Immunohistochemical analysis of microsomal glutathione S-transferase 1 and clusterin expression in lens epithelial cells of patients with pseudoexfoliation syndrome

Abstract

Abstract. Pseudoexfoliation syndrome (PEX) is an age-associated, sight disorder affecting elastic fibers in the eye and visceral organs but its exact etiology remains unknown. The purpose of the current study was to determine the morphology and ultrastructure of lens epithelial cells (LECs), and to use immunohistochemistry to examine localization of microsomal glutathione S-transferase 1 (MGST1) and clusterin. Anterior lens capsules were obtained from 24 patients (13 PEX and 11 controls) who underwent phacoemulsification. Immunohistochemistry was performed, using antibodies against MGST1 and clusterin, to determine their expression. The morphology and ultrastructure of LECs were evaluated by light and transmission electron microscopy, respectively. The PEX LECs were characterized by significantly lower MGST1 (P= 0.0001) and clusterin expression (P= 0.0005) compared with the control group patients. PEX LECs were also observed to have significantly increased thickness compared with the control group patients (P= 0.0002). The current findings suggest that low MGST1 and clusterin expression levels may be an early clinical indicator of PEX, and that oxidative stress may serve an important role, but that the specific etiology of this disease has yet to be revealed.

Recent advances in risk factors associated with ocular exfoliation syndrome

“…Although clusterin is over-expressed in various stress and pathological conditions, it is downregulated in the anterior chamber of the exfoliative eyes. This suggests the possible contribution of common CLU variants in exfoliation development (Calero et al 2000;Zenkel et al 2006;Magalhães & Saraiva 2011;Padhy et al 2014;Fan et al 2015;Stafiej et al 2017). A CLU SNP (rs3087554) was observed in an Australian population that was identified to be nominally associated with XFS.…”

“…; Stafiej et al. ). A CLU SNP (rs3087554) was observed in an Australian population that was identified to be nominally associated with XFS.…”

Pseudoexfoliation glaucoma: Always actual problem in ophthalmology

“…Nađeni su: heparin i hondroitin sulfat proteoglikani, laminin, entaktin, nidogen, fibronektin i amiloid P protein; kolagen tipa IV je ograničen na mikrofibrilarni sloj između površine kapsule i tipičnog PEX materijala (24). Dodatno, prisustvo epitopa elastina ukazuje da je PEX materijal multikomponentna ekspresija poremećaja u sintezi ekstraćelijskog matriksa, uključujući inkorporaciju osnovnih nekolagenih komponenti bazalne membrane (25,26). Ekstenzivna obeleženost materijala na hondroitin sulfat pokazuje preveliku produkciju i abnormalni metabolizam glikozaminoglikana kao osnovne promene u ovom poremećaju (27).…”

Pseudoexfoliation and Cataract Syndrome Associated with Genetic and Epidemiological Factors in a Mayan Cohort of Guatemala

“…Pseudoexfoliation syndrome (PXF) was first described in 1917 by John G. Lindberg [ 1 , 2 , 3 ]. The term pseudoexfoliation is used to distinguish from the similarly appearing, but uncommon and clinically different exfoliation syndrome of anterior capsular damage occurring in extreme infrared radiation exposure in glass blowers [ 1 , 2 , 3 ]. PXF is a systemic disease in which tissues in the body accumulate white/gray material consistent with extrafibrillar material [ 1 , 2 , 3 ].…”

“…The term pseudoexfoliation is used to distinguish from the similarly appearing, but uncommon and clinically different exfoliation syndrome of anterior capsular damage occurring in extreme infrared radiation exposure in glass blowers [ 1 , 2 , 3 ]. PXF is a systemic disease in which tissues in the body accumulate white/gray material consistent with extrafibrillar material [ 1 , 2 , 3 ]. In the eye, PXF material accumulates chronically and progressively in both extraocular and intraocular tissue due to excess production and/or decreased breakdown of elastic microfibrillar components [ 4 ].…”