Immunohistochemical Markers in the Assessment of Tumor Response

Abstract

The examination of the possibility of using immunohistochemical and molecular genetic markers as predictors of effectiveness of neoadjuvant chemoradiotherapy (NCRT) and prognostic factors of the disease state. The study included 21 patients with locally advanced gastric cancer. All patients underwent the NCRT followed by gastrectomy D2. We analyzed the expression of HER2 / neu marker, Ki-67, p53, Cyklin D1, E-cadherin in biopsy (before therapy) and the operating material (after chemoradiotherapy and the treatment gap). We have found statistically significant decrease in the expression of Ki-67 markers and Cyklin D1, a trend towards to a decrease of p53 expression after the NCRT. The dynamics of expression of immunohistochemical markers examination is a promising approach in search for predictors of NCRT effectiveness for patients with locally advanced gastric cancer.

Photodynamic action of photosensitizers based on polycationic derivatives of synthetic bacteriochlorin against human lung cancer cells A549

“…After that, the slides were washed in phosphate buffer, dried, fixed in ice-cold 96% methanol for 10 min and re-dried. An immunohistochemical study was carried according to standard protocols [8,9]. Monoclonal antibodies to Caspase-3 (Cas3) and Ki-67 (readytouse, NOVOCASTRA, Great Britain) were used as primary antibodies.…”