Immunohistochemical demonstration of neuron-specific enolase and microtubule-associated protein 2 in reactive astrocytes after injury in the adult forebrain

Lin, R.C.S.; Matesic, Diane F.

doi:10.1016/0306-4522(94)90199-6

Cited by 44 publications

(15 citation statements)

References 25 publications

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“…Non-neuronal cells, such as the oligodendrocytic precur sor cell (O-2A) and the mast cell, contain high levels of NAA (Urenjak et aI., 1992(Urenjak et aI., , 1993Burlina et aI., 1997). We found no mast cells in or around the infarct, but the fact that reactive astrocytes have the ability to express the fetal traits of progenitor cells, i.e., NSE, GABA, and MAP2 proteins (Lin et aI., 1993;Lin and Matesic, 1994), suggests that they may also contain NAA. The GFAP immunohistochemical staining revealed a massive glio sis from day 2 to 3 along the border of the infarct but not in the infarct core.…”

Section: Tissue Samplingmentioning

confidence: 68%

Correlation between N-Acetylaspartate Levels and Histopathologic Changes in Cortical Infarcts of Mice after Middle Cerebral Artery Occlusion

Sager

Hansen

Laursen

2000

J Cereb Blood Flow Metab

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Summary:The aim of the present study was to evaluate the use of the endogenous neuronal compound N-acetylaspartate (NAA) as a marker of neuronal damage after focal cerebral ischemia in mice. After occlusion of the middle cerebral artery (MCAO) the ischemic cortex was sampled, guided by 2,3,5-triphenyltetrazolium chloride (TTC) staining, and the NAA concentration was measured by high-pressure liquid chroma tography (HPLC). Conventional histology and immunohisto logical methods using antibodies against neuron-specific eno lase (NSE), neurofilaments (NF), synaptophysin, glial fibrillary acidic protein (GFAP), and carbodiamide-linked NAA and N-acetylaspartylglutamate (NAAG). The level of NAA rapidly declined to 50% and 20% of control levels in infarcted tissue after 6 hours and 24 hours, respectively. No further decrease was observed during the observation period of 1 week. Within the first 6 hours the number of normal-appearing neurons in the infarcted cortical tissue decreased to 70% of control, of which the majority were eosinophilic. After 24 hours almost no norIn contrast to other markers of degenerative brain damage, i.e., lactate dehydrogenase, superoxide dismu tase (Lampl et aI., 1990;Strand and Marklund, 1992), and neuron-specific enolase (NSE) (Schaarschmidt et a!., 1994; Steinberg et aI. 1984), the level of N-acetylaspar tate (NAA) can be readily measured noninvasively in situ with a high spatial and temporal resolution by proton magnetic resonance spectroscopy eH-MRS). Thus in Address correspondence and reprint requests to Dr. Thomas N. Sager, Department of Neurodegenerative Disorders, H. Lundbeck AIS, Ottiliavej 9, 2500 Valby, Denmark.Abbreviations used : ABC, avidin-biotin complex; AEC, 3-amino-9-ethylcarbazole; GFAP, glial fibrillary acidic protein; HE, hematoxylin eosin; IH-MRS, proton magnetic resonance spectroscopy; HPLC, high pressure liquid chromatography; HRP, horseradish peroxidase; MCAO, middle cerebral artery occlusion; NAA, N-acetylaspartate; NAAG, N-acetylaspartylglutamate; NF, neurofilaments; NSE, neuron-specific enolase; PAP, peroxidase-anti peroxidase; PBS, phosphate-buffered sa line; TTC, 2,3,5-triphenyltetrazolium chloride. The fact NAA is trapped in cell debris and NAA immunore activity is observed in the peri-infarct areas restricts its use as a marker of neuronal density.

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Section: Tissue Samplingmentioning

confidence: 68%

Correlation between N-Acetylaspartate Levels and Histopathologic Changes in Cortical Infarcts of Mice after Middle Cerebral Artery Occlusion

Sager

Hansen

Laursen

2000

J Cereb Blood Flow Metab

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“…While there was a small proportion of Map2ab + cells with mature neuronal morphology that represented differentiated cells, most of the Map2ab + cells had an immature morphology. It has previously been shown that subsequent to CNS injury, reactive astrocytes can express the neuronal antigens Tau, Map2, neuron-specific enolase, and GABA, in addition to GFAP and nestin (Lin and Matesic, 1994;Frisen et al, 1995;Schinstine and Iacovitti, 1996;Sahin Kaya et al, 1999). It has been hypothesized that reactive astrocytes represent either astrocytes that revert to bipotential progenitors (cells that show both neuronal and glial phenotypes) or that reactive astrocytes are recruited from neural stem cells (Lin et al, 1995;Schinstine and Iacovitti, 1996).…”

Section: Discussionmentioning

confidence: 99%

In vitro growth and differentiation of canine olfactory bulb-derived neural progenitor cells under variable culture conditions

Walton

Wolfe

2008

Journal of Neuroscience Methods

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The dog serves as a large animal model for multiple neurologic diseases that may potentially benefit from neural progenitor cell (NPC) transplantation. In the adult brain, multipotent NPCs reside in the subventricular zone and its rostral and caudal extensions into the olfactory bulb and hippocampus. The olfactory bulb represents a surgically accessible site for obtaining cells for autologous NPC transplantation. To model conditions that would occur for ex vivo gene therapy in the postnatal brain, NPCs were isolated from the canine olfactory bulb, expanded ex vivo under different culture conditions, and compared quantitatively for growth and immunophenotype. Under standard growth conditions, canine olfactory bulb-derived NPCs (OB-cNPCs) could be expanded nearly 500-fold in the time evaluated. Canine OB-cNPCs grown on poly-D-lysine (PDL) or on PDL-fibronectin had similar growth rates, whereas supplementation with leukemia inhibitory factor (LIF) resulted in significantly slower growth. However, when OB-cNPC cultures were grown on PDL-fibronectin or PDL supplemented with LIF, a greater proportion of cells with neuronal markers were generated upon differentiation.

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“…However, despite the name, NSE is also expressed in some glial cells 22 . We therefore confirmed the phenotype of MGE-derived, LacZ-expressing cells by immunocytochemistry and electron microscopy (EM).…”

Section: Dispersed Lacz-positive Mge Cells Are Neuronsmentioning

confidence: 99%

Young neurons from medial ganglionic eminence disperse in adult and embryonic brain

et al. 1999

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In this study, we identified neuronal precursors that can disperse through adult mammalian brain tissue. Transplanted neuronal precursors from embryonic medial ganglionic eminence (MGE), but not from lateral ganglionic eminence (LGE) or neocortex, dispersed and differentiated into neurons in multiple adult brain regions. In contrast, only LGE cells were able to migrate efficiently from the adult subventricular zone to the olfactory bulb. In embryonic brain slices, MGE cells migrated extensively toward cortex. Our results demonstrate that cells in different germinal regions have unique migratory potentials, and that adult mammalian brain can support widespread dispersion of specific populations of neuronal precursors. These findings could be useful in repair of diffuse brain damage.

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Immunohistochemical demonstration of neuron-specific enolase and microtubule-associated protein 2 in reactive astrocytes after injury in the adult forebrain

Cited by 44 publications

References 25 publications

Correlation between N-Acetylaspartate Levels and Histopathologic Changes in Cortical Infarcts of Mice after Middle Cerebral Artery Occlusion

Correlation between N-Acetylaspartate Levels and Histopathologic Changes in Cortical Infarcts of Mice after Middle Cerebral Artery Occlusion

In vitro growth and differentiation of canine olfactory bulb-derived neural progenitor cells under variable culture conditions

Young neurons from medial ganglionic eminence disperse in adult and embryonic brain

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