No abstract

We investigated 16 lymphoid proliferations occurring in the ocular adnexa, salivary glands, breast, and thyroid gland and satisfying the histopathologic and immunophenotypic criteria of benign lymphoid hyperplasia for the presence of clonal rearrangements of the antigen receptor, c-myc, bcl-1, and bcl-2 genes and Epstein-Barr virus (EBV) DNA sequences. Each of these 16 extranodal, noncutaneous lymphoid neoplasms exhibited clonal immunoglobulin heavy and/or light chain and lacked T-cell receptor (TCR) beta-chain gene rearrangements. The patterns of immunoglobulin gene rearrangements included solitary and multiple barely perceptible to faint bands, solitary clear and definite bands, and solitary high-intensity bands superimposed on a background of multiple less-intense bands. Three ocular adnexal lymphoid neoplasms exhibited bcl-1 or bcl-2 gene rearrangements. None of the 16 lymphoid neoplasms contained EBV DNA sequences. Two patients developed a histopathologically confirmed malignant lymphoma in an extranodal site. None of the remaining 14 patients developed additional lymphoid neoplasms during a mean follow-up period of 30 months, despite conservative therapy. These results demonstrate that extranodal, noncutaneous lymphoid neoplasms meeting the histopathologic and immunophenotypic criteria for benign lymphoid hyperplasia frequently contain occult monoclonal and oligoclonal B-cell populations representing a continuous and progressive spectrum of B-cell neoplasia up to and including malignant lymphoma.

Ki‐1‐positive anaplastic large‐cell lymphoma (ALCL) is an uncommon neoplasm which may present with extranodal as well as nodal disease. By definition, the tumor cells are immunoreactive for Ki‐1 or Ber‐H2 antigen (CD30). There have been few published cytologic descriptions of this lymphoma, or of its detection in extranodal sites. We describe the cytologic findings in five cases of extranodal Ki‐1 lymphoma. Cytologic findings in all five cases were similar and consisted of a heterogeneous population of lymphocytes and bizarre, pleomorphic tumor cells. These cells were characterized by generous amounts of vacuolated, basophilic cytoplasm, eccentric, multilobulated nuclei with some showing “wreath‐like” configurations. Some nuclei contained huge nucleoli simulating Reed‐Sternberg cells. All cases showed the characteristic surface membrane and cytoplasmic paranuclear dot‐like staining for CD30. Our findings indicate that fine‐needle aspiration and exfoliative cytology have a useful role in the diagnosis of Ki‐1 ALCL in extranodal sites. Furthermore, effusions containing anaplastic cells suspicious for lymphoma, particularly in AIDS patients, should be immunostained with antibodies to CD30. Diagn Cytopathol 1996;14:155–161. © 1996 Wiley‐Liss, Inc.

I-positive anaplastic large-cell lymphoma (ALCL) is an uncommon neoplasm which may present with extranodal as well as nodal disease. By definition, the tumor cells are immunoreactive for Ki-1 or Ber-H2 antigen (CD30). There have been few published cytologic descriptions of this lymphoma, or of its detection in extranodal sites. We describe the cytologic findings in five cases of extranodal Ki-1 lymphoma. Cytologic findings in all five cases were similar and consisted of a heterogeneous population of lymphocytes and bizarre, pleomorphic tumor cells. These cells were characterized by generous amounts of vacuolated, basophilic cytoplasm, eccentric, multilobulated nuclei with some showing "wreath-like" configurations. Some nuclei contained huge nucleoli simulating Reed-Sternberg cells. All cases showed the characteristic surface membrane and cytoplasmic paranuclear dotlike staining for CD30. Our findings indicate that fine-needle aspiration and exfoliative cytology have a useful role in the diagnosis of Ki-1 ALCL in extranodal sites. Furthermore, eflusions containing anaplastic cells suspicious for lymphoma, particularly in AIDS patients, should be immunostained with antibodies to CD30.