Immunogold localization of arabinogalactan proteins, unesterified and esterified pectins in pollen grains and pollen tubes ofNicotiana tabacum L.

Li, Yi Qin; Faleri, Claudia; Geitmann, Anja; Zhang, Hong Qi; Cresti, Mauro

doi:10.1007/bf01280289

Cited by 107 publications

(80 citation statements)

References 36 publications

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“…Moreover, after the second pollen mitosis, the two resulting sperm cells that are inside the pollen grain or inside the pollen tube are still strongly labelled by these two mAbs. The specific labelling of the generative cell was also reported for oilseed rape (Pennell et al, 1991), for Nicotiana tabacum (Li et al, 1995) and for Brassica campestris male gametes (Southworth & Kwiatkowski, 1996). JIM8 and JIM13 do not label the pollen tube wall, which instead is labelled by MAC207 and LM2 (Pereira et al, 2006).…”

Section: Immunolocalization Of Glycan Epitopesmentioning

confidence: 92%

Arabinogalactan Proteins in Arabidopsis thaliana Pollen Development

Coimbra¹,

Pereira²

2012

Transgenic Plants - Advances and Limitations

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Section: Immunolocalization Of Glycan Epitopesmentioning

confidence: 92%

Arabinogalactan Proteins in Arabidopsis thaliana Pollen Development

Coimbra¹,

Pereira²

2012

Transgenic Plants - Advances and Limitations

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“…In many species, such as tobacco (Li et al, 1995), Arabidopsis (Van Aelst and Van Went, 1992; Rhee and Somerville, 1998), Lilium hybrida (Aouali et al, 2001), Euphorbia peplus (Suárez-Cervera et al, 2002), Zygophyllum fabago (Castells et al, 2003), and Larix decidua (Rafinska et al, 2014), the intine of mature pollen grains is mostly composed of weakly methylesterified HG or a mix of highly and weakly methylesterified HGs, the latter being more abundant. The almost absent labeling of weakly methylesterified HG with John Innes Monoclonal5 at the pollen mother cell and tetrad stages (Rhee and Somerville, 1998) but its strong detection in the intine at the late microspore stage and mature dry Arabidopsis pollen grains (Van Aelst and Van Went, 1992; Rhee and Somerville, 1998) indicate an early action of PMEs during pollen formation and maturation.…”

Section: Discussionmentioning

confidence: 99%

“…New membranes and cell wall materials are rapidly secreted at the tip, providing the building material necessary to sustain the fast pollen tube growth (Bove et al, 2008;Guan et al, 2013). The pollen tube cell wall of many species, including tobacco (Nicotiana tabacum; Li et al, 1995;Ferguson et al, 1998), lily (Lilium longiflorum; Roy et al, 1997), and Arabidopsis (Arabidopsis thaliana; Lennon and Lord, 2000;Dardelle et al, 2010), is characterized by one layer in the tip region and two distinguishable layers back in the shank. Back from the tip, the inner layer is mainly composed of callose, a (1,3)-b-glucan, which is not detectable in the tip region in normal growth conditions (Ferguson et al, 1998;Derksen et al, 2002;Parre and Geitmann, 2005b;Dardelle et al, 2010;Chebli et al, 2012).…”

mentioning

confidence: 99%

PECTIN METHYLESTERASE48 Is Involved in Arabidopsis Pollen Grain Germination

et al. 2014

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Germination of pollen grains is a crucial step in plant reproduction. However, the molecular mechanisms involved remain unclear. We investigated the role of PECTIN METHYLESTERASE48 (PME48), an enzyme implicated in the remodeling of pectins in Arabidopsis (Arabidopsis thaliana) pollen. A combination of functional genomics, gene expression, in vivo and in vitro pollen germination, immunolabeling, and biochemical analyses was used on wild-type and Atpme48 mutant plants. We showed that AtPME48 is specifically expressed in the male gametophyte and is the second most expressed PME in dry and imbibed pollen grains. Pollen grains from homozygous mutant lines displayed a significant delay in imbibition and germination in vitro and in vivo. Moreover, numerous pollen grains showed two tips emerging instead of one in the wild type. Immunolabeling and Fourier transform infrared analyses showed that the degree of methylesterification of the homogalacturonan was higher in pme482/2 pollen grains. In contrast, the PME activity was lower in pme482/2, partly due to a reduction of PME48 activity revealed by zymogram. Interestingly, the wild-type phenotype was restored in pme482/2 with the optimum germination medium supplemented with 2.5 mM calcium chloride, suggesting that in the wild-type pollen, the weakly methylesterified homogalacturonan is a source of Ca 2+ necessary for pollen germination. Although pollen-specific PMEs are traditionally associated with pollen tube elongation, this study provides strong evidence that PME48 impacts the mechanical properties of the intine wall during maturation of the pollen grain, which, in turn, influences pollen grain germination.

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“…Multiple lines of evidence show associations between AGPs and pectins. Pectins frequently colocalize with AGPs in pollen tubes (Li et al, 1995;Jauh and Lord, 1996;Mollet et al, 2002). Treatment of cell wall fractions with pectin-degrading enzymes allows for the increased release of AGPs (Immerzeel et al, 2006;Lamport et al, 2006).…”

Section: The Role Of Sos5 In Pectin Adherencementioning

confidence: 99%

SALT-OVERLY SENSITIVE5 Mediates Arabidopsis Seed Coat Mucilage Adherence and Organization through Pectins

Griffiths¹,

Tsai²,

Xue

et al. 2014

Plant Physiology

135

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Interactions between cell wall polymers are critical for establishing cell wall integrity and cell-cell adhesion. Here, we exploit the Arabidopsis (Arabidopsis thaliana) seed coat mucilage system to examine cell wall polymer interactions. On hydration, seeds release an adherent mucilage layer strongly attached to the seed in addition to a nonadherent layer that can be removed by gentle agitation. Rhamnogalacturonan I (RG I) is the primary component of adherent mucilage, with homogalacturonan, cellulose, and xyloglucan constituting minor components. Adherent mucilage contains rays composed of cellulose and pectin that extend above the center of each epidermal cell. CELLULOSE SYNTHASE5 (CESA5) and the arabinogalactan protein SALT-OVERLY SENSITIVE5 (SOS5) are required for mucilage adherence through unknown mechanisms. SOS5 has been suggested to mediate adherence by influencing cellulose biosynthesis. We, therefore, investigated the relationship between SOS5 and CESA5. cesa5-1 seeds show reduced cellulose, RG I, and ray size in adherent mucilage. In contrast, sos5-2 seeds have wild-type levels of cellulose but completely lack adherent RG I and rays. Thus, relative to each other, cesa5-1 has a greater effect on cellulose, whereas sos5-2 mainly affects pectin. The double mutant cesa5-1 sos5-2 has a much more severe loss of mucilage adherence, suggesting that SOS5 and CESA5 function independently. Doublemutant analyses with mutations in MUCILAGE MODIFIED2 and FLYING SAUCER1 that reduce mucilage release through pectin modification suggest that only SOS5 influences pectin-mediated adherence. Together, these findings suggest that SOS5 mediates adherence through pectins and does so independently of but in concert with cellulose synthesized by CESA5.

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Immunogold localization of arabinogalactan proteins, unesterified and esterified pectins in pollen grains and pollen tubes ofNicotiana tabacum L.

Cited by 107 publications

References 36 publications

Arabinogalactan Proteins in Arabidopsis thaliana Pollen Development

Arabinogalactan Proteins in Arabidopsis thaliana Pollen Development

PECTIN METHYLESTERASE48 Is Involved in Arabidopsis Pollen Grain Germination

SALT-OVERLY SENSITIVE5 Mediates Arabidopsis Seed Coat Mucilage Adherence and Organization through Pectins

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