Immunohistochemical localization of laminin within the mouse ovary

Wordinger, Robert J.; Rudick, Victoria L.; Rudick, Michael J.

doi:10.1002/jez.1402280116

Cited by 18 publications

(12 citation statements)

References 5 publications

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“…By immunohistochemistry using antibodies raised against LN-1, we have confirmed the presence of LN in basement membrane, granulosa and theca cells of ovine antral follicles, as shown previously in various animal species (Bagavandoss et al 1983, Wordinger et al 1983, Bortolussi et al 1989, Leardkamolkarn & Abrahamson 1992, Zhao & Luck 1995, Lee et al 1996, Huet et al 1997, van Wezel et al 1998, Alexopoulos et al 2000. Laminins are heterotrimeric proteins containing , and subunits, and the association of structural isoforms of these subunits gives rise to different LN (Mercurio 1995).…”

Section: Discussionsupporting

confidence: 85%

“…This basement membrane is believed to play a role in influencing proliferation and differentiation of GC (Amsterdam et al 1989, Luck 1994 and in protecting them from entering apoptosis in early atretic follicles (Monniaux et al 1999). Laminin (LN) has previously been shown to be one of the most abundant ECM components within the basement membrane (Wordinger et al 1983, Leardkamolkarn & Abrahamson 1992, Zhao & Luck 1995, Lee et al 1996, Huet et al 1997, van Wezel et al 1998, Alexopoulos et al 2000. In vitro, purified LN used as a culture substratum improves GC survival in rat (Aharoni et al 1996) and sheep (Huet et al 2001) and stimulates their proliferation in sheep (Huet et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Bellego¹,

Pisselet²,

Huet³

et al. 2002

Journal of Endocrinology

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This study aimed to determine the physiological role of laminin (LN) and its receptor, 6 1 integrin, in controlling the functions of granulosa cells (GC) during follicular development in sheep ovary. Immunohistochemistry experiments showed the presence of increasing levels of LN (P<0·0001), and high levels of mature 6 1 integrin in GC layers of healthy antral follicles during the follicular and the preovulatory phases of the estrous cycle. In vitro, the addition of a function-blocking antibody raised against 6 subunit (anti-6 IgG) to the medium of ovine GC cultured on LN impaired cell spreading (P<0·0001), decreased the proliferation rate (P<0·05) and increased the apoptosis rate (P<0·05). Furthermore, addition of anti-6 IgG enhanced estradiol (E2) secretion by GC in the presence or absence of follicle-stimulating hormone (FSH), luteinizing hormone or insulin-like growth factor-I in culture medium (P<0·0001), and inhibited progesterone (P4) secretion in basal conditions or in the presence of low (0·5 ng/ml) FSH concentrations only (P<0·0001). The anti-6 IgG effect was specific to an interaction of LN with 6 1 integrin since it was ineffective on GC cultured on heat-denatured LN, RGD (arginine-glycine-aspartic acid) peptides and non-coated substratum. Hence, this study established that 6 1 integrin 1) was expressed in GC of antral follicles, 2) mediated the actions of LN on survival, proliferation and steroidogenesis of GC, and 3) was able to dramatically modulate P4 and E2 secretion by GC in vitro. It is suggested that during the follicular and the preovulatory phases of the estrous cycle, the increasing levels of LN in GC of large antral follicles might support their final development to ovulation.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Bellego¹,

Pisselet²,

Huet³

et al. 2002

Journal of Endocrinology

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“…Collagen type IV, laminin, and heparan sulfate proteoglycans are present in the basement membrane of the rodent ovarian follicles [19][20][21]. Interestingly, immunoelectron microscopic studies of rat ovaries have shown that laminin is absent from the primordial follicles but is detected in the preantral and antral follicles [22].…”

Section: Discussionmentioning

confidence: 94%

Interaction of Extracellular Matrix and Activin-A in the Initiation of Follicle Growth in the Mouse Ovary1

Oktay

Karlikaya

Akman

et al. 2000

Biology of Reproduction

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The precise mechanism for the initiation of follicle growth and progression through the earliest stages of follicle development remains largely unknown. Activins play a role during early follicle development, and evidence suggests that the extracellular matrix plays a role during later stages of follicular growth. We investigated the role of activin-A and extracellular matrix in follicle growth initiation and early follicular development in the mouse ovary. Ovaries were collected from 5-day-old mice and cultured for 10 days on polylysine, collagen, or laminin in the presence or absence of recombinant human activin-A. Follicle density, indices of follicle growth initiation (primary:primordial follicle [PY:PD] and primary:total follicle [PY:TF] ratios), ratios of multilayer follicle:total follicle (ML:TF), and follicle growth rates were compared between groups. Follicle densities were significantly higher in the extracellular matrix treatment group compared with the polylysine group (P < 0.01). Also, compared with polylysine, both collagen and laminin significantly increased indices of follicle growth initiation (PY:PD ratio: P < 0.001, odds ratio of 3.3; PY:TF ratio: P < 0.001, odds ratio of 2.5), and these were not altered by activin treatment. In the absence of activin-A, exposure to neither collagen nor laminin had an effect on multilayer follicle development. When activin-A was added, collagen and laminin had opposing effects on multilayer follicle development. Activin-A stimulated multilayer follicle development in the presence of laminin (ML:TF ratio: P = 0.01, odds ratio of 10.8), whereas it suppressed follicle growth in collagen (P = 0.01). Activin-A did not affect the ML:TF ratio in the polylysine-treated groups. These results strongly suggest that extracellular matrix components and activin-A interact with each other, and that they regulate follicle growth initiation and multilayer follicle development.

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“…Unfortunately, the composition, synthesis, regulation, and function of the follicular basal lamina that surrounds the membrana granulosa from primordial to preovulatory stages are poorly understood. Type IV collagen, fibronectin, and laminin have been immunolocalized to the follicular basal lamina [44][45][46][47], and our recent evidence suggests that bovine granulosa cells synthesize at least the structural components of the follicular basal lamina [31,32]. Although some components of the basal lamina in some organs are secreted by the stroma [48,49], in most cases the structural components are secreted by epithelial cells (see [50]).…”

Section: Figmentioning

confidence: 99%

Morphological Characterization of Bovine Primordial Follicles and their Environment in Vivo1

Wezel

Rodgers

1996

Biology of Reproduction

140

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Classically, primordial follicles have flattened pre-granulosa cells that become cuboidal and divide during follicular activation. This change in shape is thus an index of activation. To examine this and follicular morphology in cattle, ovaries were processed for light and electron microscopy (n = 21). In single sections (5 microns thick), primordial follicles had a mean ratio of maximum to minimum diameters of 1.33 +/- 0.18 (mean +/- SD; n = 317, randomly selected), indicating that they had a prolate rather than a spherical shape. The prolate shape was attributable to clustering of granulosa cells at two opposite poles on the long axis of each follicle; and in each histological section, 82.5% of primordial follicles (n = 317) had at least one cuboidal granulosa cell. More accurate measurements of primordial follicles indicated that they had three dimensions measured as length (45.4 +/- 2.4 microns), breadth (26.8 +/- 1.5 microns), and depth (< 30.4 +/- 1.4 microns) (mean +/- SEM; n = 12, constructed from serial sections); a surface area of 0.0032 mm2; and 24 +/- 2 granulosa cells. The ultrastructural morphology of the cells of the bovine primordial follicle (n = 31 examined by electron microscopy) was similar to that of other species. The ovarian cortex was composed of discrete zones. The zones containing primordial follicles were substantially avascular, as observed by localizing von Willebrand factor, and rich in collagen fibrils. In conclusion, since primordial follicles of bovine ovaries contain some cuboidal pre-granulosa cells, changes in shape cannot be used with certainty as a marker of activation of bovine follicles.

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Immunohistochemical localization of laminin within the mouse ovary

Cited by 18 publications

References 5 publications

Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Interaction of Extracellular Matrix and Activin-A in the Initiation of Follicle Growth in the Mouse Ovary1

Morphological Characterization of Bovine Primordial Follicles and their Environment in Vivo1

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