

Peters

et al. 1994

Archives of Virology

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Mechanisms of resistance to beet necrotic yellow vein virus (BNYVV) were studied by comparing the multiplication and distribution of BNYVV in root tissue of some beet accessions. Seedlings were infected either by soil containing resting spores of Polymyxa betae with BNYVV, or by a viruliferous zoospore suspension. With both inoculation methods high virus concentrations were obtained in rootlets of the susceptible cultivar 'Regina'. Using infested soil, low virus concentrations were found in the partially resistant cultivar 'Rima' and in the resistant accessions Holly and WB42. When a zoospore suspension was used, similar virus concentrations occurred in 'Rima' and Holly as in 'Regina', while a low virus concentration was found in WB42. By in situ localisation studies, using immunogold-silver labelling, virus was detected in 'Regina' after infection by soil or a zoospore suspension, but it could only be detected in the resistant accessions after infection by a zoospore suspension. In rootlets of 'Regina', 'Rima' and Holly, virus was found in the epidermis, cortex parenchyma, endodermis, and interstitial parenchyma, but in general not inside the vascular tissue. In WB42 the virus, occurring in small aggregates, seemed to be restricted to the epidermis and some cortex parenchyma cells. Comparing both the multiplication and distribution of BNYVV in rootlets of the accessions studied, it is concluded that the virus resistance mechanism in 'Rima' and Holly is different from that in WB42.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

In situ localisation of beet necrotic yellow vein virus (BNYVV) in rootlets of susceptible and resistant beet plants

Scholten¹,

Paul²,

Peters

et al. 1994

Archives of Virology

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show abstract

“…Adams et al (2001) suggested that in the case of viruses transmitted by plasmodiophorid vectors, transmembrane regions present in the coat protein readthrough could play a role in facilitating particle movement across the membrane. Direct evidence for the presence of virus in zoospores and sporangia has been obtained in the case of Beet necrotic yellow vein virus (BNYVV) and BaMMV (Chen et al 1991;Dubois et al 1994;Rysanek et al 1992). In plasmodiophorid-transmitted viruses, this might occur with the aid of the two CP transmembrane regions (Adams et al 2000).…”

Section: Development Cycle Of Plasmodiophoridsmentioning

confidence: 99%

The Fungal Transmitted Viruses

2008

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“…This genus of viruses was first proposed as the Furovirus genus (fungusborne rod-shaped virus group) by Shirako and Brakke in 1984 (Brown, 1989 (Jupin et al, 1991;Shirako and Wilson, 1993;Scott et al, 1994;Kashiwazaki et al, 1995 (Campbell, 1993 relationship has been identified using immunogold labelling-electron microscopy to localize barley mild mosaic bymovirus (BaMMV) and BNYVV particles within the plasmodia and zoospores of P graminis and P betae, respectively (Chen et al, 1991;Rysanek et al, 1992). Only a small proportion (1-2%) of the zoospores appeared to carry virus, but once a zoospore is viruliferous, the number of virions it carries is very large, up to 3 000-7 000 particles (Chen et al, 1991 …”

Section: Introductionmentioning

confidence: 99%