Immunohistochemical, molecular, and cytogenetic analysis of a consecutive series of 20 peripheral t‐cell lymphomas and lymphomas of uncertain lineage, including 12 Ki‐I positive lymphomas

Abstract

Although several independent series of non-Hodgkin's lymphomas (NHLs) have been subjected to cytogenetic studies or analyses of lineages by assaying for clonal immunophenotypes and clonal rearrangements affecting immunoglobulin (IG) and T-cell receptor (TCR) genes, no published reports exist of series of non-B-cell NHLs on which cytogenetic, immunohistochemical, and IG and TCR gene rearrangement studies have been undertaken together. Among 343 NHLs ascertained prospectively between January 1984 and December 1988 at the Memorial Sloan-Kettering Cancer Center, 278 cases with clonal chromosome abnormalities were identified. Of the latter, 20 were non-B-cell NHLs, which in turn comprised 15 peripheral T-cell lymphomas (PTCLs) and 5 lymphomas of uncertain lineage (LULs). The LULs either were biogenotypic, had discordant immunophenotype and immunogenotype, or showed no evidence of B-cell, T-cell, or histiocytic derivation. Of the 15 PTCLs, eight expressed the Ki-1 antigen and four of these had translocations involving the band 5q35 [t(5q35)]. Of the five LULs, four expressed the Ki-1 antigen and one of these had a translocation involving band 5q35. Previous studies have associated t(5q35) with Ki-1 positive NHLs characterized histologically by a pleomorphic diffuse large cell morphology. In our series of 12 Ki-positive non-B-cell NHLs, five (42%) had a 5q35 translocation. They were histologically indistinguishable from the subset without the translocation. The frequent lineage uncertainty exhibited by Ki-1 positive NHLs of similar histology and cytogenetic abnormalities suggests their derivation from an early uncommitted lymphoid cells.

Cytogenetic analysis of 434 consecutively ascertained specimens of non‐Hodgkin's lymphoma: Correlations between recurrent aberrations, histology, and exposure to cytotoxic treatment

“…In addition to t( 14; 18), t( 11; 14), and t(8; 14) and variants, two other specific translocations, t(3;22) and t(2;S) have been observed with significant frequency and have been linked to histologic subtypes of N H L Kaneko et al, 1989;Le Beau et al, 1989, Offit et al, 1989bEbrahim et al, 1990;Leroux et al, 1990). These studies have identified additional structural and numerical chromosome aberrations that often accompany the specific translocations.…”

“…Biopsy material was divided for histopathology, cytogenetic analysis, cell surface marker analysis, and gene rearrangement studies as previously described (Ebrahim et al, 1990). A subset of nine cases of mycosis fungoides were excluded from this analysis.…”

ATIC-ALK: A Novel Variant ALK Gene Fusion in Anaplastic Large Cell Lymphoma Resulting from the Recurrent Cryptic Chromosomal Inversion, inv(2)(p23q35)

“…4 The status of ALCL as a discrete entity had long been controversial, 5,6 and its recent separation into at least two subsets (see Discussion) stems from cytogenetic and molecular studies of the translocation seen in about 40 to 60% of cases, t(2;5)(p23;q35). [7][8][9] In 1994, the t(2;5) was found to involve a novel gene at 2p23 encoding a tyrosine kinase, ALK (anaplastic lymphoma kinase), and the NPM (nucleophosmin) gene at …”

Cytology of extranodal Ki‐1 anaplastic large cell lymphoma

“…38 Its molecular structure, namely the genes involved by the breakpoints, has recently been characterized, allowing detection of the translocation by the polymerase chain reaction. A recurrent translocation, t(2;5)(p23;q35), has recently been described in Ki-1 ALCL.…”