Immunohistochemical Demonstration of Sensory Nerve Fibers and Endings in Lumbar Intervertebral Discs of the Rat

McCarthy, Peter W.; Carruthers, B.; Martin, David P.; Petts, P.

doi:10.1097/00007632-199106000-00010

Cited by 79 publications

(62 citation statements)

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“…CGRP is found in unmyelinated, slow conducting sensory nerves, which are believed to be involved in nociceptive transmission or neuromodulation (18,28). Although CGRP immunoreactivity has been identified in rat disc (1,25,26,32), in an earlier study in human disc only one of seven…”

Section: Discussionmentioning

confidence: 99%

Neuropeptides in the human intervertebral disc

Ashton¹,

Roberts²,

Jaffray³

et al. 1994

Journal Orthopaedic Research

177

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The innervation of the human intervertebral disc was investigated by immunochemical methods. Immunoreactivity to the general nerve marker protein gene product (PGP 9.5) was found in the outer annulus fibrosus of 11 of 12 discs removed during anterior arthrodesis for back pain. PGP 9.5-immunoreactive fibres ran between and across the collagenous lamellae, both in association with blood vessels and distant from them, and extended at least 3 mm into the disc. No innervation was observed in the nucleus pulposus. Fine fibres (< 1 micron in diameter) immunoreactive to calcitonin gene-related peptide and substance P (neuropeptides located in sensory and possibly nociceptive nerves) were identified in eight and four of the annuli fibrosi, respectively. Nerve fibres immunoreactive to vasoactive intestinal peptide and to the c-flanking peptide of neuropeptide Y were found in the majority of specimens of annulus fibrosus that were examined.

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Section: Discussionmentioning

confidence: 99%

Neuropeptides in the human intervertebral disc

Ashton¹,

Roberts²,

Jaffray³

et al. 1994

Journal Orthopaedic Research

177

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“…In several human and animal studies, sensory nerve fibres in degenerated discs were shown to express painful neuropeptides and growth factors, such as substance P (Ashton et al, 1994;Coppes et al, 1997) and calcitonin gene-related peptide (McCarthy et al, 1991;Roberts et al, 1995) as well as nerve growth factors (Miyagi et al, 2011). There is firm evidence that CS is present in animal models of back injury models.…”

Section: Low Back Painmentioning

confidence: 99%

Assessment and manifestation of central sensitisation across different chronic pain conditions

Arendt-Nielsen

Morlion

Perrot

et al. 2017

European Journal of Pain

517

494

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Different neuroplastic processes can occur along the nociceptive pathways and may be important in the transition from acute to chronic pain and for diagnosis and development of optimal management strategies. The neuroplastic processes may result in gain (sensitisation) or loss (desensitisation) of function in relation to the incoming nociceptive signals. Such processes play important roles in chronic pain, and although the clinical manifestations differ across condition processes, they share some common mechanistic features. The fundamental understanding and quantitative assessment of particularly some of the central sensitisation mechanisms can be translated from preclinical studies into the clinic. The clinical perspectives are implementation of such novel information into diagnostics, mechanistic phenotyping, prevention, personalised treatment, and drug development. The aims of this paper are to introduce and discuss (1) some common fundamental central pain mechanisms, (2) how they may translate into the clinical signs and symptoms across different chronic pain conditions, (3) how to evaluate gain and loss of function using quantitative pain assessment tools, and (4) the implications for optimising prevention and management of pain. The chronic pain conditions selected for the paper are neuropathic pain in general, musculoskeletal pain (chronic low back pain and osteoarthritic pain in particular), and visceral pain (irritable bowel syndrome in particular). The translational mechanisms addressed are local and widespread sensitisation, central summation, and descending pain modulation. Significance Central sensitisation is an important manifestation involved in many different chronic pain conditions. Central sensitisation can be different to assess and evaluate as the manifestations vary from pain condition to pain condition. Understanding central sensitisation may promote better profiling and diagnosis of pain patients and development of new regimes for mechanism based therapy. Some of the mechanisms underlying central sensitisation can be translated from animals to humans providing new options in development of therapies and profiling drugs under development.

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“…England) solution did not reveal any immunoreactivity which could be mistaken for fibers or nerve endings in the discal tissue. As previously reported (McCarthy et al 1991(McCarthy et al , 1992, this technique allowed for a study of' the nucleus pulposus, end-plate, annulus fibrosi, as well as the adjacent ligamentous structures occasionally including periosteum, in 40 jm steps. Estimation of the presence and location of SP-LI structures was an important consideration, as sectioned material was to be used.…”

Section: Methodsmentioning

confidence: 87%

“…However, as was shown previously, there are myelinated fibers in the tissue adjacent to the disc which do not enter the annulus in the same areas as the SP-LI fibers described here (McCarthy et al 1992). Instead, it would appear that myelinated fibers are related to the presence of more complex endings in the adjacent ligaments (Roofe 1940, Malinsky 1959, Yoshizawa et al 1980, Guilliot et al 1988, McCarthy et al 1991, McCarthy et al 1992. Therefore, one may assume that the SP-LI fibers in the rat lumbar intervertebral disc are C-fiberhmmyelinated afferents.…”

Section: Discussionmentioning

confidence: 99%

Sparse substance P-like immunoreactivity in intervertebral discs: Nerve fibers and endings in the rat

McCarthy

1993

Acta Orthopaedica Scandinavica

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Immunohistochemical Demonstration of Sensory Nerve Fibers and Endings in Lumbar Intervertebral Discs of the Rat

Cited by 79 publications

References 0 publications

Neuropeptides in the human intervertebral disc

Neuropeptides in the human intervertebral disc

Assessment and manifestation of central sensitisation across different chronic pain conditions

Sparse substance P-like immunoreactivity in intervertebral discs: Nerve fibers and endings in the rat

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