Immunohistochemical Expression of RARα, RARβ, and Cx43 in Breast Tumor Cell Lines After Treatment With Lycopene and Correlation With RT-QPCR

Chalabi, Nasséra; Delort, Laëtitia; Satih, Samir; Déchelotte, P.; Bignon, Yves‐Jean; Bernard‐Gallon, Dominique

doi:10.1369/jhc.7a7185.2007

Cited by 25 publications

(13 citation statements)

References 21 publications

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“…Slides Slides were then counterstained with hematoxylin for 3 min, rinsed in distilled water, and placed on coverslips with an aqueous Faramount mounting media (dako, Glostrup, denmarck). The primary polyclonal antibody was omitted and replaced with PBS as a negative control (39).…”

Section: Methodsmentioning

confidence: 99%

BRCA1, BRCA2, AR and IGF-I expression in prostate cancer: Correlation between RT-qPCR and immunohistochemical detection

Rabiau

Déchelotte²,

Adjakly³

et al. 2011

Oncol Rep

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Abstract. Identification and characterization of biomarkers in prostate cancer are important for improving the diagnosis. The aim of this study was to determine differences in the expression of 4 genes according to the stage of malignancy in prostate cancer. We analyzed BRCA1, BRCA2, androgen receptor (AR) and IGF-I gene expression in a cohort of 98 prostate biopsies. We used TaqMan RT-qPCR for mRNA detection, and correlation with proteins was performed using immunohistochemistry. Among the 98 studied prostate biopsies, high heterogeneity in the expression of the 4 genes was detected among the different histological types. However, down-regulation of BRCA1 and BRCA2 mRNA was detected, particularly in the normal tissues. The expression of AR was dependent on the stage of the tumor. The IGF-I gene was specifically expressed in the tumor tissues. Upon comparison between protein and mRNA expression for BRCA1, BRCA2 and AR, we obtained a trend; however, this did not achieve statistical significance. Regarding IGF-I, a correlation between mRNA expression and staining intensity of the protein was found to be significant (p<0.012). The AR biomarker was found to be slightly correlated with the prostate cancer diagnosis (p=0.013). AR was found to be decreased in the tumors with a 43% sensitivity and 90% specificity. The relative risk of 2.05 (1.13-3.69) indicated a 2-fold higher chance of cancer occurence when AR was ≤0.206.

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Section: Methodsmentioning

confidence: 99%

BRCA1, BRCA2, AR and IGF-I expression in prostate cancer: Correlation between RT-qPCR and immunohistochemical detection

Rabiau

Déchelotte²,

Adjakly³

et al. 2011

Oncol Rep

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“…Lycopene appears to have significant benefits in reducing the risk of developing chronic diseases such as cancer and cardiovascular disease [9,10]. The benefits of lycopene could be attributed to not only its antioxidative action but also its capacity to upregulate Cx43 expression in cancer cells [11,12]. The over-expression of Cx43 leads to decreased proliferation in melanoma cells [13].…”

Section: Introductionmentioning

confidence: 99%

Lycopene ameliorates neuropathic pain by upregulating spinal astrocytic connexin 43 expression

et al. 2016

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“…Lycopene has recently been shown by immunohistochemical cell staining to specifically increase levels of RARa, and connexin 43 (Cx43) in MCF-7 and MDA-MB-231 breast cancer cell lines, and to strongly increase RARb expression in nontumorigenic MCF10A cells [Chalabi et al, 2007b]. We provide new evidence using methylation specific PCR (MSP) that dietarily relevant, low doses of lycopene (2 lM) can alter DNA methylation of the GSTP1 gene in MDA-MB-468 breast cancer cells, and that this change is coordinated with increased gene expression as suggested by RT-PCR.…”

Section: Introductionmentioning

confidence: 99%

Modulation of gene methylation by genistein or lycopene in breast cancer cells

King-Batoon

Leszczyńska

Klein

2008

Environ and Mol Mutagen

210

113

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Dietary agents with chemopreventive potential, including soy-derived genistein and tomato-derived lycopene, have been shown to alter gene expression in ways that can either promote or potentially inhibit the carcinogenic processes. To begin to explore the mechanisms by which these agents may be acting we have examined the DNA methylation modulating capacity of genistein or lycopene for several genes relevant to breast cancer in the breast cancer cell lines MCF-7 and MDA-MB-468, as well as in immortalized but noncancer fibrocystic MCF10A breast cells. We find using methylation specific PCR (MSP) that a low, nontoxic concentration of genistein (3.125 lM, resupplemented every 48 hr for 1 week) or a single dose of lycopene (2 lM) partially demethylates the promoter of the GSTP1 tumor suppressor gene in MDA-MB-468 cells. RT-PCR studies confirm a lack of GSTP1 expression in untreated MDA-MB-468, with restoration of GSTP1 expression after genistein or lycopene treatment. The RARb2 gene however, was not demethylated by genistein or lycopene in either of these breast cancer cell lines. But, lycopene (2 lM, once per week for 2 weeks) did induce demethylation of RARb2 and the HIN-1 genes in the noncancer MCF10A fibrocystic breast cells. These data show for the first time that the tomato carotenoid lycopene has direct DNA demethylating activity. In summary, both genistein and lycopene, at very low, dietarily relevant concentrations can potentially mitigate tumorigenic processes via promoter methylation modulation of gene expression. Environ. Mol. Mutagen. 49:36-45, 2008.

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Immunohistochemical Expression of RARα, RARβ, and Cx43 in Breast Tumor Cell Lines After Treatment With Lycopene and Correlation With RT-QPCR

Cited by 25 publications

References 21 publications

BRCA1, BRCA2, AR and IGF-I expression in prostate cancer: Correlation between RT-qPCR and immunohistochemical detection

BRCA1, BRCA2, AR and IGF-I expression in prostate cancer: Correlation between RT-qPCR and immunohistochemical detection

Lycopene ameliorates neuropathic pain by upregulating spinal astrocytic connexin 43 expression

Modulation of gene methylation by genistein or lycopene in breast cancer cells

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