Immunohistochemical Demonstration of Cytokeratin is Useful for Detecting Micrometastatic Foci from Gallbladder Carcinoma in Regional Lymph Nodes

Tajima, Yoshitsugu; Tomioka, Tsutomu; Ikematsu, Yoshito; Ichinose, Kunihiro; Inoue, Keiji; Kanematsu, Takashi

doi:10.1093/jjco/29.9.425

Cited by 17 publications

(12 citation statements)

References 14 publications

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“…KL-1 reacts with cytokeratins (CK), components of intracytoplasmic network of intermediate filaments (Ifs) [4,8,35] , specifically reacts with epithelial tumors. Anti-CK mABs using immunocytochemistry or CK RT-PCR have been widely used for the detection of micrometastases in lymph nodes, venous blood and bone marrow [1,4,12,28,[36][37][38][39][40][41][42] . It is satisfactory using KL-1 monoclonal antibody to detect micrometastasis in the lungs and livers of the mice in this study.…”

Section: Discussionmentioning

confidence: 99%

Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases

Cui

Krueger²,

Henne‐Bruns³

et al. 2001

WJG

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AIM To establish a relevant animal model of human gastrointestinal cancer, which can be used for repetitive investigations, so as to improve our understanding and management of carcinogenesis and cancer metastasis. METHODS Intact tissues of human colorectal and pancreatic cancers were transplanted in nude mice. The biological characteristics of the original and the corresponding transplanted tumors were investigated by HE staining, PAS staining and immunostaining. The metastases in the livers and lungs of nude mice were i n v e s t i g a t e d b y i m m u n o s t a i n i n g w i t h biotinylated mab KL-1 and by RT-PCR using CK20 specific primers. RESULTS There were totally 9 of 16 surgical s p e c i m e n s g r o w i n g i n n u d e m i c e subcutaneously and/or orthotopically (4 of 6 colorectal and 5 of 10 pancreatic cancer). Tumor cell content of the specimens and freezing of tissue specimens are important factors influencing the growth of transplanted tu m o r . I n t h e g r o u p o f f r e s h t u m o r tissues with greater than 50% tumor cell content, the success rate of the transplantation was 100% (3 cases of pancreatic cancer and 3 cases of colorectal cancer). The orthotopically transplanted tumors resemble the original tumor morphologically and biologically, including T A A e x p r e s s i o n s u c h a s C E A b y immunohistochemistry, and CEA level in the serum of mice. Ki-67 labeling index and the expression of TAA especially K-ras, 17-1A and RA-96, are associated with the potential of tumor growth in nude mice. Micrometastases in the lungs and livers of tumor bearing mice can be detected by immunostaining with biotinylated mab KL-1 and CK20-specific RT-PCR. CONCLUSION An orthotopic transplantation model for human colon and pancreatic cancer in nude mice has been set up. We have also established sensitive detection methods with CK-immunohistochemistry and CK20-RT-PCR to study xenotransplanted human cancer and its metastatic cancer cells in the liver and lung of nude mice. This study may be helpful in understanding the mechanism of cancer metastasis and in developing new d i a g n o s t i c m e t h o d s a n d t h e r a p e u t i c s t r a t e g i e s f o r m e t a s t a s e s i n c l u d i n g micrometastases.

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Section: Discussionmentioning

confidence: 99%

Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases

Cui

Krueger²,

Henne‐Bruns³

et al. 2001

WJG

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“…Other series using either cytokeratin subtype 7 or 8/18 have also shown that cytokeratin immunostains are highly sensitive in detecting BD and GB cancers [17][18][19]. Both EMA and CEA were less appealing as immunostains, because of weaker staining in histologically positive BD and GB cancers (Figs.…”

Section: Discussionmentioning

confidence: 96%

“…The incidence of micrometastases in patients with breast, melanoma, or GI cancers ranges widely, between 10% and 50%, and the clinical significance of this finding is highly variable between studies [8, 10, 14 -16]. Unfortunately, only a few studies have evaluated the incidence and clinical significance of lymph node micrometastases in patients with biliary tract carcinoma [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Cytokeratin Is a Superior Marker for Detection of Micrometastatic Biliary Tract Carcinoma

Natarajan

Gilchrist

et al. 2005

Journal of Surgical Research

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“…Tiny aggregates of cancer cells can be detected by means of cytokeratin immunohistochemistry in histologically negative lymph nodes (Yokoyama et al 1999;Natarajan et al 2005;Sasaki et al 2006). In one study, 7 out of 255 HE-negative lymph nodes (2.7 %) were found to be positive for micrometastases by the use of cytokeratin immunostaining (Tajima et al 1999). …”

Section: Lymph Node Metastasesmentioning

confidence: 99%

Adenocarcinoma of the Gallbladder (Classical Gallbladder Cancer)

Zimmermann

2016

Tumors and Tumor-Like Lesions of the Hepatobiliary Tract

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Immunohistochemical Demonstration of Cytokeratin is Useful for Detecting Micrometastatic Foci from Gallbladder Carcinoma in Regional Lymph Nodes

Abstract: Cytokeratin staining of dissected lymph nodes is a useful new diagnostic tool for detecting micrometastatic foci in regional lymph nodes of patients with gallbladder carcinoma.

Cited by 17 publications

References 14 publications

Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases

Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases

Cytokeratin Is a Superior Marker for Detection of Micrometastatic Biliary Tract Carcinoma

Adenocarcinoma of the Gallbladder (Classical Gallbladder Cancer)

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