Immunohistochemical evaluation of CD117 in mast cell of aggressive periodontitis

Mazreah, Siavash Azizi; Shahsavari, Maryam; Kalati, Parvin Arbabi; Mazreah, Homa Azizi

doi:10.4103/jisp.jisp_243_19

Cited by 5 publications

(4 citation statements)

References 22 publications

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“…The role of MCs in fibrosis is controversial, and MCs are known to release mediators that promote fibrogenesis [6,18,19]. Our findings are consistent with previous studies [3,5,17]. Our findings disagree with those of Rajendran et al, who observed similar values of the mean vascular density in various grades of OSMF, which were marginally lower than those of normal oral mucosa [20].…”

Section: Angiogenesis Mast Cells and Osmfsupporting

confidence: 71%

“…Mast cells (MCs) are indistinguishable components of connective tissue and other inflammatory cells and are associated with several inflammatory disorders, including lichen planus, gingivitis, pulpitis, and aggressive periodontitis [3,4]. MCs are known to cause a burning sensation in OSMF, and more mast cells are detected in the early stages of OSMF than in the advanced stages of OSMF [5].…”

Section: Introductionmentioning

confidence: 99%

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The Immunolocalization of Mast Cells in the Pathology of Oral Submucous Fibrosis

Gupta¹,

Sharma²,

Banerjee³

et al. 2023

Cureus

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BackgroundOral submucous fibrosis (OSMF) is a prevalent precancerous condition of the oral cavity and an ambiguity to clinicians because of its indistinguishable etiopathogenesis. Previous studies could not establish a definite role of mast cells (MCs) in the fibrosis of stroma. The present study was done to study the histopathological changes in OSMF and to determine the association of mast cells (MCs) and their degranulated components with vascularity. MethodsA retrospective case-control study involved 40 cases of various histopathological grades of OSMF and was compared with 10 cases of normal buccal mucosa by using a cluster of differentiation 117 (CD117) kit for the identification of MCs and Masson's trichrome stain to study the number of blood vessels (BVs). ResultsThe present study indicated that advanced cases of OSMF had keratinized epithelium with atrophic changes and moderate to advanced fibrosis of stroma with the involvement of underlying muscles. The MC density and the number of blood vessels were progressively reduced in OSMF as the grade advanced compared to healthy controls. ConclusionAn increase in the mast cell density in the initial stages of OSMF suggests their definite role in the initiation of fibrosis and secondary changes to the epithelium such as atrophy.

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Section: Angiogenesis Mast Cells and Osmfsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

The Immunolocalization of Mast Cells in the Pathology of Oral Submucous Fibrosis

Gupta¹,

Sharma²,

Banerjee³

et al. 2023

Cureus

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“…Żychowska et al 32 also identified c-kit in a cutaneous variant of lichen planus, but without differences in comparison to normal tissues. Likewise, a study by Mazreah et al 33 addressed expression levels of c-kit+ mast cells in periodontitis and found no difference in comparison to normal tissue. A study that analyzed the impact of c-kit+ mast cells in oral squamous cell carcinoma found that the presence of mast cells was associated with reduced disease recurrence.…”

Section: Discussionmentioning

confidence: 99%

Mast cells in oral lichen planus and oral lichenoid lesions related to dental amalgam contact

NORONHA,

SOUTO,

FELIX

et al. 2024

Braz. oral res.

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The aim of this study was to analyze the expression of mast cell markers toluidine blue, c-kit, and tryptase and presence of mononuclear inflammatory cells in oral lichen planus (OLP) and oral lichenoid lesions related to dental amalgam. Nineteen specimens of OLP, OLLC, and healthy oral mucosa were selected. Mononuclear inflammatory cells were analyzed. Histochemical and immunohistochemical analyses were performed using toluidine blue, anti-c-kit and anti-tryptase reagents, and the results were quantified in areas A and B of connective tissue. Mast cells of all OLP and OLLC samples were positive for toluidine blue, c-kit, and tryptase. The density of toluidine blue+, c-kit+ and tryptase+ mast cells was higher in tissue with OLP and OLLC compared with healthy controls (p < 0.05).No difference was noted in mast cells density between OLP and OLLC (p > 0.05). The density of tryptase+ mast cells was higher in the subepithelial region (area A) than the region below it (Area B) in OLLC (p = 0.047). The mononuclear inflammatory cell density was higher in OLLC compared to OLP, but without statistical significance (p > 0.05). A positive statistical correlation was found between mononuclear immune cells and density of c-kit+ and tryptase+ mast cells in OLP (r = 0.943 and r = 0.886, respectively). Our data demonstrate that the etiopathogenesis process of OLP and OLLC modulates the expansion and degranulation of mast cells; mast cells density, however, was similar between OLP and OLLC. The distribution of mast cells appears to vary along the lamina propria.

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“…Aberg з спів. (2020) збудником при ранньому розвитку пародонтиту є Aggregatibacter (Actinobacillus) actinomycetemcomitans і Porphyromonas gingivalis, які виділяють з вмісту пародонтальних кишень [14]. Дані мікроорганізми виробляють протеолітичні ферменти -колагенази, які руйнують тканини пародонту.…”

Section: епідеміологія і етіопатогенетичні фактори розвитку агресивни...unclassified

Epidemiology and Etiopathogenetic Factors of the Development of Aggressive Forms of Periodontitis

Bandrivska¹,

Lysokon²

2023

Ukr. ž. med. bìol. sportu

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The purpose of the work was to study the etiology and main etiopathological factors of the development of aggressive forms of periodontitis based on the analysis of additional pathogenetic aspects of mineral metabolism disorders according to modern literature. Materials and methods. The bibliosemantic method was used to clarify the state of the problem, study the analysis of the results of previous scientific research based on literature sources and electronic resources. Results. Periodontitis is the most widespread disease among all periodontal pathologies (chronic generalized periodontitis occurs in 90% of cases). Until recently, aggressive forms of periodontitis were not given due attention, since according to epidemiological studies by S. Renvert and M. Wikstom (1996), in the general structure of periodontal diseases, aggressive forms of periodontitis occur with a frequency of 5–10%. To understand the mechanisms of the development of aggressive forms of periodontitis, attention is paid to their development in children and adolescents. Clinical and radiological examinations of children and adolescents showed different frequency of aggressive forms of periodontitis (localized juvenile periodontitis and generalized juvenile periodontitis): in the USA – 2.27%, in Denmark – 0.1%, in Finland – 0.1%, in Nigeria – 0.8%, in Brazil – 0.3%, in England – 0.17%, in Chile – 0.32%. Studies have shown that aggressive forms of periodontitis are equally common in children and adolescents, regardless of gender. At the same time, localized juvenile periodontitis is 3 times more common in boys, not the same for girls. A new and rather promising vector of periodontology is the study of the role of juvenile changes in the bone skeleton on the development and course of periodontal diseases. The development of mineral metabolism disorders in aggressive forms of periodontitis in different age groups requires further study, while the data obtained by molecular geneticists become relevant for understanding the possible ways of research. Considering the fact that the jaw bones are a component of the skeleton, the strength of which can be affected by various exogenous and endogenous factors, in particular genetic ones, the main direction of searching for candidate sensitivity genes is conducted among the markers of bone metabolism disorders. Of many candidate genes that determine bone mineral density, the most well-known is the vitamin D receptor gene. Conclusion. The analysis of the available literature showed that the study of the role of mineral and bone metabolism disorders in the emergence and development of aggressive forms of periodontitis has a wide scientific perspective, and obtaining new data on the state of systemic regulatory factors and their effects on the local mechanisms of periodontal tissue damage will allow not only to improve the diagnosis of various forms, but also to increase the effectiveness of the treatment of this pathology

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Immunohistochemical evaluation of CD117 in mast cell of aggressive periodontitis

Cited by 5 publications

References 22 publications

The Immunolocalization of Mast Cells in the Pathology of Oral Submucous Fibrosis

The Immunolocalization of Mast Cells in the Pathology of Oral Submucous Fibrosis

Mast cells in oral lichen planus and oral lichenoid lesions related to dental amalgam contact

Epidemiology and Etiopathogenetic Factors of the Development of Aggressive Forms of Periodontitis

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