Immunohistochemical localisation of renal cyclooxygenase-1 expression in non-steroidal anti-inflammatory drug-treated mice

Meskell, M; Ettarh, Raj

doi:10.1016/j.etp.2009.09.006

Cited by 4 publications

(4 citation statements)

References 25 publications

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“…In other species, the use of immunohistochemistry to localise COX-isoforms has demonstrated species and age-related differences in expression. Only COX-2 has been localised to the MD in most species, including dogs, rabbits and rats [ 7 , 22 , 23 ], but with both isoforms identified in mice [ 24 ]. Distribution of the isoenzymes in the other compartments of the feline kidney is similar to that seen in rodents, rabbits and dogs [ 7 , 22 , 23 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Cyclooxygenase(COX)-1 and COX-2 inhibition on furosemide-induced renal responses and isoform immunolocalization in the healthy cat kidney

et al. 2015

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BackgroundThe role of cyclooxygenase(COX)-1 and COX-2 in the saluretic and renin-angiotensin responses to loop diuretics in the cat is unknown. We propose in vivo characterisation of isoform roles in a furosemide model by administering non-steroidal anti-inflammatory drugs (NSAIDs) with differing selectivity profiles: robenacoxib (COX-2 selective) and ketoprofen (COX-1 selective).ResultsIn this four period crossover study, we compared the effect of four treatments: placebo, robenacoxib once or twice daily and ketoprofen once daily concomitantly with furosemide in seven healthy cats. For each period, urine and blood samples were collected at baseline and within 48 h of treatment starting. Plasma renin activity (PRA), plasma and urinary aldosterone concentrations, glomerular filtration rate (GFR) and 24 h urinary volumes, electrolytes and eicosanoids (PGE2, 6-keto-PGF1α, TxB2), renal injury biomarker excretions [N-acetyl-beta-D-glucosaminidase (NAG) and Gamma-Glutamyltransferase] were measured. Urine volume (24 h) and urinary sodium, chloride and calcium excretions increased from baseline with all treatments. Plasma creatinine increased with all treatments except placebo, whereas GFR was significantly decreased from baseline only with ketoprofen. PRA increased significantly with placebo and once daily robenacoxib and the increase was significantly higher with placebo compared to ketoprofen (10.5 ± 4.4 vs 4.9 ± 5.0 ng ml−1 h−1). Urinary aldosterone excretion increased with all treatments but this increase was inhibited by 75 % with ketoprofen and 65 % with once daily robenacoxib compared to placebo. Urinary PGE2 excretion decreased with all treatments and excretion was significantly lower with ketoprofen compared to placebo. Urinary TxB2 excretion was significantly increased from baseline only with placebo. NAG increased from baseline with all treatments. Immunohistochemistry on post-mortem renal specimens, obtained from a different group of cats that died naturally of non-renal causes, suggested constitutive COX-1 and COX-2 co-localization in many renal structures including the macula densa (MD).ConclusionsThese data suggest that both COX-1 and COX-2 could generate the signal from the MD to the renin secreting cells in cats exposed to furosemide. Co-localization of COX isoenzymes in MD cells supports the functional data reported here.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-015-0598-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Effect of Cyclooxygenase(COX)-1 and COX-2 inhibition on furosemide-induced renal responses and isoform immunolocalization in the healthy cat kidney

et al. 2015

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“…The number of writhes, consisting of abdominal muscle contractions and hind paw extensions, occurring between 0 and 30 min after acetic acid injection, was recorded. Morphine (5 mg/kg, sc), a non selective opioid agonist, or indomethacin (5 mg/kg, sc), a nonspecific inhibitor of cyclooxygenase [27], were used as controls.…”

Section: Writhing Testmentioning

confidence: 99%

Peripheral antinociception and anti-edematogenic effect of a sulfated polysaccharide from Acanthophora muscoides

Quinderé

Fontes

Vanderlei

et al. 2013

Pharmacological Reports

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“…In control kidneys from mice, COX-1 expression before and after NSAID treatment was investigated (Meskell and Ettarh, 2011). Significant COX-1 expression was noted in the renal cortex and medulla with significantly more expression evident within the cortex.…”

Section: Effects Of Pharmaceutical Inhibition Of Cox-1 and Cox-2 On Cardio-renal Homeostasis Under Normal Physiologic Conditionsmentioning

confidence: 99%

“…Interstitial cells in the renal cortex did not show COX-1 expression. Within the medulla, pronounced COX-1 expression of COX-1 was noted within the endothelial cells of the renal vasculature but the epithelial cells of the medullary collecting ducts and the interstitial cells in the medulla showed poor reactivity for COX-1 (Meskell and Ettarh, 2011;Khan et al, 2013). In the ns-NSAID, indomethacin, treated kidneys, COX-1 expression was present in the cortical and medullary areas.…”

Section: Effects Of Pharmaceutical Inhibition Of Cox-1 and Cox-2 On Cardio-renal Homeostasis Under Normal Physiologic Conditionsmentioning

confidence: 99%

Cardio-renal safety of non-steroidal anti-inflammatory drugs

Radi

Khan

2019

J. Toxicol. Sci.

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Non-steroidal anti-inflammatory drugs (NSAIDs) are among the most widely used therapeutic class in clinical medicine. These are sub-divided based on their selectivity for inhibition of cyclooxygenase (COX) isoforms (COX-1 and COX-2) into: (1) non-selective (ns-NSAIDs), and (2) selective NSAIDs (s-NSAIDs) with preferential inhibition of COX-2 isozyme. The safety and pathophysiology of NSAIDs on the renal and cardiovascular systems have continued to evolve over the years following short-and long-term treatment in both preclinical models and humans. This review summarizes major learnings on cardiac and renal complications associated with pharmaceutical inhibition of COX-1 and COX-2 with focus on preclinical to clinical translatability of cardio-renal data.

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Immunohistochemical localisation of renal cyclooxygenase-1 expression in non-steroidal anti-inflammatory drug-treated mice

Cited by 4 publications

References 25 publications

Effect of Cyclooxygenase(COX)-1 and COX-2 inhibition on furosemide-induced renal responses and isoform immunolocalization in the healthy cat kidney

Effect of Cyclooxygenase(COX)-1 and COX-2 inhibition on furosemide-induced renal responses and isoform immunolocalization in the healthy cat kidney

Peripheral antinociception and anti-edematogenic effect of a sulfated polysaccharide from Acanthophora muscoides

Cardio-renal safety of non-steroidal anti-inflammatory drugs

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