Immunohistochemical analysis of in vivo UVB-induced secretion of IL-1α and IL-6 in keratinocytes

Narumi, Hiromi; Nakano, Hajime; Matsuzaki, Yasushi; Sawamura, Daisuke; Hanada, K.

doi:10.3892/mmr.2011.478

Cited by 4 publications

(9 citation statements)

References 10 publications

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“…A single exposure of human subjects to 3 MED of SSL (290−400 nm; 1.4-5.6 J/m 2 ) also caused a rapid increase in TNF-α level in blister fluid that started at 4 h, peaked at 15 h and was normalized 48 h after exposure. On the other hand, IL-1α and IL-1β level in blister fluid was significantly increased only at 15 h and remained elevated to 72 h after SSL exposure 14 . Similarly Hiromi et al showed a rapid up-regulation of IL-1α expression induced by UVB (275-305 nm; 200 mJ/cm 2 ) in the skin of the Hirosaki hairless rats.…”

Section: Discussionmentioning

confidence: 99%

“…IL-10 was increased 24 h after exposure, peaked at 36 h and was cleared at 72 h after irradiation 26 . In human subjects exposed to 3 MED of SSL (290-400 nm), IL-10 increase in skin was detected at 15 and 24 h after irradiation 14 . Local exposure of human volunteers to SSL (1 and 3 MED) induced a strong increase in IL-10 mRNA level 24 h after exposure 13 .…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, UVB irradiation (0.554 mJ/cm 2 ) resulted in a significant decrease in IL-12 levels 24 h after exposure and remained reduced for 4 days 25 . In human subjects exposed to 3 MED of SSL (290-400 nm), no significant effect on IL-12 was found in skin tissue at 4-72 h after irradiation 14 . Although UV light has been reported to stimulate INF-γ production 25 , under our condition we found no effect on the plasma cytokine in either UVB or UVA irradiated mice.…”

Section: Discussionmentioning

confidence: 99%

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Differential modulation of inflammatory markers in plasma and skin after single exposures to UVA or UVB radiation in vivo

Vostalová¹,

Svobodová²,

Galandáková³

et al. 2013

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Background. Solar light generates inflammatory responses in exposed skin. These effects are generally attributed to UVB light. However, skin is exposed to a huge quantum of UVA photons as UVA is a predominant part of sunlight and the radiation used in tanning beds. We examined the effects of a single exposure to UVA and UVB wavebands on cytokine levels in skin and plasma, myeloperoxidase (MPO) activity, expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in skin. Methods. Hairless mice were irradiated with either UVA (10 or 20 J/cm 2 ) or UVB (200 or 800 mJ/cm 2 ). The effects were assessed after 4/24 h. Plasma cytokine levels were evaluated using a Bio-Plex cytokine assay. Cytokine, iNOS and COX-2 levels in skin were determined by Western blot. Skin MPO activity was monitored spectrophotometrically. Results. UVB induced up-regulation of interleukin-1β (IL-1β) and interleukin-6 (IL-6) and decrease in interleukin-10 (IL-10) mainly after 4 h. In contrast, UVA caused increase in levels of tumor necrosis factor-alpha (TNF-α) and IL-6 after 4 h and up-regulated IL-10 and interleukin-12 (IL-12) after 24 h. The increase in MPO activity from infiltrated leucocytes was observed only in UVB irradiated animals. iNOS was up-regulated 4 h after UVA and UVB treatment. No significant effect on COX-2 expression was detected. Conclusions. UVA and UVB light affected several inflammatory markers. For individual waveband, changes in plasma parameters did not correlate with those in skin. Thus evaluation of plasma samples cannot simply be replaced by determination in skin specimens and vice versa.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Differential modulation of inflammatory markers in plasma and skin after single exposures to UVA or UVB radiation in vivo

Vostalová¹,

Svobodová²,

Galandáková³

et al. 2013

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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“…Keratinocytes populate the stratified epidermis, the outer layer of the skin, and perform vital functions in defense, regeneration, wound healing, and homeostasis . Keratinocytes secrete a variety of growth factors and cytokines to regulate proliferation, migration, differentiation, and apoptosis in the epidermis …”

Section: Introductionmentioning

confidence: 99%

“…7 Keratinocytes secrete a variety of growth factors and cytokines to regulate proliferation, migration, differentiation, and apoptosis in the epidermis. [8][9][10] Cell migration proceeds through a series of steps precisely controlled by a cellular signaling network and the establishment of extracellular matrix proteins. 11 Keratinocytes treated with epidermal growth factor (EGF) markedly increase migratory activity through activation of the extracellular signal-regulated kinase pathway.…”

Section: Introductionmentioning

confidence: 99%

Leucine‐rich glioma inactivated 3 promotes HaCaT keratinocyte migration

Jeong

Park

Kim

et al. 2013

Wound Repair Regeneration

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Our finding that human skin expresses leucine-rich glioma inactivated 3 (LGI3) raises the question of the function of this cytokine in keratinocytes. We have shown that LGI3 stimulates human HaCaT keratinocyte migration without affecting viability or proliferation. Western blot analysis showed that LGI3 induced focal adhesion kinase activation, Akt phosphorylation, and glycogen synthase kinase 3β (GSK3β) phosphorylation in these cells. Using the scratch wound assay and a modified Boyden chamber, we found that LY294002, a selective phosphatidylinositol 3-kinase inhibitor, and LiCl, a selective GSK3β inhibitor, abolished LGI3-induced cell migration. We tested β-catenin levels after LGI3 treatment because the Akt-GSK3β pathway regulates β-catenin accumulation, and β-catenin promotes cell migration. LGI3 treatment increased β-catenin protein and nuclear localization, whereas LY294002 prevented LGI3-induced focal adhesion kinase and Akt activation as well as β-catenin accumulation. Overall, these data suggest that LGI3 stimulates HaCaT cell migration following β-catenin accumulation through the Akt pathway.

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Inhibitory Effects of Collagen Coated Coffee Bean Intake on Skin Aging

Lee¹,

Ha²,

Shin³

2019

J. People Plants Environ

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To evaluate the protective effect of collagen peptide-coated coffee extract on skin aging, cell viability was measured with a MTT assay using cultured CCD-986sk fibroblasts, and its effect on wrinkles in the skin of hairless mice induced by UVB-irradiation was examined. In addition, its effect on procollagen synthesis and anti-oxidative, and its inhibitory activity against collagenase, elastase, tyrosinase and MMP-1 were analysed. After the 30-minute topical treatment, the animals were exposed to UVB irradiation (60-100 mJ/cm 2) for 4 weeks and its intensity increased during the period. Under the experimental conditions set in this study, the skin thickness of hairless mice significantly decreased (11.8-21.3%) compared to the control group. Based on these results, the prolonged oral intake of a collagen peptide mixture with coffee is expected to significantly increase the synthesis of procollagen in dermal fibroblasts, thereby contributing to the alleviation of wrinkling and lowered elasticity due to structural damage to the dermal layer caused by UV. The oral intake of collagen-coated coffee contributes to increasing collagen biosynthesis in a dose-dependent manner and alleviates the symptoms of thickened keratin caused by UV irradiation. However, it did not inhibit the enzymes involved in skin aging, whitening, wrinkle improvement, and antioxidation. Based on the these results, it can be concluded that the intake of collagen peptide-coated coffee extract can be utilized as an alternative material for the prevention or treatment of diseases associated with photoaging.

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Immunohistochemical analysis of in vivo UVB-induced secretion of IL-1α and IL-6 in keratinocytes

Cited by 4 publications

References 10 publications

Differential modulation of inflammatory markers in plasma and skin after single exposures to UVA or UVB radiation in vivo

Differential modulation of inflammatory markers in plasma and skin after single exposures to UVA or UVB radiation in vivo

Leucine‐rich glioma inactivated 3 promotes HaCaT keratinocyte migration

Inhibitory Effects of Collagen Coated Coffee Bean Intake on Skin Aging

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