Immunohistochemical Determination Of expression of Somatostatin Receptors Types 1, 2a, 3 and 5 in Neuroendocrine Tumors of Various Localization and Grade

Гуревич, Л. Е.; Корсакова, Н. А.; Voronkova, I A; Ашевская, В. Е.; Титов, А. Г.; Когония, Л. М.; Егоров, А. В.; Britvin, Timur; Васильев, И. А.

doi:10.18786/2072-0505-2016-44-4-378-390

Cited by 6 publications

(1 citation statement)

References 28 publications

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“…Для диагностики этой группы опухолей бальная шкала морфологической оценки злокачественности, разработаннная Weiss и соавт., не подходит, и для этого предложены критерии морфологической стратификации Lin-Weiss-Bisceglia [12]. По этим критериям опухоли в нашем исследовании были верифицированы как Таким образом, по данным большинства исследований, РССТ5 экспрессируются в АКР чаще, чем РССТ2, что отличается от нейроэндокринных опухолей различной локализации, в которых наблюдается существенное преобладание РССТ2 с интенсивностью в 2 и 3 балла [19].…”

Section: обсуждение основного результата исследованияunclassified

Somatostatin receptor expression in adrenocortical carcinomas

Voronkova

Александровна²,

Gurevch

et al. 2018

Probl Endokrinol (Mosk)

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Background. Adrenocortical carcinoma (ACC) is a rare malignant tumor characterized by an annual incidence of 0.5—2 cases per million population. Surgery is the-first line treatment for ACC. When total tumor resection is not possible due to its proliferation or progression, mitotane (o,p’DDD) is used. In this case, stabilization and partial response (as assessed by RECIST criteria) was observed only in 48.7% of cases, necessitating the search for new therapeutic targets. Objective — the study was aimed at assessing the somatostatin receptor expression in adrenocortical carcinomas and adrenal cortex tumors with uncertain malignant potential. Material and methods. Surgical material from adrenocortical tumors of 13 patients (4 males and 9 females aged from 28 to 68 years) was used. In all cases, the diagnosis was verified by morphological and immunohistochemical (IHC) studies: ACC was detected in 10 cases (including 1 case of ACC liver metastasis), oncocytic carcinoma — 1 case, oncocytoma with uncertain malignant potential — 2 cases. Morphological assessment of ACC was carried out according to Weiss criteria (for ACC tumors) and Lin—Weiss—Bisceglia criteria (for oncocytic neoplasms of the adrenal cortex). IHC study was carried out with antibodies to the spectrum of adrenal cortex-specific markers, as well as Ki-67 and somatostatin receptors 2 and 5 (SSTR2 and SSTR5). Results. The expression of SSTR2 and/or 5 was detected in 8 (61.5%) of 13 cases of ACC. Isolated SSTR2 expression was observed in 4 cases (4/13), while SSTR5 expression was observed in 6 cases (6/10). In 2 cases (2/10), co-expression of both receptor types was observed. SSTR expression was observed both in ACC and ACC liver metastasis, as well as in oncocytic ACC. Conclusion. SSTR2 and/or 5 expression in ACC tissue expands diagnostic and prognostic capabilities for this pathology.

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Section: обсуждение основного результата исследованияunclassified

Somatostatin receptor expression in adrenocortical carcinomas

Voronkova

Александровна²,

Gurevch

et al. 2018

Probl Endokrinol (Mosk)

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Peculiarities of acute myocardial infarction course and distribution of the somatostatin receptor analogue 99mTc-octreotide in the heart

Ryabov,

Syrkina,

Trusov

et al. 2023

Russ J Cardiol

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Aim. To study the relationship between the clinical course of myocardial infarction (MI) with changes in left ventricular (LV) geometry and myocardial accumulation of the somatostatin analogue 99mTc-octreotide according to cardiac single-photon emission computed tomography (SPECT).Material and methods. This pilot, cohort, prospective study included 14 patients aged 37 to 72 years with primary anterior ST-segment elevation MI. Patients underwent a general clinical examination, 3-fold determination of high-sensitivity C-reactive protein (hsCRP), echocardiography on days 1-3, cardiac SPECT with 99mTc-octreotide on days 6-7 from MI onset. Statistical analysis was performed using STATISTICA 10.0, StatSoft, USA.Results. In 10 of 14 patients, 99mTc-octreotide accumulation in LV involvement area was recorded. The accumulation analysis showed varying intensity. This made it possible to form two subgroups with high and low accumulation of 99mTc-octreotide (heart-to-cavity LV ratio more and less than 1,5, respectively).Clinical characteristics and MI course, length of hospital stay did not differ between the subgroups. Occlusion of the infarct-related coronary artery (in 5 (62,5%) vs 0 (0%) patients, p=0,016) was more common in the group with increased versus decreased accumulation of 99mTc-octreotide, respectively.Laboratory analysis showed a significant increase in hsCRP upon admission in patients with increased vs decreased accumulation of 99mTc-octreotide — 31,6 mg/l (10,45-42,05) vs 6,5 mg/l (3,2-11,8), p=0,043, respectively.A significant increase in end-diastolic and end-systolic LV volumes was established. There were an increase in LV sphericity index and local contractility disruption index along with a decrease in LV ejection fraction in the subgroup with high vs reduced accumulation of 99mTc-octreotide (47,5% (45,5-51,5) vs 57,5% (54-59), p=0,020) in the damage zone, respectively. In addition, a predictive relationship between the increase in LV end-diastolic volume and the severity of 99mTc-octreotide accumulation was found (R2=0,601, F-ratio=16,562, p=0,002).Conclusion. An increase in hsCRP and early initiation of LV remodeling after primary anterior ST-segment elevation MI is accompanied by a pronounced accumulation of 99mTc-octreotide in the damage zone, detected using cardiac SPECT, as a result of systemic and local, in our opinion, macrophage-mediated post-infarction inflammation.

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Cyphetrylin effect on somatostatin receptors expression in transplantable Ca-755 mice tumor

Киселева

Борисова

Delektorskaya

et al. 2022

Rossijskij bioterapevtičeskij žurnal

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Background. Expression evaluation of somatostatin receptors (SSTRs) in tumor cells is necessary for the reasonable use of therapy directed at such receptors.Aim. The affinity determination of the original analogue of somatostatin cyphetrylin for SSTRs of transplanted mice mammary adenocarcinoma Ca-755.Materials and methods. Cyphetrylin was synthesized in the Chemical Synthesis Laboratory of the Research Institute of Experimental Diagnostics and Therapy of Tumors, N.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of Russia. Cyphetrylin in tablet form was administered orally at a therapeutic dose of 10 mg/kg for 7 days to female F1 (C57Bl/6 × DBA/2) tumor-grafted Ca-755 mice. Animals of the control group were not administreted with сyphetrylin. Tumor tissue samples were obtained from animals on the 9th and 14th days after Ca-755 transplantation and sent for immunohistochemical study, which was performed on serial paraffin sections by the immunoperoxidase method using primary antibodies to various types of SSTRs.Results. The high frequency of positive expression of SSTR1, SSTR2, and SSTR5 (in 80, 100 and 100 % of tumor samples, respectively) was shown in tumor samples of the control group animals. As a result of cyphetrylin introduction in tumor samples obtained on the 9th day after Ca-755 inoculation, a change in the tumor receptor status was found towards a decrease in the level of expression of SSTR2 (80 % of samples) and SSTR5 (80 % of samples); SSTR1 expression did not change (80 % of samples). Compared to the controlled, in tumor samples after cyphetrylin administration, obtained on day 14 from Ca-755 transplantation, a decrease in the expression level of SSTR2 (80 % of samples), SSTR1 and SSTR5 (60 % of samples for SSTR each type) was noted, due to cyphetrylin binding to SSTRs of tumor cells. The receptors SSTR3 and SSTR4 did not show a high level of expression in the studied Ca-755 tumor samples.Immunohistochemical staining of Ca-755 cells with antibodies to SSTRs showed a tendency to reduction of antigen-positive cells number from 15–50 % in control to 10–40 % on day 9 after Ca-755 transplantation and 10–30 % on day 14 after Ca-755 transplantation.Conclusion. The data obtained indicates the presence in mice transplanted mammary adenocarcinoma Ca-755 of SSTR1, SSTR2 and SSTR5 high level expression due to the binding to which the direct cyphetrylin antitumor effect is realized.

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Immunohistochemical Determination Of expression of Somatostatin Receptors Types 1, 2a, 3 and 5 in Neuroendocrine Tumors of Various Localization and Grade

Cited by 6 publications

References 28 publications

Somatostatin receptor expression in adrenocortical carcinomas

Somatostatin receptor expression in adrenocortical carcinomas

Peculiarities of acute myocardial infarction course and distribution of the somatostatin receptor analogue 99mTc-octreotide in the heart

Cyphetrylin effect on somatostatin receptors expression in transplantable Ca-755 mice tumor

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