Immunohistochemical identification of Toxoplasma gondii in tissues from Modified Agglutination Test positive sheep

Silva, A.F.; Oliveira, Francisco Carlos Rodrigues de; Leite, Juliana da Silva; Mello, Marcela Freire Vallim de; Brandão, F. Z.; Leite, Rosalynn; Frazão-Teixeira, Edwards; Lilenbaum, Walter; Fonseca, Ana Beatriz Monteiro; Ferreira, Ana Maria Reis

doi:10.1016/j.vetpar.2012.09.022

Cited by 24 publications

(24 citation statements)

References 16 publications

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“…The IHC assay performed in the present study was an important tool for the identification of T. gondii in tissues and, consequently, in the confirmation of the disease. The advantage of using IHC was also reported by Motta et al (2008) and Silva et al (2013a), who obtained similar results in sheep tissues. Although toxoplasmosis can be presumptively diagnosed through histopathology, as structures compatible with this protozoan can be visualized (ROSA et al, 2001;LANGONI, 2001), a definitive diagnosis can be obtained using IHC (ANDRADE et al, 2007;CASAGRANDE et al, 2013), which emphasizes the relevance of this test for diagnostic certification.…”

Section: Discussionsupporting

confidence: 80%

Immunohistochemical and serological aspects of Toxoplasma gondii infection in Neotropical primates

Grumann

Silva

Filho

et al. 2017

SCA

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Toxoplasmosis, a disease caused by the intracellular coccidian Toxoplasma gondii that infects most warm-blooded vertebrates, is widely distributed and fatal for primates, which are peculiarly susceptible for unknown reason(s). Owing to the increasing number of Neotropical mammal deaths where in T. gondii were detected in analyzed tissues, the present immunohistochemical study analyzed the distribution patterns of immunostainings related to this parasite on primates necropsied at the Laboratório de Patologia Animal of Universidade de Passo Fundo (UPF), between the years of 2000 and 2014. Furthermore, a serological survey for the disease was conducted for 21 primates from the UPF Zoo, Rio Grande do Sul, Brazil, belonging to genera Sapajus and Alouatta. In a immunohistochemical test performed using streptavidin-biotin-peroxidase, 26.53% positivity was detected in 98 primates. Immunostainings revealed that infection differed among the lung (76.92%), liver (58.33%), heart (50%), brain (42.30%), and kidney (23.07%) tissues. Serology performed through indirect hemagglutination showed reactivity in 85.7% of the animals, all belonging to Sapajus sp., while the three primates that did not show reactivity (14.3%) belonged to Alouatta sp.

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Section: Discussionsupporting

confidence: 80%

Immunohistochemical and serological aspects of Toxoplasma gondii infection in Neotropical primates

Grumann

Silva

Filho

et al. 2017

SCA

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“…This differed from result obtained by Silva (2011), among naturally infected sheep, who reported that the positive staining rate was 52% (12/25), using IHC. Among sheep in Rio de Janeiro, Brazil, Silva et al (2013) concluded that IHC was proven to be effective in making the definitive diagnosis of toxoplasmosis. This was not confirmed in the present study, because the agreement between the PCR and IHC results was moderate (k = 0.44).…”

Section: Discussionmentioning

confidence: 99%

Application of different techniques to detect Toxoplasma gondii in slaughtered sheep for human consumption

Nunes

Silva²,

Oliveira³

et al. 2015

Rev. Bras. Parasitol. Vet.

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The aim of this study was to investigate occurrence of Toxoplasma gondii in sheep slaughtered in the state of Alagoas, Brazil, by means of different diagnosis techniques. Serum samples and tissues from 100 slaughtered sheep were used. To detect antibodies, the indirect immunofluorescence antibody test (IFAT) was used, and tissues from seropositive animals (cut-off ≥1:64) were submitted to Polymerase Chain Reaction (PCR) and immunohistochemistry (IHC). To assess the concordance between the direct techniques, the kappa test was used. In the IFAT, it was observed that 14% (14/100) of the ovine samples were serum-positive. In the PCR, 21.43% (3/14) of the animals were positive and in IHC, it was observed that 7.14% (1/14) were positively stained for T. gondii in cerebral tissue. Histopathologically, the predominant finding was the presence of mononuclear cell infiltrate in the heart and a perivascular cuff in the cerebrum and cerebellum. The concordance between the direct diagnosis techniques was moderate (k=0.44). Thus, it is important to use different direct techniques in diagnosing toxoplasmosis in naturally infected sheep.

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“…Examination by histopathology is widely employed for the diagnosis of T. gondii with only few studies describing the immunohistochemical detection of the protozoan parasite (Pereira-Bueno et al, 2004). However, in a study to determine the association between serology (MAT) and presence of cysts in tissues (IHC) of infected animals, conclusion was drawn that MAT positive animals could serve as potential for human infection because bradyzoites were found in different tissues, regardless of the MAT titration (Silva et al, 2013). Zoonotic visceral leshmaniases for which the domestic dog represents the main reservoir host in urban environments is also one of the important protozoan diseases of humans (WHO, 2010;Chhabra & Singla, 2014 ).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Monoclonal antibodies in immunodiagnostic assays: a review of recent applications

Mahmuda¹,

Bande²,

Abdulhaleem³

et al. 2017

Sokoto J. Vet. Sc.

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Monoclonal antibodies (mAbs) have proven to be effective biological reagents in the immunodiagnostic assays. This is due to their binding accuracy to many pathogens, thus, making them valuable research tools. Since the discovery of hybridoma technology by Kohler and Milstein, the use of monoclonal antibodies produced by the hybrid cells have been employed in diagnosis of several diseases. Monoclonal antibody production has several procedures with considerable variations, but the principles remain the same. Improvements in the field of cell culture technology have led to the production of improved qualities of monoclonal antibodies. In general, these antibodies are important biomedical reagents used in research, especially in the field of laboratory diagnostics for a number of different types of diseases in humans and animals. Some of the areas where application of monoclonal antibodies triumph are herein discussed. This review is aimed to assess various diagnostic assay techniques where monoclonal antibodies are applied in order to provide a first-hand information, especially for beginners in monoclonal antibody production, characterisation, evaluation and /or its applications in research and diagnosis.

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Immunohistochemical identification of Toxoplasma gondii in tissues from Modified Agglutination Test positive sheep

Cited by 24 publications

References 16 publications

Immunohistochemical and serological aspects of Toxoplasma gondii infection in Neotropical primates

Immunohistochemical and serological aspects of Toxoplasma gondii infection in Neotropical primates

Application of different techniques to detect Toxoplasma gondii in slaughtered sheep for human consumption

Monoclonal antibodies in immunodiagnostic assays: a review of recent applications

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