Immunohistochemical collagen analysis of the most superficial layer in adult articular cartilage

Teshima, Ryota; Ono, Masanori; Yamashita, Y; Hirakawa, Hiromasa; Nawata, Kouji; Morio, Yasuo

doi:10.1007/s00776-004-0769-4

Cited by 23 publications

(26 citation statements)

References 14 publications

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“…In nonoperated control sections (Fig. 2a, c), type I collagen was restricted to the uppermost surface lamina, as reported previously [29]. Type III collagen, which is typically seen pericellularly in normal cartilage [30], also exhibited increased matrix staining after meniscectomy (Fig.…”

Section: Resultssupporting

confidence: 83%

“…Type III collagen is present pericellularly in small amounts in normal articular cartilage [16,30], and type I collagen is is evident in the most superficial layer [29]. Contrary to early reports [39], evidence now suggests that both types I and III collagens are significantly increased in OA cartilage, both at the expression and protein levels [40,41].…”

Section: Discussionmentioning

confidence: 99%

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Regional assessment of articular cartilage gene expression and small proteoglycan metabolism in an animal model of osteoarthritis

Young

Smith

et al. 2005

Arthritis Res Ther

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Osteoarthritis (OA), the commonest form of arthritis and a major cause of morbidity, is characterized by progressive degeneration of the articular cartilage. Along with increased production and activation of degradative enzymes, altered synthesis of cartilage matrix molecules and growth factors by resident chondrocytes is believed to play a central role in this pathological process. We used an ovine meniscectomy model of OA to evaluate changes in chondrocyte expression of types I, II and III collagen; aggrecan; the small leucine-rich proteoglycans (SLRPs) biglycan, decorin, lumican and fibromodulin; transforming growth factor-β; and connective tissue growth factor. Changes were evaluated separately in the medial and lateral tibial plateaux, and were confirmed for selected molecules using immunohistochemistry and Western blotting. Significant changes in mRNA levels were confined to the lateral compartment, where active cartilage degeneration was observed. In this region there was significant upregulation in expession of types I, II and III collagen, aggrecan, biglycan and lumican, concomitant with downregulation of decorin and connective tissue growth factor. The increases in type I and III collagen mRNA were accompanied by increased immunostaining for these proteins in cartilage. The upregulated lumican expression in degenerative cartilage was associated with increased lumican core protein deficient in keratan sulphate side-chains. Furthermore, there was evidence of significant fragmentation of SLRPs in both normal and arthritic tissue, with specific catabolites of biglycan and fibromodulin identified only in the cartilage from meniscectomized joints. This study highlights the focal nature of the degenerative changes that occur in OA cartilage and suggests that altered synthesis and proteolysis of SLRPs may play an important role in cartilage destruction in arthritis.

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Section: Resultssupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Regional assessment of articular cartilage gene expression and small proteoglycan metabolism in an animal model of osteoarthritis

Young

Smith

et al. 2005

Arthritis Res Ther

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“…Of particular note is the dramatic influence of HA on the elastic modulus of the hydrogel surface, given that it has been reported that cultured bovine articular cartilage has been shown to release 50% of all synthesized HA into the culture medium, whereas only 5% of all proteoglycans were discarded (43). A survey of the literature suggests that the precise connective tissue composition of articular cartilage (44, 45) is still under debate, as is its role in moving load‐bearing joints (46). In fact previously unrecognized molecular components of articular cartilage, such as Del1 (47), continue to be reported.…”

Section: Discussionmentioning

confidence: 99%

Poly(vinyl alcohol) Hydrogel as a Biocompatible Viscoelastic Mimetic for Articular Cartilage

Grant

Twigg

Egan

et al. 2006

Biotechnology Progress

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“…Furthermore, the long-term survival of engineered implants in the hostile environment of an osteoarthritic joint may depend on the capacity to establish a population of progenitor cells within the surface zone that can drive a repair process when damage has accrued. It is important to note that at least one study [46] has shown a lack of type II collagen in this zone. Instead the parallel bundles of collagen fibrils were found to be composed of collagen types I and III.…”

Section: Development and Regeneration Of The Surface Of Articulating mentioning

confidence: 95%

Stem Cells and Cartilage Development: Complexities of a Simple Tissue

2010

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Cartilage is considered to be a simple tissue that should be easy to engineer because it is avascular and contains just one cell type, the chondrocyte. Despite this apparent simplicity, regenerating cartilage in a form that can function effectively after implantation in the joint has proven difficult. This may be because we have not fully appreciated the importance of different structural regions of articular cartilage or of understanding the origins of chondrocytes and how this cell population is maintained in the normal tissue. This review considers what is known about different regions of cartilage and the types of stem cells in articulating joints and emphasizes the potential importance of regeneration of the lamina splendens at the joint surface and calcified cartilage at the junction with bone for long-term survival of regenerated tissue in vivo. Stem Cells 2010;28:1992–1996

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Immunohistochemical collagen analysis of the most superficial layer in adult articular cartilage

Cited by 23 publications

References 14 publications

Regional assessment of articular cartilage gene expression and small proteoglycan metabolism in an animal model of osteoarthritis

Regional assessment of articular cartilage gene expression and small proteoglycan metabolism in an animal model of osteoarthritis

Poly(vinyl alcohol) Hydrogel as a Biocompatible Viscoelastic Mimetic for Articular Cartilage

Stem Cells and Cartilage Development: Complexities of a Simple Tissue

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