Immunohistochemical Evaluation of Vascular Endothelial Growth Factor (VEGF) in Splenic Hemangiomas and Hemangiosarcomas in Dogs

Campos, Andressa Gianotti; Campos, Juliana Álvares Duarte Bonini; Sanches, Daniel Soares; Dagli, M.L.; Matera, Júlia Maria

doi:10.4236/ojvm.2012.24030

Cited by 9 publications

(8 citation statements)

References 14 publications

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“…Restucci et al (2003), reported that they found VEGF expression to be higher in canine seminomas compared to the control group and reported that it was a useful criterion regarding malignancy and growth potential in seminomas. Campos et al (2012) reported that VEGF levels increase significantly in hemangiosarcomas and are associated with malignant vascular proliferation. Martano et al (2016) reported that VEGF expressions were significantly increased in neoplastic tissues compared to normal tissues in a study on oral squamous cell carcinomas of dogs.…”

Section: Discussionmentioning

confidence: 99%

A case of papillary and infiltrative urothelial carcinoma of the urinary bladder in a terrier dog

Akçakavak¹,

ÇELİK

Uzunlu

et al. 2023

Journal of Advances in VetBio Science and Techniques

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Urothelial carcinoma are malignant tumours originating from the epithelial layer of the urinary bladder. In this instance, a case of infiltrative type urothelial carcinoma in the urinary bladder of a 2-year-old terrier dog was defined histopathologically and immunohistochemically. The material of the presented case consisted of urinary bladder tissue samples that were surgically extirpated from a two-year-old terrier-breed female dog that applied to the Department of Surgery of the Faculty of Veterinary Medicine, Selcuk University with the complaint of hematuria. The tumour brought to the pathology laboratory was 11*10*12 cm in size and had finger-shaped extensions. Its outer surface was rough and hemorrhagic. Tissues were fixed in 10% buffered formalin and paraffin blocks were obtained by going through the necessary routine follow-up procedures. Afterwards, sections were taken and subjected to Hematoxylin-Eosin, Masson's Trichrome and immunohistochemical staining. As a result of the pathological and immunohistochemical examinations of the tumoral tissue samples taken from the urinary bladder of a two-year-old terrier dog, the diagnosis of infiltrative type urothelial carcinoma was reached, and the case was discussed with the information provided by the literature. In addition, immunohistochemically, intense PCNA and VEGF staining has been associated with malignancy.

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Section: Discussionmentioning

confidence: 99%

A case of papillary and infiltrative urothelial carcinoma of the urinary bladder in a terrier dog

Akçakavak¹,

ÇELİK

Uzunlu

et al. 2023

Journal of Advances in VetBio Science and Techniques

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“…The factor binds to high-affinity receptors belonging to the tyrosine kinase family. Its increased expression has been confirmed in several neoplastic tumours in animals as well as in cell cultures (5,6).…”

Section: Introductionmentioning

confidence: 88%

“…Neoangiogenesis involves formation of new blood vessels from the vascular endothelium of pre-existing blood vessels. The process of vascular development plays a key role in the neoplastic process, and is a pre-requisite for tumour growth and for development of metastases (6,34).…”

Section: Introductionmentioning

confidence: 99%

Usefulness of immunohistochemical indicators for diagnosis and prognosis of poorly differentiated tumours

Kandefer-Gola

Nowak

Ciaputa

et al. 2016

Journal of Veterinary Research

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Immunohistochemical studies have become an indispensable element of establishing the correct histopathological diagnosis of poorly differentiated lesions, proving particularly suitable, and occasionally indispensable, for diagnosis of poorly differentiated neoplastic tumours. Knowledge of the mechanism of action and normal reaction of individual proteins is required in selection of the antibody pattern for a given tissue and in evaluation of the obtained results. This paper aims to promote the application of immunohistochemical techniques in routine diagnosis, especially in cases of poorly differentiated or undifferentiated tumours.

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“…The identified tumour tissue was punched out using a disposable punch biopsy (Punch biopsy, 2 mm, Kai Medical, Solingen, Germany). A total of 20 punches (one of each tissue sample) were re‐embedded in one paraffin tissue block (Leica EG 1160 paraffin embedding station) along with two internal positive controls [canine ASAC (PDGFR), canine granulomatous tissue, (VEGFR)] for which positive expression of VEGFR, PDGFR‐α and PDGFR‐β has already been described …”

Section: Methodsmentioning

confidence: 99%

Expression of VEGFR and PDGFR‐α/‐βin 187 canine nasal carcinomas

Gramer

Killick

Scase

et al. 2016

Vet Comparative Oncology

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Radiotherapy represents the standard of care for intranasal carcinomas. Responses to tyrosine kinase inhibitors (TKIs) have been reported but data on expression of target receptor tyrosine kinases (rTKs) is limited. This study characterizes the expression of vascular endothelial growth factor receptor (VEGFR), platelet-derived growth factor receptor (PDGFR)-α and PDGFR-β in canine intranasal carcinomas. Histological samples from 187 dogs were retrieved. Immunohistochemistry was performed using commercially available antibodies. Expression of rTKs was classified into weak, moderate or intense and additionally recorded as cytoplasmic, membranous, cytoplasmic-membranous, nuclear or stromal. VEGFR was expressed in 158 dogs with predominantly moderate expression (36.9%) and a cytoplasmic-membranous expression pattern (70.9%). PDGFR-α was detected in 133 with predominantly weak expression (57.9%) and cytoplasmic pattern (87.9%). PDGFR-β was identified in 74 patients with a predominantly moderate expression (17.6%) and cytoplasmic expression pattern (63.5%). Co-expression of rTKs was common. These results confirm expression of VEGFR, PDGFR-α and PDGFR-β in canine intranasal carcinomas and support the utility of TKIs.

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Immunohistochemical Evaluation of Vascular Endothelial Growth Factor (VEGF) in Splenic Hemangiomas and Hemangiosarcomas in Dogs

Cited by 9 publications

References 14 publications

A case of papillary and infiltrative urothelial carcinoma of the urinary bladder in a terrier dog

A case of papillary and infiltrative urothelial carcinoma of the urinary bladder in a terrier dog

Usefulness of immunohistochemical indicators for diagnosis and prognosis of poorly differentiated tumours

Expression of VEGFR and PDGFR‐α/‐βin 187 canine nasal carcinomas

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