Immunohistochemical diagnosis of mucous membrane pemphigoid

Shimanovich, Iakov; Nitz, Julia Marie; Witte, Mareike; Zillikens, Detlef; Rosé, Christian

doi:10.1111/jop.12732

Cited by 14 publications

(24 citation statements)

References 14 publications

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“…The formalin‐fixed, paraffin‐embedded jejunum was used (Canene‐Adams 2013) for the diagnosis of SIgA + cells using and immunohistochemical method according to the procedures of Shimanovich et al . (2018). The average optical density (AOD) of SIgA + cells in the jejunum mucosa was detected using the ImageJ analysis system.…”

Section: Methodsmentioning

confidence: 99%

Deposition of resistant bacteria and resistome through FMT in germ‐free piglets

Sun

Chen

Ding

et al. 2021

Lett Appl Microbiol

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Significance and Impact of the Study: Faecal microbiota transplantation (FMT) is expecting to be a potentially useful tool for promoting intestinal health and improving outcomes in farm animals. This study showed a critical period in which early-life microbial exposure could have a profound influence on the enrichment of gut microbiota as well as transmission of antibiotic-resistant genes between pigs through swine faeces, which corresponds to the pattern of antibiotic consumption in the pig farm. Therefore, in the future, it is necessary to deeply understand the sources of resistance gene acquisition and be more cautious with FMT in animals.

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Section: Methodsmentioning

confidence: 99%

Deposition of resistant bacteria and resistome through FMT in germ‐free piglets

Sun

Chen

Ding

et al. 2021

Lett Appl Microbiol

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“…For DIF, fresh frozen tissue is preferred compared with formalin‐fixed paraffin‐embedded (FFPE) tissue, but DIF on trypsin digestion of formalin‐fixed sections can be an alternative method when fresh frozen tissue is unavailable . In addition, if only FFPE tissues are available, C3d or C4d immunohistochemistry (IHC) has also been considered as a valuable diagnostic tool for bullous pemphigoid (BP) and MMP . IHC for C4d or IgG4 may be diagnostically useful in pemphigus, and C4d IHC can be helpful in the diagnosis of pemphigoid gestationis .…”

Section: Immunological Methodsmentioning

confidence: 99%

“…[4][5][6] In addition, if only FFPE tissues are available, C3d or C4d immunohistochemistry (IHC) has also been considered as a valuable diagnostic tool for bullous pemphigoid (BP) and MMP. [7][8][9][10][11] IHC for C4d or IgG4 may be diagnostically useful in pemphigus, 10,12 and C4d IHC can be helpful in the diagnosis of pemphigoid gestationis. 13 A reliable technique of IHC for IgA and IgG on FFPE tissue needs to be established to obtain higher sensitivity.…”

Section: Direct Immunofluorescencementioning

confidence: 99%

Immunological methods for the diagnosis of oral mucosal diseases

Sun

Zhong

et al. 2019

Br J Dermatol

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“…13 Two studies found that age and sex did not have an impact on IHC positivity, 11,13 and 3 investigations reported partly contradictory results regarding the impact of positive immunoserology on the sensitivity of IHC (Table 1). 11,13,15 Figure 2 shows the methodological analysis of the included studies based on QUADAS-2, with outcomes resembling bias risk and applicability. Although all studies used an acceptable reference standard (ie, DIF) and low applicability concerns were found consistently across studies, most issues were observed in the risk of bias for patient selection and the index test (ie, IHC).…”

Section: Characteristics and Qualities Of Included Studiesmentioning

confidence: 99%

Immunoglobulin and Complement Immunohistochemistry on Paraffin Sections in Autoimmune Bullous Diseases: A Systematic Review and Meta-analysis

Kasperkiewicz

Lai

Kim

et al. 2020

The American Journal of Dermatopathology

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Immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded tissue has been proposed as a potential tool in the diagnosis of autoimmune bullous diseases (AIBDs) in lieu of standard direct immunofluorescence (DIF) microscopy. To comprehensively determine the diagnostic accuracy of immunoglobulin and complement IHC for diagnosis of AIBDs, we conducted a systematic review and multivariate Bayesian model-based meta-analysis of the literature. Quality and heterogeneity assessment of studies was performed using the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) checklist and the I 2 index, respectively. Electronic searches using PubMed from April 1964 to July 2020 identified 14 articles meeting predetermined inclusion and exclusion criteria. Median sensitivities with 95% credible intervals in pemphigus and pemphigoid were 0.24 (0.01-0.89) and 0.22 (0.02-0.77) with immunoglobulin G (IgG), 0.77 (0.39-0.95) and 0.25 (0.02-0.85) with IgG4, 0.11 (0.02-0.32) and 0.86 (0.56-0.98) with C3d, and 0.84 (0.56-0.97) and 0.75 (0.37-0.94) with C4d, respectively. Specificities were 1.00 (0.00-1.00) with IgG, 0.98 (0.89-1.00) with IgG4, 0.99 (0.97-1.00) with C3d, and 0.99 (0.97-1.00) with C4d. The risk of bias and heterogeneity among studies was a serious problem, decreasing the level of evidence. Our work suggests that, in selected cases, paraffin-based IHC may be a helpful procedure to screen for AIBDs, especially when specialized laboratories and/or biopsy specimens for DIF do not exist. Nevertheless, more studies with a refined quality design are needed to explore the true usefulness of this diagnostic method in AIBDs.

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Immunohistochemical diagnosis of mucous membrane pemphigoid

Cited by 14 publications

References 14 publications

Deposition of resistant bacteria and resistome through FMT in germ‐free piglets

Deposition of resistant bacteria and resistome through FMT in germ‐free piglets

Immunological methods for the diagnosis of oral mucosal diseases

Immunoglobulin and Complement Immunohistochemistry on Paraffin Sections in Autoimmune Bullous Diseases: A Systematic Review and Meta-analysis

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