Immunohistochemical localization of endocrine cells in the alimentary tracts of six frog species

Immunohistochemistry of endocrine cells in the alimentary tract of the tree frog,hyla arborea japonica

“…In the present study, these cells were observed in the whole alimentary tract as in previous reports but relative frequencies are somewhat different from other anuran species and the relative frequencies in the intestinal tract were quite ÜHHHH^^^^^H^^^^BH . different from reported reparted by Choi et al (1999).…”

“…The regional distributions and relative frequencies of serotoninimmunoreactive cells were detected in the whole GIT of Rana dybowskii (Lee and Lee, 1996), Xenopus laevis (Lee and Lee, 1992), Rana nigromaculata (Lee et al, 1998b) and Rana catesbeiana (Lee et al, 1999). In addition, Choi et al (1999) reported the localization of serotonin-immunoreactive cells in the alimentary tracts of six frog species habited in Korea including the tree frog. According to these previous reports, these cells were most predominant in the pylorus except for Rana nigromaculata (Lee et al, 1998b) which were most predominant in the fundus and duodenum.…”

“…It is well known that somatostatin-immunoreactive cells show the widest distribution in the whole GIT of all vertebrate species investigated, including the primitive agnathans with serotonin-immunoreactive cells (Falkmer and Van Noorden, 1983). In the anuran species, these cells were detected in Rana dybowskii (Lee and Lee, 1996), Rana esculenta (Trandaburu and Nürnberger, 1995), Xenopus laevis (Lee and Lee, 1992), Bufo regularis (El-Sahly et al, 1981), Rana nigromaculata (Lee et al, 1998b), Rana catesbeiana (Lee et al, 1999), 8 species of Anura (Buchan, 1986) and six frog species which inhabit Korea including the tree frog (Choi et al, 1999). According to these reports, they were most predominant in the fundus but decreased distally along the GIT except for the tree frog which were most predominant in the pylorus and thereafter decreased toward the distal portion of the intestine and finally was not detected in the rectum (Choi et al, 1999).…”

“…In the anuran species, these cells were detected in Rana dybowskii (Lee and Lee, 1996), Rana esculenta (Trandaburu and Nürnberger, 1995), Xenopus laevis (Lee and Lee, 1992), Bufo regularis (El-Sahly et al, 1981), Rana nigromaculata (Lee et al, 1998b), Rana catesbeiana (Lee et al, 1999), 8 species of Anura (Buchan, 1986) and six frog species which inhabit Korea including the tree frog (Choi et al, 1999). According to these reports, they were most predominant in the fundus but decreased distally along the GIT except for the tree frog which were most predominant in the pylorus and thereafter decreased toward the distal portion of the intestine and finally was not detected in the rectum (Choi et al, 1999). In the present study, somatostatin-immunoreactive cells were detected throughout the whole alimentary tract except for the rectum and the regional distributions and relative frequencies agreed with those reported by Choi et al (1999).…”

“…The GEP endocrine cells in various amphibians have been extensively studied by histochemical (Kim and Chung, 1973), electron microscopical (Gauze, 1971;Chung and Kwun, 1983) and immunohistochemical (Buchan, 1986) methods. Although about 17 types of endocrine cells including serotonin, somatostatin, glucagon, cholecystokinin (CCK)-8, chromogranin, pancreatic polypeptide (PP), bombesin, neurotensin, gastrin-releasing peptide, substance P, polypeptide YY, secretin, gastrin, vasoactive intestinal polypeptide (VIP), motilin, met-enkephalin, ß-enkephalin and others have been detected in Rana dybowskii (Lee and Lee, 1996), Rana pipens (Lechago et al, 1978), Xenopus laevis (Lechago et al, 1978;Lee, 1992, 1997), Rana esculenta (Trandaburu and Nürnberger, 1995), Bufo regulahs (El-Salhy et al, 1981, 1982, Rana catesbeiana (Lee et al, 1998a(Lee et al, , 1999, Rana nigromaculata (Lee et al, 1998b) and 8 species of the anuran amphibian (Buchan, 1986), the endocrine cells of the tree frog have not been extensively studied except by Choi et al (1999) who reported the localization of endocrine cells in the alimentary tracts of six frog species which inhabited Korea including the tree frog, Hyla arbórea japónica. Their studies did not show any sufficient evidence namely, they used only six typed antisera (somatostatin, gastrin, CCK-8, PP, serotonin and glucagon).…”