Immunohistochemical and electron‐microscopic identification of neuroendocrine cells in the stomach of uremic rats

Abstract

Many disturbances in electrolyte and hormonal balance in the body induced by functional impairment of renal parenchyma may affect the activity of amine precursor uptake and decarboxylation (APUD) cells, which constitute a very important link in the regulation of homeostasis. The aim of the present study was the morphological, immunohistochemical and ultrastructural estimation of enteroendocrine cells in the stomach of uremic rats. Fragments of gastric pylorus were collected 1, 2 and 4 weeks after nephrectomy. Paraffin embedded sections were stained with H + E and by silver impregnation. For identification of neuroendocrine cells, immunohistochemical reactions were performed using specific antibodies against somatostatin, synaptophysin, neuron-specific enolase and anti-calcitonin gene related peptide. The analysis showed an increased number of APUD cells in the stomach of uremic rats compared to control rats, which may be a morphological expression of their hyperfunction in the functional impairment of renal parenchyma. These results suggest that chronic renal failure can modulate the secretory processes of APUD cells.

Proliferative and Nonproliferative Lesions of the Rat and Mouse Respiratory Tract

“…In neuroendocrine cells of the larynx and trachea, serotonin could not be demonstrated, whereas in the lung, serotonin-containing cells were seen. (Adriaensen et al 2001;Boorman, Morgan, and Uraih 1990;Dungworth, Ernst, et al 1992;Dungworth et al 2001;Elizegi et al 2001;Gopinath, Prentice, and Lewis 1987;Haworth et al 2007;Karube et al 1989;Kasacka and Sawicki 2004;Kerns et al 1983;Larson et al 2004;Lauweryns and Van Ranst 1988;Lauweryns et al 1987;D. Lewis 1991;Luts et al 1991;Maronpot et al 1986;McBride et al 1990;Montuenga et al 1992;Pack, Al-Ugaily, and Morris 1981;Rehm and Kelloff 1991;Shimosegawa and Said 1991;Takahashi, Iwasaki, and Ide 1985;Van Lommel 2001;Van Lommel et al 1999) Hyperplasia with Cellular Atypia (Dysplasia): Larynx, Trachea, Bronchi, Bronchioles Pathogenesis/cell of origin: Proliferation of respiratory epithelium, or submucosal glands.…”

“…Diagnosis of a malignant tumor is based on one or more features including overall size, loss of polarity of epithelium, a high degree of atypia, increased numbers of mitoses, or invasive behavior. (Haworth et al 2007;Kasacka and Sawicki 2004;Levasseur et al 2004;Reznik, Stinson, and Ward 1980;Reznik-Schüller 1983a, 1983bRouquier and Giorgi 2007;Schüller, Gregg, and Reznik 1990;Shimosegawa and Said 1991;Trinh and Storm 2004) Hyperplasia with Atypia ( Figure 40): Nasal Cavity, Nasopharynx, Paranasal Sinus Pathogenesis/cell of origin: Hyperplastic squamous, respiratory, olfactory, or glandular epithelium. Diagnostic features:…”

“…Although neuroendocrine cell hyperplasias in mice are described above, there are no reports of neuroendocrine tumors in mice. (Elizegi et al 2001;Haworth et al 2007;Ito et al 1989;Kasacka and Sawicki 2004;Larson et al 2004;Lauweryns and Van Ranst 1988;McBride et al 1990;Montuenga et al 1992 Metastases from squamous cell carcinoma with its primary at another organ site are mostly multifocal and perivascular.…”

Gastrointestinal neuroendocrine cells in various types of hypertension – a review

“…Their number is estimated as 3x10 9 [8]. Enteroendocrine cells have a variety of shapes [4,5,17], in H+E staining -a dark nucleus is surrounded by a bright, weakly stained cytoplasm. In terms of morphology, they can be divided into two types: the open type, when the top surface of the cell contacts the lumen of the digestive tract (Fig.…”