Immunohistochemical detection of estrogen receptor β in alveolar bone cells of estradiol‐treated female rats: possible direct action of estrogen on osteoclast life span

Cruzoé-Souza, Mady; Sasso‐Cerri, Estela; Cerri, Paulo Sérgio

doi:10.1111/j.1469-7580.2009.01158.x

Cited by 32 publications

(36 citation statements)

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“…Moreover, germ cells strongly ERβ-immunostained were within vacuoles next to Sertoli cells, suggesting phagocytosis, a common event observed during apoptosis [35]. In a recent study made in our laboratory, a similar parallelism between cytoplasmic ERβ overexpression and apoptosis has also been demonstrated in alveolar bone osteoclasts [49]. According to Nilsen et al [50], ERβ acts as a mediator of apoptosis in cultured neurons.…”

Section: Discussionmentioning

confidence: 93%

Enhanced ERbeta immunoexpression and apoptosis in the germ cells of cimetidine-treated rats

Sasso-Cerri

2009

Reprod Biol Endocrinol

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BackgroundCimetidine, refereed as antiandrogenic drug, causes hormonal changes in male patients such as increased testosterone and FSH levels. In the rat testis, structural alterations in the seminiferous tubules have been related to germ cell loss and Sertoli cell death by apoptosis. Regarding the important role of Sertoli cells in the conversion of testosterone into estrogen, via aromatase, the immunoexpression of estrogen receptors-beta (ERbeta) was evaluated in the germ cells of untreated and treated rats with cimetidine. A relationship between ERbeta immunoreactivity and apoptosis was also investigated in the germ cells of damaged tubules.MethodsImmunohistochemistry for detection of ERbeta and TUNEL method were performed in testicular sections of adult male rats treated with 50 mg/Kg of cimetidine (CmG) or saline solution (CG) for 52 days.ResultsIn CG, a cytoplasmic immunoexpression for ERbeta was observed in spermatogonia, primary spermatocytes and spermatids. An evident ERbeta immunoreactivity was always observed in the flagellum and residual bodies of late spermatids. In CmG, the cytoplasm or cytoplasm and nuclei of germ cells of the damaged tubules by cimetidine showed enhanced ERbeta immunostaining. TUNEL-labeling was usually observed in the same germ cell types exhibiting enhanced ERbeta immunoreactivity.ConclusionThe presence of ERbeta immunolabeling in the flagellum and residual bodies of spermatids reinforces the role of estrogen in spermiogenesis. The overexpression of ERbeta in the germ cells of CmG could be related to a possible interference of cimetidine on tubular androgenization and/or on the intratubular aromatase due to Sertoli cell damage. The parallelism between ERbeta overexpression and apoptosis indicates a participation of ERbeta on germ cell death.

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Section: Discussionmentioning

confidence: 93%

Enhanced ERbeta immunoexpression and apoptosis in the germ cells of cimetidine-treated rats

Sasso-Cerri

2009

Reprod Biol Endocrinol

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“…ERα and ERβ have been detected by immunohistochemistry in osteoblasts [28–30], osteocytes [28, 30], and osteoclasts [28–30]. ERα and ERβ are also expressed in immune cells, such as T cells and monocytes [31–33], which are important in bone regulation.…”

Section: The Role Of Estrogen Receptors In Bone In Vivomentioning

confidence: 99%

Estrogen receptors alpha and beta in bone

Khalid

Krum

2016

Bone

224

134

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Estrogens are important for bone metabolism via a variety of mechanisms in osteoblasts, osteocytes, osteoclasts, immune cells and other cells to maintain bone mineral density. Estrogens bind to estrogen receptor alpha (ERα) and ERβ, and the roles of each of these receptors are beginning to be elucidated through whole body and tissue-specific knockouts of the receptors. In vitro and in vivo experiments have shown that ERα and ERβ antagonize each other in bone and in other tissues. This review will highlight the role of these receptors in bone, with particular emphasis on their antagonism.

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“…The treatment of young female rats with estrogen has caused a 50% reduction in the number of alveolar bone osteoclasts due to osteoclast death by apoptosis (Faloni et al. 2007; Cruzoé‐Souza et al. 2009).…”

Section: Introductionmentioning

confidence: 99%

Structural and functional changes in the alveolar bone osteoclasts of estrogen‐treated rats

et al. 2011

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This study investigated structural and functional features of apoptotic alveolar bone osteoclasts in estrogentreated rats. For this purpose, 15 female rats 22 days old were divided into three groups: Estrogen (EG), Sham (SG) and Control (CG). The rats of EG received daily intramuscular injection of estrogen for 7 days. The SG received only the oil vehicle. Maxillary fragments containing alveolar bone were removed and processed for light and transmission electron microscopy. Area (OcA) and number of nuclei (OcN) and bone resorption surface per TRAP-positive osteoclasts (BS ⁄ OC) were obtained. Vimentin, caspase-3 and MMP-9 immunoreactions, TUNEL ⁄ TRAP and MMP-9 ⁄ TUNEL combined reactions were performed. In EG, the OcA, OcN and BS ⁄ Oc were reduced. Moreover, osteoclasts showed cytoplasm immunolabelled by caspase-3 and a different pattern of vimentin expression in comparison with CG and SG. MMP-9 expression was not affected by estrogen and the TUNEL-positive osteoclasts were MMP-9-immunolabelled. In EG, ultrastructural images showed that apoptotic osteoclasts did not exhibit ruffled borders or clear zones and were shedding mononucleated portions. TRAPpositive structures containing irregular and dense chromatin were partially surrounded by fibroblast-like cells.In conclusion, the reduction in the BS ⁄ Oc may be due to reduction in OcA and OcN; these effects seem to be related to vimentin disarrangement rather than to an interference of estrogen with osteoclast MMP-9 expression. Osteoclast apoptosis involves caspase-3 activity and vimentin degradation; these cells release portions containing one apoptotic nucleus and, subsequently, undergo fragmentation, giving rise to apoptotic bodies.

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Immunohistochemical detection of estrogen receptor β in alveolar bone cells of estradiol‐treated female rats: possible direct action of estrogen on osteoclast life span

Cited by 32 publications

References 55 publications

Enhanced ERbeta immunoexpression and apoptosis in the germ cells of cimetidine-treated rats

Enhanced ERbeta immunoexpression and apoptosis in the germ cells of cimetidine-treated rats

Estrogen receptors alpha and beta in bone

Structural and functional changes in the alveolar bone osteoclasts of estrogen‐treated rats

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