Immunohistochemical Localization of Neuropeptides in Bovine Pancreas

Abstract

The occurrence and density of distribution of nerves and endocrine cells that are immunoreactive for neuropeptides in the bovine pancreas were studied by immunohistochemistry. The six neuropeptides localized were galanin (GAL), substance P (SP), methionine-enkephalin (MENK), neuropeptide Y (NPY), calcitonin gene-related peptide (CGRP) and vasoactive intestinal polypeptide (VIP). The exocrine pancreas was shown to have an appreciable number of GAL- and SP-immunoreactive nerve fibres but few fibres showing immunoreactivity for VIP and CGRP. Numerous MENK-, GAL-, SP-, and NPY-immunoreactive nerve fibres were seen in the endocrine portion of the pancreas. Nerve cell bodies in the intrapancreatic ganglia showed immunoreactivity for all of the neuropeptides except CGRP. Endocrine cells showing immunoreactivity for GAL and SP were observed in the large islets and islets of Langerhans, respectively. The present results indicate a characteristic distribution of neuropeptides in the bovine pancreas, which may regulate both exocrine and endocrine secretions of pancreas.

Galanin-like Immunoreactive Neural Elements in Domestic Ruminant Pancreas.

“…It also confirmed previous findings on its presence not only in the neural elements but also in the large islet endocrine cells of cattle [2,17]. The Gal-IR endocrine cells in the large islets may be a peculiar morphological feature of the large but not the small ruminant species.…”

“…This physiological action of galanin coupled with its effect on pancreatic exocrine secretion [18,23] and release of other pancreatic hormones such as pancreatic polypeptide [3,20], glucagon [3,5,9,11,12,16] and somatostatin [3,5,9,16] [1,14,19]. Recently, we have reported striking findings on the presence of galanin-like immunoreactivity not only in the neural elements but also in the endocrine cells of the large islets of cattle, a large ruminant species [2,17]. Large islet is one of the two distinct types of pancreatic islets in cattle [4].…”

“…Recently, we have reported striking findings on the presence of galanin-like immunoreactivity not only in the neural elements but also in the endocrine cells of the large islets of cattle, a large ruminant species [2,17]. Large islet is one of the two distinct types of pancreatic islets in cattle [4].…”

Immunodetection of cocaine- and amphetamine-regulated transcript in bovine pancreas

“…Such peptides include the P (SP) substance and the Y (NPY) neuropeptide, vasoactive intestinal peptide (VIP) Myojin et al, 2000), pituitary adenylate cyclase-activating polypeptide (PACAP) (Fridolf et al, 1992), calcitonin gene-related peptide (CGRP) (Fujimura et al, 1988;Myojin et al, 2000;Sternini et al, 1992), galanin (Gal) (Adeghate and Ponery, 2001;Baltazar et al, 2000;Myojin et al, 2000) and somatostatin (ST) (Fujimura et al, 1988).…”

“…Nerves with CART structures have been found in the bovine abomasum . In polygastric animals, studies have been carried out to investigate the localization and distribution of such peptides as Gal, CGRP or NPY Myojin et al, 2000). In turn, Arciszewski and his team analyzed the expression of neuronal nitric oxide synthase (NOS) and co-localization of selected peptides in nerve structures of the ovine pancreas (Arciszewski and Zacharko-Siembida, 2007;Arciszewski, 2007).…”