Immunohistochemical localization of the InsP4 receptor GTPase-activating protein GAP1IP4BP in the rat brain

Signore, Armando P.; O'Rourke, F; Lu, Xinghua; Feinstein, Maurice B.; Yeh, Hermes H.

doi:10.1002/(sici)1097-4547(19990201)55:3<321::aid-jnr7>3.0.co;2-a

Cited by 3 publications

(1 citation statement)

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“…Immunohistochemical analysis revealed high protein levels in the CA1 region of the hippocampus, but low protein expression in CA3 and dentate gyrus. At the subcellular level, Rasa3 is found in cell bodies (endomembranes and plasma membrane, less in the cytosol) and proximal apical dendrites (Signore et al, 1999).…”

Section: Ii53 Roles Of Ras Gaps In the Brainmentioning

confidence: 99%

The Role of the Ras Guanyl-Nucleotide Exchange Factor Rasgrp1 in Synaptic Transmission

Bungers¹

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Symbol Name Other designations, notes Calb1 Calbindin D-28K, calbindin D28 Calb2 Calretinin, Calbindin2 CR Camk2a calcium/calmodulin-dependent protein kinase II alpha CamkII subunit alpha Canx Calnexin Cnx Creb1 cAMP responsive element binding protein 1 Dab2ip disabled homolog 2 (Drosophila) interacting protein Dgkz diacylglycerol kinase zeta Diap1 diaphanous homolog 1 Dia1 Dlg2 disks large homolog 2 PSD-93, Psd93, Chapsyn-110 Dlg4 disks large homolog 4 PSD-95, Psd95 Eea1 early endosome antigen 1 Egf Epidermal growth factor Gad1 glutamic acid decarboxylase 1 EP10, GAD25, GAD44, GAD67 Gad2 glutamic acid decarboxylase 2 GAD65 Golgb1 Giantin, golgi autoantigen, golgin subfamily b, macrogolgin 1 Gm6840 Grb2 growth factor receptor-bound protein 2 Gria1 glutamate receptor, ionotropic, AMPA1 (alpha 1) GluR1, GluA1, GluRA Gria2 glutamate receptor, ionotropic, AMPA2 (alpha 2) GluR2, GluA2, GluR-B, Glur-Diese Studie enthält die ersten Beweise für eine spezifische neuronale Funktion von Rasgrp1. Sie zeigt, dass Rasgrp1 selektiv die postsynaptische Sensitivität an glutamatergen Synapsen reguliert. Diese Studie zeigt, dass die selektive Veränderung der Regulation von Ras eine hilfreiche Methode ist, um die vielfältigen Effekte der Ras Signaltransduktion in Neuronen verstehen zu können.represent the postsynapse. Upon binding of Glutamate, the receptors open and allow Sodium to enter the cell. Upon this influx of cations, the cell depolarizes locally. This electrical signal propagates as the depolarization passively spreads through the dendrites. Spine shape, dendrite caliber and branching influence signal propagation as it travels to the soma. In the soma, final integration of all incoming signals takes place. If the resulting depolarization passes a certain threshold, a new action potential is generated in an all-or-none fashion.Postsynaptic spines, dendrites, the soma, the axon and presynaptic boutons represent highly specialized compartments that are part of the complex morphology of neurons. In fact, without knowledge of ion channels or biophysical properties of the II.3. Controversies in the Research of Neuronal Ras SignalingSince publication of the findings described above, research on neuronal Ras signaling has led to many controversies. In this regard, one experimental system in particular appears to have given rise to most of the controversies in the field.Extensive research on Ras signaling is conducted using activated mutants of Ras proteins. The activated mutated protein is able to bind GTP, but unable to hydrolyze it to GDP and therefore remains in a constitutively active state (Karnoub and Weinberg, 2008). The corresponding frequently used Glycine to Valine mutation at amino acid (aa) position 12 (G12V) is normally found in oncogenic Hras.The first electrophysiological study linking Ras signaling to LTP was in fact a KO study on Rasgrf1 (also known as cdc25NEF, RasGRF, GRF1), a Ras activator.The protein had been identified and cloned by three independent groups (Cen et al., 1992;Martegani et al., 1992;Shou et al....

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Section: Ii53 Roles Of Ras Gaps In the Brainmentioning

confidence: 99%

The Role of the Ras Guanyl-Nucleotide Exchange Factor Rasgrp1 in Synaptic Transmission

Bungers¹

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Cellular expression and subcellular localization of the human Ins(1,3,4,5)P4-binding protein, p42IP4, in human brain and in neuronal cells

Sedehizade

Hanck

Stricker

et al. 2002

Molecular Brain Research

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GAP1(IP4BP)/RASA3 Mediates Gαi-induced Inhibition of Mitogen-activated Protein Kinase

Nafisi¹,

Banihashemi

Daigle

et al. 2008

Journal of Biological Chemistry

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The dopamine D2S receptor (short isoform) couples to inhibitory G␣ i/o proteins to inhibit thyrotropin-releasing hormone (TRH)-stimulated p42/p44 mitogen-activated protein kinase (ERK1/2) phosphorylation in GH4ZR7 rat pituitary cells, consistent with its actions to inhibit prolactin gene transcription and cell proliferation. However, the underlying mechanism is unclear. To identify novel G␣ i effectors, yeast two-hybrid screening of a GH4ZR7 cDNA library was done using constitutively active G␣ i3 -Q204L, and multiple clones of the RasGAP cDNA GAP1 Five dopamine receptor genes are known, and the dopamine-D2 receptor is among the most studied because of its involvement in mental and neurological disorders such as schizophrenia, addiction, and Parkinson disease. In addition, the dopamine-D2 receptor mediates inhibitory regulation of endocrine function, with a primary role in the pituitary to regulate prolactin synthesis, secretion, and lactotroph cell proliferation. For example, in homozygous mice deficient in the gene encoding the D2 receptor or lacking dopamine, pituitary adenoma and hyperprolactinemia occur with age (1-4). Conversely, mice lacking the gene encoding the dopamine transporter show dopamine hypersecretion that leads to pituitary hypotrophy (5). In addition, dopamine-D2 receptor agonists such as bromocryptine or cabergoline are used clinically to induce regression of pituitary adenomas (6 -9). The dopamine D2 receptor gene contains an alternately spliced exon encoding 29 amino acids in the putative third intracellular loop to generate short (D2S) and long (D2L) forms of the receptor (10). Dopamine D2S receptors have been shown to have anti-proliferative actions in pituitary lactotrophs, whereas the D2L receptor did not appear to be anti-proliferative (11). However, the specific signaling mechanisms underlying dopamine D2S actions in pituitary cells remain to be fully elucidated.GH4ZR7 cells are GH4C1 lactotroph cells stably transfected with the dopamine D2S receptor cDNA. These cells provide a useful model to study the signaling of the dopamine-D2S receptor in pituitary cells that retain differentiated properties of lactotrophs. These include expression of receptors that stimulate (thyrotropin-releasing hormone (TRH) 3 receptors) or inhibit (somatostatin and muscarinic receptors) the synthesis and secretion of growth hormone and prolactin (PRL) (12). In GH4ZR7 cells, dopamine inhibits cAMP formation, PRL synthesis and secretion, and cell proliferation. Previously, a novel D2S receptor-mediated inhibition of TRH-induced ERK1/2 activation was identified in these cells (13,14). Interestingly, D2L receptors did not couple to this pathway (15). Activation of ERK1/2 is implicated in TRH induced prolactin transcription (16), and its inhibition is a key pathway for dopamine-D2S-induced inhibition of prolactin transcription (17). This pathway is also observed in primary striatal cultures, suggesting a general role for this D2S receptor-mediated signaling pathway in neuroendocrine tissues (15).

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Immunohistochemical localization of the InsP4 receptor GTPase-activating protein GAP1IP4BP in the rat brain

Cited by 3 publications

References 46 publications

The Role of the Ras Guanyl-Nucleotide Exchange Factor Rasgrp1 in Synaptic Transmission

The Role of the Ras Guanyl-Nucleotide Exchange Factor Rasgrp1 in Synaptic Transmission

Cellular expression and subcellular localization of the human Ins(1,3,4,5)P4-binding protein, p42IP4, in human brain and in neuronal cells

GAP1(IP4BP)/RASA3 Mediates Gαi-induced Inhibition of Mitogen-activated Protein Kinase

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