Immunohistochemical Expression of Heparanases 1 and 2 in Benign Tissue and in Invasive Neoplasia of the Endometrium: A Case-Control Study

Filho, Roney Cesar Signorini; Focchi, Gustavo Rubino de Azevedo; Theodoro, Therésè Rachell; Pinhal, Maria Aparecida da Silva; Nicolau, Sérgio Mancini

doi:10.1097/igc.0000000000000329

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…Some authors have described an increased expression of HPSE2 in tumor tissues [10], [11]. HPSE2 can also modulate the catalytic activity of HPSE [12], [13].…”

Section: Discussionmentioning

confidence: 99%

Extracellular matrix alterations in the Peyronie’s disease

Watanabe

Theodoro

Coelho

et al. 2017

Journal of Advanced Research

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“…Some authors have described an increased expression of HPSE2 in tumor tissues [10], [11]. HPSE2 can also modulate the catalytic activity of HPSE [12], [13].…”

Section: Discussionmentioning

confidence: 99%

Extracellular matrix alterations in the Peyronie’s disease

Watanabe

Theodoro

Coelho

et al. 2017

Journal of Advanced Research

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“…In this present study, the finding of increased HPSE2 expression in tumoral tissues in patients with lymph node metastases from CRC, compared to the HPSE2 expression in tumor tissue obtained from patients without lymph node metastases suggests that this protein may be related to molecular mechanisms of invasion and migration of tumor cells. Data from the literature have already proven a differential expression of HPSE2 in the cervix, endometrium, and thyroid cancer [38][39][40]. The fact that increased HPSE2 expression is directly related to tumors of patients with lymph node metastases possibly indicates that the HPSE2 expression might be useful to evaluate lymph node metastasis events.…”

Section: Discussionmentioning

confidence: 99%

“…CC-BY 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprint this version posted January 5, 2021. ; https://doi.org/10.1101/2021.01.05.425386 doi: bioRxiv preprint and thyroid cancer [38][39][40]. The fact that increased HPSE2 expression is directly related to tumors of patients with lymph node metastases possibly indicates that the HPSE2 expression might be useful to evaluate lymph node metastasis events.…”

Section: Discussionmentioning

confidence: 99%

Extracellular matrix changes in colorectal carcinoma and correlation with lymph node metastasis

Theodoro

Serrano

Turke

et al. 2021

Preprint

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The process of proliferation and invasion of tumor cells depends on changes in the extracellular matrix (ECM) through the activation of enzymes and alterations in the profile of ECM components. We aimed to investigate the mRNA and protein expression of ECM components such as heparanase (HPSE), heparanase-2 (HPSE2), matrix metalloproteinase-9 (MMP-9), and syndecan-1 (SYND1) in neoplastic and non-neoplastic tissues of patients with colorectal carcinoma (CRC). It is a cross-sectional study in which twenty-four adult patients that had CRC were submitted to resection surgery. We analyzed the expression of HPSE, HPSE2, MMP-9, and SYND1 by quantitative RT-PCR and immunohistochemistry. Differing from most of the studies that compare the mRNA expression between tumor samples and non-neoplastic tissues, we decided to investigate whether variations exist in the expression of the ECM components between the affected tissue and nontumoral tissue collected from the same patient with CRC. We removed both tissue samples immediately after the surgical resection of CRC. The data showed higher mRNA and protein expression of HPSE2 (P = 0.0058), MMP-9 (P = 0.0268), and SYND1 (P = 0.0002) in tumor samples compared to the non-neoplastic tissues, while there was only an increase in the level of HPSE protein in tumor tissues. A greater expression of HPSE2 was observed in patients with lymph node metastasis (P = 0.048), suggesting that such protein can be a marker of lymph node metastasis in CRC.

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“…A previous study reported that the L1CAM [386], HSD17B2 [387], GRP (gastrin releasing peptide) [388], FABP4 [389], SOX6 [390]. MMP12 [391], APOD (apolipoprotein D) [392], LAG3 [393], CST1 [394], FLT1 [395], DHCR24 [396], PRL (prolactin) [397], WNT5A [398], TIMP3 [399], CD24 [400], LHCGR (luteinizing hormone/choriogonadotropin receptor) [401], MMRN1 [402], CD4 [403], ADAMTS5 [404], ADAMTS1 [405], PADI2 [406], MARK1 [407], KL (klotho) [408], PLAU (plasminogen activator, urokinase) [409], SOX8 [410], CRABP2 [411], PTPRD (protein tyrosine phosphatase receptor type D) [412], EPCAM (epithelial cell adhesion molecule) [413], IRX2 [414], SEMA3B [415], CYP1A1 [416], PDGFD (platelet derived growth factor D) [417], LEPR (leptin receptor) [418], APOE (apolipoprotein E) [419], CASP1 [420], MGLL (monoglyceride lipase) [421], NID1 [422], ABCG2 [423], ACE (angiotensin I converting enzyme) [424], PGR (progesterone receptor) [425], HPSE2 [426], LMTK3 [427], ALPP (alkaline phosphatase, placental) [428], EGFL6 [429], CACNA2D3 [430], MCTP1 [431], HKDC1 [432], S100A4 [433], MACC1 [434], MMP3 [435], FGFR2 [436], IL33 [437], FOXC2 [438], ITGA7 [439], EFEMP1 [440], GATA6 [441], BHLHE41 [442], TCF21 [443], GDF10 [444], NKX3-1 [445], AKR1C3 [446], SGK1 [447], RAP1GAP [448], FLI1 [449], NOX4 [450], SERPINE2 [451], IGSF9 [452], IGF2BP1 [453], SALL4 [454], VDR (vitamin D receptor) [455], CELSR2 [456],...…”

Section: Discussionmentioning

confidence: 99%

Screening and identification of key biomarkers associated with endometriosis using bioinformatics and next generation sequencing data analysis

Vastrad,

Vastrad

2024

Preprint

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Endometriosis is a common cause of endometrial-type mucosa outside the uterine cavity with symptoms such as painful periods, chronic pelvic pain, pain with intercourse and infertility. However, the early diagnosis of endometriosis is still restricted. The purpose of this investigation is to identify and validate the key biomarkers of endometriosis. Next generation sequencing (NGS) dataset GSE243039 was obtained from the Gene Expression Omnibus (GEO) database, and differentially expressed genes (DEGs) between endometriosis and normal control samples were identified. After screening of DEGs, gene ontology (GO) and REACTOME pathway enrichment analyses were performed. Furthermore, a protein-protein interaction (PPI) network was constructed and modules were analysed using the Human Integrated Protein-Protein Interaction rEference (HIPIE) database and Cytoscape software, and hub genes were identified. Subsequantely, a network between miRNAs and hub genes, and network between TFss and hub genes were constructed using the miRNet and NetworkAnalyst tool, and possible key miRNAs and TFs were predicted. Finally, receiver operating characteristic curve (ROC) analysis was used to validate the hub genes. A total of 958 DEGs, including 479 up regulated genes and 479 down regulated genes, were screened between endometriosis and normal control samples. GO and REACTOME pathway enrichment analyses of the 958 DEGs showed that they were mainly involved in multicellular organismal process, developmental process, signaling by GPCR and muscle contraction. Further analysis of the PPI network and modules identified 10 hub genes, including VCAM1, SNCA, PRKCB, ADRB2, FOXQ1, MDFI, ACTBL2, PRKD1, DAPK1 and ACTC1. Possible target miRNAs, including hsa-mir-3143 and hsa-mir-2110, and target TFs, including TCF3 and CLOCK, were predicted by constructing a miRNA-hub gene regulatory network and TF-hub gene regulatory network. This investigation used bioinformatics techniques to explore the potential and novel biomarkers. These biomarkers might provide new ideas and methods for the early diagnosis, treatment, and monitoring of endometriosis.

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Immunohistochemical Expression of Heparanases 1 and 2 in Benign Tissue and in Invasive Neoplasia of the Endometrium: A Case-Control Study

Cited by 6 publications

References 25 publications

Extracellular matrix alterations in the Peyronie’s disease

Extracellular matrix alterations in the Peyronie’s disease

Extracellular matrix changes in colorectal carcinoma and correlation with lymph node metastasis

Screening and identification of key biomarkers associated with endometriosis using bioinformatics and next generation sequencing data analysis

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