Immunohistochemical localization of a gap junction protein in muscularis externa of murine, canine and human intestine

Mikkelsen, Hanne B.; Huizinga, J D; Thuneberg, Lars; Rumessen, J.J.

doi:10.1016/0016-5085(92)91600-9

Cited by 3 publications

(4 citation statements)

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“…In contrast, Cx 45 immunoreactivity was only detected at gap junctions between ICC-DMP~ICC-DMP. These results are compatible with the observations of Cx 43 immunoreactivities in the outer subdivision of the circular muscle layer of the small intestine in the mouse, dog and human (Mikkelsen et al 1993), and the observation of Cx 45 immunoreactivities in the DMP region of the dogs and rats (Nakamura et al 1998).…”

Section: Discussionsupporting

confidence: 81%

“…The presence of Cx 43 in the intestinal musculature was observed in the mouse, dog and human by immunohistochemical methods (Mikkelsen et al 1993) and in the dog with Western and Northern blotting (Li et al 1993). More recently, Nakamura et al (1998) demonstrated Cx 45 immunohistochemically in the deep muscular plexus of both the dog and rat small intestine, in addition to Cx 43 within the circular muscle layer, and suggested that Cx 45 is localized at gap junctions formed by ICC-DMP in these animals.…”

Section: Introductionmentioning

confidence: 99%

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Immunocytochemical demonstration of the gap junction proteins connexin 43 and connexin 45 in the musculature of the rat small intestine

Seki

Komuro

2001

Cell Tissue Res

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The immunohistochemical localization of connexin (Cx) 43 and Cx 45 in the musculature of the rat small intestine was studied at the ultrastructural level, with special reference to the interstitial cells of Cajal in the deep muscular plexus region (ICC-DMP). Cx 43 was localized at gap junctions formed between every group of cells, i.e., smooth muscle cell~smooth muscle cell, smooth muscle cell--ICC-DMP and ICC-DMP--ICC-DMP. In contrast, Cx 45 immunoreactivity was only detected at gap junctions between ICC-DMP--ICC-DMP. Since different types of Cx molecules have different properties for electrical and chemical coupling of cells, it is suggested that the homotypic network of ICC-DMP connected with Cx 45 gap junctions may function as an independent compartment segregated from the whole cellular network including the smooth muscle cells connected with Cx 43 gap junctions. It is further speculated that the ICC-DMP of the rat small intestine communicate with each other and with smooth muscle cells via the passage of messenger molecules through Cx 43, but they may use an additional mechanism, as yet unknown, for communications restricted to other ICC-DMP.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Immunocytochemical demonstration of the gap junction proteins connexin 43 and connexin 45 in the musculature of the rat small intestine

Seki

Komuro

2001

Cell Tissue Res

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“…In other regions of the human gut, ICC have more prominent myoid features, e.g., prominent filament bundles in association with cytoplasmic dense bodies (ICC in stomach, ICC-MP in small intestine, and ICC in the submuscular plexus), a complete basal lamina (ICC in the deep muscular plexus and in the submuscular plexus), selective synapse-like associations with nerves (ICC in deep muscular plexus in small intestine) or frequent gap junctions to other ICC or muscle cells (ICC in deep muscular plexus). The paucity (or absence) of gap junctions between colonic ICC-MP and other cells is in accordance with our previous studies of connexin-43 reactivity in human colon, a feature shared by ICC at the level of the submuscular plexus (Rumessen et al 1993a(Rumessen et al , 1993bMikkelsen et al 1993).…”

Section: Discussionsupporting

confidence: 88%

Ultrastructure of interstitial cells of Cajal in myenteric plexus of human colon

et al. 2009

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The role of the interstitial cells of Cajal (ICC) associated with the myenteric plexus (ICC-MP) as regulators of the motility of the colonic external muscle remains unclear. Ultrastructural studies of myenteric interstitial cells are lacking in human colon. We therefore characterized the distinctive ultrastructure of these cells in the myenteric region of the colon by transmission electron microscopy of the region between the main muscle layers in all parts of the colon in unaffected areas of resected specimens from nine adult human patients. ICC-MP were similar in various colonic regions and had myoid features such as scattered caveolae, prominent intermediate filaments, and cytoplasmic dense bodies. We found characteristic dense membrane-associated bands with a patchy basal lamina, invaginating cellular protrusions (peg and socket junctions) between ICC and between ICC and muscle cells, and close contacts (<100 nm) between ICC and nerves. No gap junctions were observed. Fibroblast-like cells (FLC) were abundant showing well-developed secretory organelles, including coated vesicles, but lacked prominent intermediate filaments and caveolae. FLC had a patchy basal lamina, and peg and socket junctions were observed between them. Macrophage-like cells frequently occurred in close apposition with FLC and, more seldomly, with ICC-MP. The ultrastructure of ICC and FLC in the myenteric region of the human colon thus differs characteristically, but significant overlaps in the ultrastructure between ICC and FLC might complicate any interpretation in pathological ultrastructural studies of the human colonic muscle layer.

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“…The objective of this study was to use immunocytochemistry to evaluate whether any gap junctions of canine gastrointestinal tissues are composed exclusively of Cx43, as is sometimes implied (24,26), or whether they contain other connexins such as Cx45, which has recently been reported in canine DMP (28), or Cx40, which has been reported in other smooth muscles (25). Although Cx37 has recently been reported to be present in airway smooth muscle (27) and sparsely present in vascular smooth muscle (39), it was not studied.…”

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confidence: 99%

Gap junctions in gastrointestinal muscle contain multiple connexins

Wang

Daniel

2001

American Journal of Physiology-Gastrointestinal and Liver Physi

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In the canine gastrointestinal tract, the roles that gap junctions play in pacemaking and neurotransmission are unclear. Using antibodies to connexin (Cx)43, Cx45, and Cx40, we determined the distribution of these connexins. Cx43 was present in all locations where structural gap junctions occur. Cx40 was also widely distributed in the circular muscle of the lower esophageal sphincter (LES), stomach, and ileum. Cx45 was sparsely distributed in circular muscle of the LES. In the interstitial cells of Cajal (ICC) networks of myenteric plexus, in the deep muscular and submuscular plexuses, sparse Cx45 and Cx40 immunoreactivity was present. In colon, immunoreactivity was found only in the myenteric and submuscular plexus and nearby circular muscle cells. No immunoreactivity was found in sites lacking structural gap junctions (longitudinal muscle, inner circular muscle of the intestine, and most circular muscle of the colon). Studies of colocalization of connexins suggested that in the ICC networks, some colocalization of Cx43 with Cx40 and/or Cx45 occurred. Thus gap junctions in canine intestine may be heterotypic or heteromeric and have different conductance properties in different regions based on different connexin compositions.

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Immunohistochemical localization of a gap junction protein in muscularis externa of murine, canine and human intestine

Cited by 3 publications

References 0 publications

Immunocytochemical demonstration of the gap junction proteins connexin 43 and connexin 45 in the musculature of the rat small intestine

Immunocytochemical demonstration of the gap junction proteins connexin 43 and connexin 45 in the musculature of the rat small intestine

Ultrastructure of interstitial cells of Cajal in myenteric plexus of human colon

Gap junctions in gastrointestinal muscle contain multiple connexins

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