Immunohistochemical detection of keratin, actin and type IV collagen in serial sections of methacarn-fixed breast cancer tissues.

Senzaki, Hideto; Tsubura, Airo; Shoji, Tetsuji; Okada, Hiyoshi; Takahashi, Hideki; Morii, Sotokichi

doi:10.1267/ahc.25.31

Cited by 7 publications

(3 citation statements)

References 14 publications

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“…with a single dose (50 μg/g body weight) of 5‐bromo‐2′‐deoxyuridine (BrdU; Sigma, St. Louis, MO) 1 hour before perfusion. The fixative was methacarn (6 volumes of methanol + 3 volumes of chloroform + 1 volume of glacial acetic acid; Senzaki et al, 1992). By following standard histological procedures, 5‐μm paraffin sections were placed under ultraviolet light for 2 hours to enhance the BrdU signal (Weiler et al, 1999), deparaffinized, and rehydrated.…”

Section: Methodsmentioning

confidence: 99%

Distribution of keratin 8‐containing cell clusters in mouse embryonic tongue: Evidence for a prepattern for taste bud development

Mbiene

Roberts

2003

J of Comparative Neurology

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The initiation of the morphogenesis of gustatory papillae is independent of innervation. To address the question of whether taste bud formation is associated with gustatory papilla morphogenesis, we examined developing tongues in mouse embryos from embryonic day 11 to birth. Despite the smooth morphological appearance of the lingual dorsal surface at 13 days of gestation, we observed embryonic taste bud primordia as discrete collections of cytokeratin 8-positive and elongated cells in epithelial placodes in the anterior tongue. In subsequent stages until birth, cytokeratin 8 continues to be expressed in embryonic taste buds distributed in punctuate patterns at regular intervals along rows that are symmetrically located on both sides of the median sulcus in the dorsal anterior developing tongue. Embryonic taste buds were observed in the developing circumvallate papillae from 15.5 days of gestation until birth. The dorsal epithelium of the anterior tongue is not innervated when embryonic taste buds first occur. The increased numbers of embryonic taste buds in developing fungiform papillae until birth are not correlated with the neural invasion of the epithelium. Thus, taste buds occur prenatally more likely independently of the innervation.

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Section: Methodsmentioning

confidence: 99%

Distribution of keratin 8‐containing cell clusters in mouse embryonic tongue: Evidence for a prepattern for taste bud development

Mbiene

Roberts

2003

J of Comparative Neurology

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“…At the ultrastructural level, myoepithelial-like cells have also been reported in grade I carcinomas (Hayashi et al, 1984). Finally, several reports have correlated the ultrastructural or immunoreactive presence of myoepithelial-like cells with an ultrastructurally or immunocytochemically defined basement membrane (Gould et al, 1975;Wetzels et al, 1989;Senzaki et al, 1992).…”

Section: The Function Of Normal Myoepithelial Cellsmentioning

confidence: 97%

Normal and tumor-derived myoepithelial cells differ in their ability to interact with luminal breast epithelial cells for polarity and basement membrane deposition

Guðjónsson¹,

Rønnov‐Jessen²,

Villadsen³

et al. 2002

Journal of Cell Science

430

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The signals that determine the correct polarity of breast epithelial structures in vivo are not understood. We have shown previously that luminal epithelial cells can be polarized when cultured within a reconstituted basement membrane gel. We reasoned that such cues in vivo may be given by myoepithelial cells. Accordingly, we used an assay where luminal epithelial cells are incorrectly polarized to test this hypothesis. We show that culturing human primary luminal epithelial cells within collagen-I gels leads to formation of structures with no lumina and with reverse polarity as judged by dual stainings for sialomucin, epithelial specific antigen or occludin. No basement membrane is deposited, and β4-integrin staining is negative. Addition of purified human myoepithelial cells isolated from normal glands corrects the inverse polarity, and leads to formation of double-layered acini with central lumina. Among the laminins present in the human breast basement membrane (laminin-1, -5 and -10/11), laminin-1 was unique in its ability to substitute for myoepithelial cells in polarity reversal.Myoepithelial cells were purified also from four different breast cancer sources including a biphasic cell line. Three out of four samples either totally lacked the ability to interact with luminal epithelial cells, or conveyed only correction of polarity in a fraction of acini. This behavior was directly related to the ability of the tumor myoepithelial cells to produce α-1 chain of laminin. In vivo, breast carcinomas were either negative for laminin-1 (7/12 biopsies) or showed a focal, fragmented deposition of a less intensely stained basement membrane (5/12 biopsies). Dual staining with myoepithelial markers revealed that tumor-associated myoepithelial cells were either negative or weakly positive for expression of laminin-1, establishing a strong correlation between loss of laminin-1 and breast cancer. We conclude that the double-layered breast acinus may be recapitulated in culture and that one reason for the ability of myoepithelial cells to induce polarity is because they are the only source of laminin-1 in the breast in vivo. A further conclusion is that a majority of tumor-derived/-associated myoepithelial cells are deficient in their ability to impart polarity because they have lost their ability to synthesize sufficient or functional laminin-1. These results have important implications for the role of myoepithelial cells in maintenance of polarity in normal breast and how they may function as structural tumor suppressors.

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“…Transitional epithelium, respiratory tract epithelium and glandular epithelia containing both basal and luminal cells (apocrine and eccrine sweat glands, salivary glands, mammary gland, endometrium and epididymus), as well as rete teste epithelium and rete ovarii epithelium, contain high and low molecular weight keratins in humans (ACHTSTA~ER et a]., 1985; AUMULLER et al, 1991;CZERNOBILSKY et al, 1985;KASPER and STOSIEK, 1989;LEE et al, 1990;MOLL et al, 1982;NISHIMURA et al, 1991;OSBORN and WEBER, 1983;PURKIS et al, 1990;RAMAEKERS et al, 1987;SENZAKI et al, 1992;TROYANOVSKY et al, 1989) and reactivity with antibodies raised against human high and low molecular weight keratins has been described in corresponding tissues of dogs (FERRER et SANDUSKY et al, 1991;VOS et al, 1989VOS et al, , 1992b PEASTON et al, 1992). These data are in accordance with our findings in feline tissues as those epithelial types reacted with all three antibodies employed.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Distribution of Keratin Proteins in Feline Tissues

Mulas

Monteros

Fernandez

et al. 1994

Journal of Veterinary Medicine Series A

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Summary The immunohistochemical distribution pattern of some keratin intermediate filament proteins has been analysed in a wide range of formalin fixed, paraffin embedded feline tissues using one polyclonal and two monoclonal antibodies raised against human keratins by means of the avidin‐biotin‐peroxidase complex technique. Only the epithelial and mesothelial cells were stained by the three antibodies, but differences in their corresponding staining pattern were noticed. The staining reaction of the polyclonal antibody raised against human skin keratin was found in both stratified and complex epithelia, while that of the monoclonal antibody which recognizes human keratins 8 + 18 + 19 of the Moll catalogue (NCL‐5D3) was restricted to some simple epithelia. The staining reaction of the monoclonal antibody which reacts with human keratins 5 + 8 of the Moll catalogue (RCK‐102) covered the widest spectrum of feline epithelial tissues analysed, including stratified, complex and simple epithelia. These staining patterns of feline tissues are basically similar with respect to those of corresponding tissues in other mammalian species, although some differences were also noticed and some obvious epithelial tissues were not stained. This study confirms the broad interspecies cross‐reactivity of keratin proteins antibodies and demonstrates their capability to differentiate between various types of feline epithelia and some epithelial compartments.

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Immunohistochemical detection of keratin, actin and type IV collagen in serial sections of methacarn-fixed breast cancer tissues.

Cited by 7 publications

References 14 publications

Distribution of keratin 8‐containing cell clusters in mouse embryonic tongue: Evidence for a prepattern for taste bud development

Distribution of keratin 8‐containing cell clusters in mouse embryonic tongue: Evidence for a prepattern for taste bud development

Normal and tumor-derived myoepithelial cells differ in their ability to interact with luminal breast epithelial cells for polarity and basement membrane deposition

Immunohistochemical Distribution of Keratin Proteins in Feline Tissues

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