Immunohistochemical Localization of Epidermal Growth Factor and Its Effect on Granulosa Cell Proliferation in Rat Ovary.

Fukumatsu, Y.; Katabuchi, Hidetaka; Okamura, Hitoshi

doi:10.1507/endocrj.42.467

Cited by 13 publications

(9 citation statements)

References 27 publications

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“…Cytokines produced by immune cells such as macrophages are involved in various physiological events, such as follicular development, atresia, ovulation, and luteolysis in ovaries [33,34]. Macrophages are present in the ovaries of many animal species, but it has not been clear whether they are located in the theca layer, granulosa layer, or follicular antrum [35][36][37]. In the present study, we clearly demonstrated that macrophages are located only in theca external and internal layers of healthy follicles, and that they are not in the granulosa layer or follicular antrum.…”

Section: Discussionmentioning

confidence: 99%

The Role of Interleukin-6 in the Regulation of Granulosa Cell Apoptosis During Follicular Atresia in Pig Ovaries

Maeda

Inoue

Matsuda‐Minehata

et al. 2007

Journal of Reproduction and Development

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Abstract. More than 99% of follicles in mammalian ovaries undergo a degenerative process known as atresia, and only a few follicles actually ovulate during follicular growth and development. Follicular selection mostly depends on granulosa cell apoptosis, but the molecular mechanism behind the regulation of this selective atresia is still largely unknown. In the present study, to examine whether or not interleukin-6 (IL-6), a multifunctional cytokine, is involved in apoptosis during atresia in pig ovaries, the expression of IL-6 mRNA in granulosa cells was quantified by real-time reverse transcription-polymerase chain reaction (RT-PCR). The level of mRNA decreased during atresia. Enzyme-linked immunosorbent assay (ELISA) showed that the level of IL-6 protein in follicular fluid also decreased during atresia. Moreover, recombinant human IL-6 upregulated the expression of an intracellular apoptosis inhibitor, cellular FLICE-like inhibitory protein long form (cFLIPL), in cultured cells derived from human granulosa cells. It is possible that IL-6 is produced in the granulosa cells of healthy follicles, that it increases the cFLIPL level, and cFLIPL then prevents apoptotic cell death.

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Section: Discussionmentioning

confidence: 99%

The Role of Interleukin-6 in the Regulation of Granulosa Cell Apoptosis During Follicular Atresia in Pig Ovaries

Maeda

Inoue

Matsuda‐Minehata

et al. 2007

Journal of Reproduction and Development

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“…However, recent reports have demonstrated the appearance of macrophages within the granulosa layer and antrum of ovarian growing follicles without any signs of atresia (LoUKIDES et al, 1990;FUKUMATSU et al, 1992FUKUMATSU et al, , 1995BUKOVSKI et al, 1993BUKOVSKI et al, , 1995. It seems thus safe to suppose that macrophages enter the follicles before follicular maturation.…”

Section: Discussionmentioning

confidence: 99%

“…The present finding that phagocytic granulosa cells act as scavengers even without the assistance by macrophages in some mature follicles may indicate that macrophages do not play a leading role in the elimination of apoptotic granulosa cells. On the other hand, previous investigators suggested that macrophages might stimulate the growth of granulosa cells with some growth factors in a paracrine fashion (FUKUMATSU et al, 1992(FUKUMATSU et al, , 1995BUKOVSK? et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Elimination of Apoptotic Granulosa Cells by Intact Granulosa Cells and Macrophages in Atretic Mature Follicles of the Guinea Pig Ovary.

Kasuya

1997

Arch. Histol. Cytol.

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“…Recent studies have documented roles for EGF in the ovarian physiology. Indeed, EGF, its receptor, and mRNA were detected in the hamster, rat, and human granulosa and theca cells [4][5][6]. EGF was also detected in human and porcine follicular fluid [7,8].…”

mentioning

confidence: 98%

In Vivo Effect of Epidermal Growth Factor, Interleukin-1β, and Interleukin-1RA on Equine Preovulatory Follicles1

Martoriati

Duchamp

Gérard

2003

Biology of Reproduction

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Paracrine factors have significant effects during folliculogenesis. Because of various morphological features, the mare is a convenient model to study in vivo the effects of factors involved in periovulatory events. In the present work, epidermal growth factor (EGF; experiment 1, n = 49 mares) and interleukin-1beta and interleukin-1RA (IL-1beta and IL-1RA, respectively; experiment 2, n = 80 mares) were injected intrafollicularly to evaluate the influence of these factors on in vivo maturation of equine preovulatory follicles. A transvaginal ultrasound-guided injection was performed when the diameter of the dominant follicle reached 30-34 mm. In experiment 1, the four experimental groups were 1) EGF group, intrafollicular (i.f.) injection of EGF (2 ml; 0.5 microg/ml) plus i.v. injection of physiological serum; 2) control group, no injection; 3) PBS group, i.f. injection of 2 ml of PBS plus i.v. injection of physiological serum; 4) crude equine gonadotropins (CEG) group, i.f. injection of PBS plus i.v. injection of CEG (20 mg). In experiment 2, groups 3 and 4 were the same as in experiment 1, but groups 1 and 2 were changed as follows: 1) IL-1beta group, i.f. injection of IL-1beta (2 ml; 0.5 microg/ml) plus i.v. injection of physiological serum; 2) IL-1RA group, i.f. injection of IL-1RA (2 ml; 0.5 microg/ml) plus i.v. injection of physiological serum. In each experiment, cumulus-oocyte complexes from dominant/injected follicles were collected by transvaginal ultrasound-guided aspiration 38 h after intrafollicular injection. Cumulus morphology and oocyte nuclear stage were assessed. Additionally, in experiment 2, 40 mares were used to determine the time of ovulation after treatments. Our results indicate that intrafollicular injection of EGF or PBS induced lower cumulus expansion and oocyte maturation rates compared with the CEG group (P < 0.05). In experiment 2, the IL-1beta and CEG groups showed the same expansion rate, the same oocyte maturation rate, and the same ovulation distribution. On the other hand, the intrafollicular injection of IL-1RA, as PBS, did not induce follicle and cumulus-oocyte complex (COC) maturation. In conclusion, we confirmed that the technique of intrafollicular injection can be used in the mare to study the role of specific molecules. We demonstrated for the first time in mares that the injection of EGF did not influence in vivo COC maturation. In contrast, IL-1beta injection into the dominant follicle induced in vivo oocyte maturation and the ovulation process whereas IL-1RA seemed to block these mechanisms.

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Immunohistochemical Localization of Epidermal Growth Factor and Its Effect on Granulosa Cell Proliferation in Rat Ovary.

Cited by 13 publications

References 27 publications

The Role of Interleukin-6 in the Regulation of Granulosa Cell Apoptosis During Follicular Atresia in Pig Ovaries

The Role of Interleukin-6 in the Regulation of Granulosa Cell Apoptosis During Follicular Atresia in Pig Ovaries

Elimination of Apoptotic Granulosa Cells by Intact Granulosa Cells and Macrophages in Atretic Mature Follicles of the Guinea Pig Ovary.

In Vivo Effect of Epidermal Growth Factor, Interleukin-1β, and Interleukin-1RA on Equine Preovulatory Follicles1

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