Immunohistochemical expression of CD44 in thyroid gland lesions

Abstract

BACKGROUND: CD44 is a polymorphic family of cell surface proteoglycans and glycoproteins implicated in cell-to-cell and cell-to-matrix adhesion interactions and tumor metastasis. Its expression appears to be an indicator of invasive and metastatic behavior in carcinomas. The purpose of our study is to investigate the immunohistochemical expression of CD44 protein in thyroid lesions and its association to other histopathological parameters. METHODS: Samples from thyroid lesions were obtained from 40 patients treated in our hospital. The material consisted of 5 cases of multinodular goiter, 7 cases of thyroiditis (Hashimoto type), 5 cases of follicular adenoma, 4 cases of Hurthle cell tumor, 15 cases of thyroid carcinoma (11 papillary carcinomas, and 4 myeloid tumors), and 4 cases of normal thyroid tissue. Immunostaining was performed using the Ventana ES automated immunostainer. A monoclonal antibody was used and avidin-biotin method was applied to paraffin-embedded samples. A membranous immunostaining pattern was considered positive. RESULTS: CD44 expression was detected in three adenomas (60%), mostly of follicular type, and in eight carcinomas (72%). The CD44 immunostaining was especially apparent in papillary type of carcinomas which showed high expression. In normal thyroid tissue, a reduced CD44 expression was observed. CONCLUSION: The results of our study indicate that deregulated expression of CD44 contributes to the ability of thyroid carcinomas for invasion and metastasis and may constitute a prognostic factor for malignant biological behavior

Marcadores De Cáncer Stem Cells Y Transición Epitelio Mesenquimal Como Factores De Agresividad Y Progresión en Pacientes Jóvenes Con Cáncer De Tiroides

“…Respecto a la presencia de células tumorales CD44+, se evidenció que su presencia se asocia con acortamiento de la sobrevida libre de progresión (OR = 2,96) en CPT 26 . Por otro lado, Kiziridou et al 27 , describe que la inmunotinción de CD44 es, especialmente, aparente en CPT en contraste con otras histología y tejido normal, constituyendo un posible factor pronóstico de comportamiento maligno. También se ha visto que la detección de células tumorales CD44+ y/o CD24-tienen implicancias pronósticas en CPT.…”

Can We Predict Differentiated Thyroid Cancer Behavior? Role of Genetic and Molecular Markers

“…The immunohistochemical score of CD44 v6 protein staining is significantly higher in PTC specimens than in benign thyroid nodules or adjacent normal thyroid tissue [ 159 , 160 ]. PTC with lateral LNM showed more intensive CD44 v6 staining than without metastases.…”