Immunohistochemical detection of estrogen receptors in fish scales

Comparative analysis of a teleost skeleton transcriptome provides insight into its regulation

“…In gilthead sea bream three ERs receptors have previously been isolated and are 588 expressed in skeletal tissue, such as the scales, vertebrae and dentary bone and are 589 associated with calcium mobilisation [95,130]. The results of the present qPCR study 590 confirmed the low to undetectable levels of sbERα mRNA in skeletal tissue in the 591 gilthead sea bream.…”

Divergent responsiveness of the dentary and vertebral bone to a selective estrogen-receptor modulator (SERM) in the teleost Sparus auratus

“…As previously observed sbERa mRNA expression in sea bream calcified tissue was low to undetectable ( Fig. 2 and 3) [26] and raloxifene treatment did not modify transcript abundance. In contrast, sbERba and sbERbb transcripts were readily detected in bone and raloxifene treatment significantly (p < 0.05) up-regulated both transcripts in the dentary (Fig.…”

“…Estrogen has previously been shown in sea bream to affect scales a post-cranial dermal tissue [26]. In the present study the potential action of estrogens on the dentary and vertebral bone was assessed using the SERM raloxifene.…”

“…Fish have three estrogen receptors (ERs), one ERa and two ERb receptors [14,28] and all three occur in sea bream (Sparus auratus) scales [26] but their role and relative importance in turnover of other bony tissue is unclear. In mammals estradiol-17b (E 2 ) regulates bone homeostasis mainly through calcium anti-resorptive effects [38], both by modifying gene transcription and through rapid non-genomic actions [43].…”

Immunocytochemical Tools Reveal a New Research Field Between the Boundaries of Immunology and Reproductive Biology in Teleosts

“…Especially in the gilthead seabream, numerous antibodies have been obtained in the recent years, particularly those specific for studying the endocrine regulation of multiple biological processes such as reproduction, growth, metabolism, etc. (Abad et al, 1992;García-Ayala et al, 2003;Modig et al, 2008;Morgado et al, 2007;Picchietti et al, 2007;Pinto et al, 2009;Pirone et al, 2008;Radaelli et al, 2003Radaelli et al, , 2005Santos et al, 2001;Villaplana et al, 1997). Regarding the gilthead seabream immune response, an array of monoclonal and polyclonal antibodies have been developed for leukocyte markers (Sepulcre et al, 2002), immune molecules ( Corrales et al, 2010;García-Castillo et al, 2004;Pelegrín et al, 2004) or immunoglobulins (Picchietti et al, 2006).…”